Biomedical subjects

Da-di Jin

Publications and source records attributed to Da-di Jin.

At least 19 recordsLinked to original sources

PubMed2006

[Analysis of the long-term outcome of anterior approach surgery on cervical spondylotic myelopathy].

OBJECTIVE: To investigate the long-term efficacy of anterior approach surgery on cervical spondylotic myelopathy and factors affecting prognosis. METHODS: The data in 116 patients suffered from cervical spondylosis from January 1992 to December 2000 were reviewed, including 80 male cases and 36 female cases, whose age ranged from 36 to 76 years (mean, 51 years). The preoperative course of disease was 2 months to 20 years (mean, 19 months). There were 65 cases (56.0%) with single segments involved, 44 cases (37.9%) with two segments, 7 cases (6.0%) with three segments. Ninety-eight cases were onset slowly, 18 cases with no remote cause and aggravating quickly. Three kinds of surgeries were performed: anterior cervical decompression and autoiliac bone interbody fusion, anterior cervical decompression and fusion with threaded fusion cage, anterior cervical decompression and autoiliac bone interbody fusion with anterior screw-plate system. Improvement in spinal cord function was assessed using the Japanese Orthopaedic Association (JOA) scoring system, the long-term efficacy and influential factors were also analyzed. RESULTS: The mean follow-up time was 7 years and three months (5 - 12 years). The mean preoperative JOA score was 9.34 +/- 1.81. The mean postoperative JOA score was 10.35 +/- 1.85. At the final follow-up, the JOA score was 14.09 +/- 1.90 and the recovery rate was 63.2%. Among the total patients, 27 cases were excellent, 47 cases were fine, 23 cases were good, 19 cases were poor, the fineness rate was 63.8%. The long-term efficacy of anterior approach surgery has close correlations with time of course, age of onset, preoperative spinal cord function and the number of affected segments, but has no correlations with modes of fusion and internal fixation. CONCLUSIONS: The patients will be attentively observed while having a definite diagnosis of cervical spondylotic myelopathy. The good long-term results will be obtained after early anterior cervical decompression and fusion.

Adult↗

PubMed2006

[Effects of Yunnan Baiyao on peri-operative bleeding of patients undergoing cervical open-door laminoplasty: a multi-center randomized double-blind placebo-control trial].

OBJECTIVE: To investigate the effects of Yunnan Baiyao on peri-operative bleeding of the patients undergoing cervical open-door laminoplasty. METHODS: 197 patients undergoing cervical open-door laminoplasty were randomly divided into 2 groups Yunnan Baiyao group (receiving Yunnan Baiyao 500 mg three times daily for 5 days) and placebo group. The amounts of intra-operative and post-operative bleeding were compared, and the side effect of Yunnan Baiyao was also analyzed. RESULTS: Statistical analysis showed that the amount of intra-operative bleeding of the Yunnan Baiyao was 350 ml +/- 190 ml, significantly lower than that of the placebo group (443 ml +/- 266 ml, P < 0.05). There were no statistical differences in the amount of postoperative bleeding and side effect rate between the two groups. CONCLUSION: Yunnan Baiyao is effective and safe in reducing the amount of intra-operative bleeding of cervical open-door laminoplasty.

Adult↗

PubMed2006

[Kyphoplasty using an enhanced balloon expander: an experimental study].

OBJECTIVE: To test the efficacy of kyphoplasty using an enhanced balloon expander in restoring the height of vertebral compression fractures (OVCF). METHODS: Fifteen lumbar vertebral bodies (L1-L5) were harvested from 3 young male fresh cadavers and separated into individual vertebral bodies with the bilateral pedicles of the vertebral arch removed. Before operation, plain X-ray films of all the vertebral bodies were obtained. All the vertebral bodies were compressed lengthwise to approximately 80% of their original heights using a universal material-testing machine to result in compression fractures. Post-compression vertebral bodies were then repaired using an enhanced balloon expander, and the delivery of the bone cement into the vertebral bodies was observed. The heights of the anterior and posterior borders of the vertebral bodied were measured before and after compression as well as after kyphoplasty. RESULTS: The inflation of the balloon expander averaged 2.95-/+0.18 ml and the pressure was 122.67-/+27.89 psi (1 psi=6895 Pa). Kyphoplasty resulted in significant restoration of the vertebral body height lost due to the compression (P<0.01). CONCLUSION: Kyphoplasty using an enhanced balloon expander may restore vertebral body height damaged by compression and correct the kyphotic deformity. The balloon expander can be a effective and economic choice for kyphoplasty for its relatively low cost.

Adult↗

PubMed2006

[Evaluation of perioperative blood loss following total knee arthroplasty].

OBJECTIVE: To evaluate perioperative occult blood loss following total knee arthroplasty (TKA). METHODS: A retrospective analysis of 40 patients undergoing TKA was conducted to calculate the mean blood loss and occult blood loss according to Gross formula. RESULTS: The mean total blood loss was 1538 ml in these cases with occult blood loss of 791 ml. In patients with autologous blood transfusion, the mean total blood loss was 1650 ml with occult blood loss of 786 ml. In patients without autologous blood transfusion, the mean total blood loss was 1370 ml with occult loss of 798 ml. CONCLUSION: TKA often results in large volume of occult blood loss in the perioperative period which can not be fully compensated by autologous blood transfusion, and additional blood supply is needed for maintenance of the circulating volume.

Aged↗

PubMed2005

[The effects of brain-derived neurotrophic factor gene-modified neural stem cells on primary cultured dorsal root ganglion cells in vitro].

OBJECTIVE: To gain stable genetic modification of neural stem cells (NSC) that constitutively secrete brain-derived neurotropic factor (BDNF) and to explore the biological role on the survival and neurite outgrowth of cultured dorsal root ganglion (DRG) neurons. METHODS: BDNF gene fragment from human brain cDNA libraries was obtained by using PCR. With molecular cloning technique, the recombinant stem cell viral vector with report gene was constructed, which is that MSCV-BDNF-IRES(2)-EGFP vector could encode Polycistronic mRNA. Viral particle was packaged by PT67 cell line and infected neural stem cells (mouse clone: C17.2). After selection with cloning cylinder, the expression of BDNF was assessed by immunohistochemistry enzyme linked immunosorbent assay (ELISA) and reverse transcriptase polymerase chain reaction (RT-PCR). The effects of stable gene-modified neural stem cells on embryonic mouse DRG neurons were evaluated in a dual-chambered cocultivation system at 3th, 10th day. RESULTS: RT-PCR analysis demonstrated expression of mRNA for human-BDNF. ELISA confirmed the presence of secreted BDNF 24 h after transfection and showed that the level of BDNF production by NSC-BDNF transfected C17.2 was at a rate of (14.6 +/- 0.8) ng x d(-1) x 10(-6) cells even after 3 months. With immunohistochemical analysis, compared with the control, the longer neurite outgrowth of cultured DRG cells and the more survival neurons were observed in NSC-BDNF transfected cells group. CONCLUSIONS: BDNF gene could be stably expressed in C17.2 cell line by MSCV, and the BDNF gene-modified NSC markedly enhances the survival and neurite outgrowth of cultured DRG neurons.

Animals↗

PubMed2005

[The role of cyclic AMP in repair of hemisection of spinal cord in rats models].

OBJECTIVE: To study the role of cAMP in repair of hemisection of spinal cord in rats models. METHODS: Rats models of spinal cord hemisection were made and cAMP were injected once in the motor cortex or continuously input in the lesion area or in the subarachnoid cistern for 3 d. NFs, GFAP, CSTs and spinal axons in the lesion areas were observed by immunohistochemistry and hind limb movements were evaluated in BBB scales. RESULTS: Many regenerated axons were presented in the lesion areas in cAMP groups though no continuous long regenerated axons traversed the lesion area when cAMP was input in the motor cortex or in the local lesion area. In control group, no regenerated axon were presented in the lesion areas. When cAMP was input in the subarachnoid cistern, only few-labelled CST axon survived and presented in the lesion area comparing no labelled CST axon presented in the lesion area. More NFs and less GFAP were distributed and extended in the lesion area in the cAMP groups. All the rats restored to normally walk 4-5 weeks after operations and no significance existed between cAMP groups and control groups comparing the BBB scales of hind limb movements. CONCLUSION: cAMP injected in the brain cortex or continuously input in the lesion area can induce the axonal regeneration.

Administration, Topical↗

PubMed2005

[Risk factor analysis of early complications following anterior spine surgery in 258 cases of cervical spondylotic myelopathy].

OBJECTIVE: To define the possible risk factors for early complications following anterior surgery for cervical spondylotic myelopathy. METHODS: Two hundred and fifty-eight cases of cervical spondylotic myelopathy receiving anterior surgery between January 1992 and December 2003 were reviewed and the gender, age and other factors of the patients were analyzed with single-factor analysis and multiple logistic regression. RESULTS: Complications arising in the early stage after the surgery were found in 19 cases (20 events), with an incidence rate of 7.4%;. Single-factor analysis showed that the two factors, paralysis and surgical approaches, were correlated with the early complications, and paralysis was identified as the major factor determining the occurrence of these complications by multiple logistic regression. CONCLUSION: Paralysis is the major factor related to the occurrence of early complications following anterior surgery for cervical spondylotic myelopathy, which reiterates the importance of proper perioperative management.

Adolescent↗

PubMed2004

[The selection of the surgical approach in the management of fracture and dislocation of lower cervical spine combined spinal cord injury].

OBJECTIVE: To investigate how to select an appropriate surgical approach in the management of fracture and dislocation of lower cervical spine combined spinal cord injury. METHODS: The clinical data of 54 patients of lower cervical spine fracture and dislocation were retrospectively analyzed. There were 29 cases with vertebral body compressive fracture and dislocation, 7 cases with vertebral body bursting fracture and dislocation, 3 cases with unilateral facet dislocation, 15 cases with bilateral facet dislocation. All cases were associated with spinal cord injury. According to American Spinal Injury Association (ASIA) grades, 21 cases were in A grade, 5 cases in B grade, 22 cases in C grade and 6 cases in D grade. All patients had surgical reduction, decompression, stabilization and fusion, 43 cases in anterior approach and 11 cases in posterior approach. RESULTS: All patients were followed up in 12 to 36 months, the mean follow-up time was 18 months. There were no great vessels, trachea, esophagus or spinal cord iatrogenic injury. There were no pull-out and breakage of screws or plates. Fusion was achieved in all patients at an average of 12 weeks postoperatively. There were no pseudarthrosis or bone nonunion. Of all the patients, 96.3% were acquired completely reduction and the normal intervertebral height and lordosis were maintained. Patients with complete spinal cord had no neurologic recovery, but they felt relief from upper limb pain or numb. Incomplete spinal cord lesions improved on average 1-2 Frankel grade after surgery. CONCLUSIONS: For lower cervical spine fracture and dislocation, an ideal anatomy reduction can be obtained with either anterior or posterior approach surgery. It is important to select a suitable surgical approach according to different types of cervical fracture and dislocation.

Adolescent↗

PubMed2004

[Molecular cloning and expression of human PDGF-B chain mature peptide gene].

OBJECTIVE: To acquire sufficient PDGF-BB protein and provide the basis for the further studies of its role on the fracture healing and trauma reconstruction and its clinical applications. METHODS: Constructed the prokaryotic expression vector pQE-PDGF-B with the gene rearrangement technique, and the monomeric form of recombinant PDGF-B expressed in E. coli M15. RESULTS: PDGF-B mature peptide gene was inserted into prokaryotic expression vector pQE30, which was confirmed by PCR, enzyme digestion and sequencing identification; the expressed products of pQE-PDGF-B in E. coli showed a single protein on SDS-PAGE, and their expression level was about 15% of the total bacterial protein. The molecular weight of the purified PDGF-B protein was about 15 KDs on SDS-PAGE. CONCLUSIONS: The construction of recombinant plasmid and preparation of the monomeric protein of PDGF-B provides a solid foundation for further studying the function of PDGF-BB and producing biologically PDGF-BB protein.

Becaplermin↗

PubMed2004

[Changes of nitric oxide content and nitric oxide synthase activity during rabbit cartilage endplate degeneration].

OBJECTIVE: To study the role of nitric oxide (NO) and nitric oxide synthase (NOS) in the degeneration of rabbit cartilage endplate (CEP). METHODS: Twenty-four New Zealand rabbits were divided into experiment (n=12) and control (n=12) groups, and in the former group rabbit models of lumbar CEP degeneration was established by resection of all the lumbar supraspinous and interspinous ligaments and excision of part of the zygapophyseal joints followed by detachment of the posterior paravertebral muscles from the lumbar vertebrae. Lumbar X-ray was performed in both groups 12, 24 and 36 weeks after operation, respectively. The content of NO and NOS activity in the CEP were also measured. RESULTS: X-ray of the CEP reveal gradual calcification of the cartilage endplate with the elapse of time, which was more obvious in the experiment group. The content of NO and NOS activity was significantly increased in the experiment group after the operation (P<0.05), but their difference between the measurements at 24 and 36 weeks postoperatively was not obvious (P>0.05). CONCLUSION: NO may play an important role in the development and progression of rabbit cartilage endplate degeneration.

Animals↗

PubMed2004

[Induced differentiation of human peripheral blood CD68+ mononuclear cells into osteoclasts in vitro].

OBJECTIVE: To establish a method for obtaining highly purified primary human osteoclast precursors for the biochemical and molecular biological research. METHODS: CD68(+) mono/macrophages were separated from peripheral blood mononuclear cells (PBMCs) of healthy donors by means of immunomagnetic cell sorting for subsequent analysis with flow cytometry. The isolated cells were incubated on coverslips or bone slices in the presence of dexamethasone(10(-8) mol/L), macrophage colony-stimulating factor (25 microg/L ) and soluble receptor activator of nuclear factor (NF)-kappaB ligand (s-RANKL, 16 microg/L). Calcitonin receptor (CR) immunocytochemistry and tartrate-resistant acid phosphatase (TRAP) histochemistry were employed. The bone slices were also studied by scanning electron microscopy (SEM). RESULTS: Fluorescence-activated cytometric analysis showed that 93.06%+/-0.61% n=4 of the selected cells were CD68(+) cells. After 7 days of culture of the CD68(+) cells, VR+, TRAP+ multinucleated giant cells appeared, and resorption lacunae could be observed by SEM. CONCLUSION: Highly purified CD68(+) cells can be obtained from human PBMCs as the osteoclast precursors, and mature osteoclasts can be induced from CD68(+) mono/macrophages by RANKL.

Adult↗

PubMed2004

[Diaphyseal defect repair with nacre/polylactic acid composite artificial bone in rabbits].

OBJECTIVE: To evaluate the biocompatibility, degradation and bone formation activity of a new bone substitute for bone grafting, nacre/polylactic acid composite artificial bone (NPCB). METHODS: Radial bone defects 1.5 cm in length were induced in 32 New Zealand rabbits and immediately filled with NPCB or nothing. The animals' local and whole body responses to the implants were observed after the operation, and the serum calcium levels were detected 1 week and 4 weeks postoperatively. Tissue response, new bone formation in the defects and degradation of the implants were evaluated by X-ray, and examination of the bone mineral content (BMC) in the defects and histomorphological analysis were performed after the rabbits were sacrificed. RESULTS: All the rabbits survived the operation and the incisions healed smoothly. No significant difference was noted in the serum calcium level between 1 day before operation, 1 week and 4 weeks postoperation. BMC in the defects of the rabbits with NPCB grafts increased more rapidly than that in the control group 6-12 weeks after the operation and began to decrease after 12 weeks. Gross, X-ray and histological observations revealed that NPCB possessed osteoconductive activity and new bone ingrowth in the implants was found during the whole experiment. At 16 weeks, the defects grafts were almost completely repaired with NPCB while the defects in the control group remained nonunion. Biodegradation of the implants was observed early at 6 weeks, with numerous macrophage and multinuclear giant cells containing phagocytosed NPCB particles around the implants at 12 weeks, but NPCB remnants were still visible at 16 weeks. CONCLUSION: As a bone substitute, NPCB possesses good biocompatibility, biodegradability and osteoconductive activity.

Absorbable Implants↗

PubMed2003

[Cloning and expression of insulin-like growth factor-1 platelet-derived growth factor-AA, and tumor growth factor beta1 genes in adenovirus vector].

OBJECTIVE: To establish recombinant adenovirus highly expressing genes of insulin-like growth factor-1 (IGF-1), platelet-derived growth factor-AA (PDGF-AA), and tumor growth factor beta1 (TGF beta 1) to be used in gene therapy. METHODS: Normal human osteoblasts were obtained from cancellous bone of lilium left from bone grafting and then cultured. Total RAN was extracted and IGF-1, PDGF-AA, and TGF beta 1 cDNAs was obtained by RT-PCR. Vector p-Shuttle containing these cNDAs was cloned into adenovirus and then the recombinant adenovirus was transfected into HEK293 cells. Recombinant adenovirus containing reporter gene LacZ was used in control group. Western blotting was used to detect the expression of these growth factors. Human osteoblasts were cultured and transfected with recombinant adenovirus. MTT method was used to detect the proliferation of the cells. Paranitrophenol method was used to examine the activity of alkaline phosphatase (ALP) in osteoblasts. RESULTS: Expression of IGF-1, PDGF-AA, and TGF beta 1 cDNA and expression of IGF-1, PDGF-AA, and TGF beta 1 proteins were found in HEK293 cells transfected with the recombinant adenovirus and not in the control HEK293 cells. The proliferation and ALP activity of osteoblasts transfected with the recombinant virus were significantly increased in comparison with those of the control osteoblasts (all P < 0.010). Immunohistochemical staining showed significant brown particles in the osteoblasts transfected with the recombinant virus and none in the control osteoblasts. CONCLUSION: The recombinant adenovirus thus constructed expresses the proteins of several growth factors with bioactivity in human cells and can be used as a satisfactory gene tool.

Adenoviridae↗

PubMed2003

[One-stage surgical management for spinal tuberculosis].

OBJECTIVE: To evaluate the clinical efficacy of one-stage surgical management for spinal tuberculosis. METHODS: Fifty-seven patients with thoracic, thoracolumbar and lumbar spinal tuberculosis were treated surgically by a single stage procedure from Jan 1996 to Jan 2001, including anterior resection, interbody outografting and anterior instrumentation in 35 cases, posterior debridement and posterior instrumentation in 16 cases, and posterior instrumentation and anterior resection plus interbody autografting in 6 cases. Titanium-alloy spinal implants were used in 38 cases and stainless steel implants in other 19 cases. Except for three patients, other 54 patients were followed up for one to five years (mean 2.2 years). RESULTS: All these patients had their incision healed by first intention without chronic infection or sinus formation, and no recurrence of spinal tuberculosis was noted in any patient during the follow-up period. Kyphosis correction were achieved by 21.6 degrees on the average postoperatively. There was a mild loss (2 degrees - 4 degrees) of kyphosis correction during follow-up period. Spinal solid fusion at the interface between the graft and adjacent vertebral bodies was shown radiologically in 3.8 months on the average. CONCLUSION: One-stage surgical management has more advantages in that it can completes all the aspects of surgical treatment for spinal tuberculosis. Anterior or posterior instrumentation with titanium-alloy or stainless steel spinal implants is safe, The selection of one-stage surgical procedure should be planed individually.

Adolescent↗

PubMed2003

[In vitro biocompatibility and degradation of nacre/polylactic acid composite artificial bone].

OBJECTIVE: To investigate the in vitro biocompatibility and degradation of nacre/polylactic acid composite artificial bone (NPCB) grafts. METHODS: Human osteoblast cells were cultured with NPCB discs for observation of the morphological features and cell proliferation by optical microscope and scanning electron microscope. The mass loss of NPCB discs and pH variations of the saline in which the discs were immersed were examined every 2 weeks in a course of 16 weeks in vitro. RESULT: Compared with the negative control group, NPCB showed no visible cytotoxicity and facilitated the growth and proliferation of the osteoblast cells. As the immersion prolonged, gradual decrease in the mass of NPCB was observed accompanied by regular pH alteration of the saline. CONCLUSION: NPCB possesses good in vitro biocompatibility and may be gradually degraded.

Biocompatible Materials↗

PubMed2003

Anterior decompression and instrumentation with cervical butterfly-like locking plate system for herniated cervical intervertebral discs.

OBJECTIVE: To evaluate the clinical efficacy of anterior decompression and internal fixation with cervical butterfly-like locking plate system (CBLP, made in China) in the treatment of cervical intervertebral disc herniation. METHODS: This study included 16 patients with cervical intervertebral disc herniation, aged from 26 to 46 years (36.2 years on average), with the disc hernia occurring in single segment in 12 and 2 segments in 4 cases. The patients received anterior decompression, autogenous iliac bone grafting and internal fixation with CBLP, and follow-up studies lasting for 12 to 24 months (mean 18 months) were conducted in all the cases. RESULTS: All the patients had significant improvements in terms of symptom alleviation without any complications during the follow-up period. Solid bone fusion was achieved within an average period of 3 months, and no screw loosening or plate failure occurred. CONCLUSION: Anterior decompression and internal fixation with CBLP proves to be effective for managing cervical intervertebral disc herniation.

Adult↗

PubMed2003

[Growth factor and regeneration of intervertebral disc].

OBJECTIVE: To review research progress of the relation between growth factor and repair of intervertebral disc. METHODS: The recent articles on growth factor and repair of intervertebral disc were extensively reviewed. The expression of growth factor in intervertebral disc and the effect of growth factor on disc cells were investigated. RESULTS: Some growth factors play roles in the development and degeneration of intervertebral disc. Exogenous growth factor can increase proliferation of disc cells and production of proteoglycans and collagens. Gene of growth factor can be transferred to intervertebral disc cell by adenovirus. CONCLUSION: Growth factor plays an important role in the regulation of development and degeneration of interertebral disc. The above results show that the feasibility of usage of growth factor in the treatment of disc degeneration and in repair and reconstruction of disc.

Cell Division↗

PubMed2003

[Treatment of spondylolysis and spondylolisthesis with posterior STB thoracolumbar transpedicular screw-plate spine fixation system].

OBJECTIVE: To observe the clinical effect of a self-designed posterior STB thoracolumbar transpedicular screw-plate system in the treatment of spondylolysis and spondylolisthesis. METHODS: This STB screw-plate system is developed with titanium alloy (TC4, Ti6Al4V). During December, 1999 and January, 2001, this system was applied in 51 cases, including lumbar spondylolysis and spondylolisthesis (36 cases), degenerative lumbar instability (15 cases). The patients were aged 22 - 78 years, mean age: 47.5 - years; among them there were 14 male cases, 37 female cases; There were 15 cases degenerative spondylolisthesis with grade I-II slip, 36 cases of spondylolysis and spondylolisthesis, including 34 cases with grade I-II slips and 2 cases with grade III slips; single level of 33 cases, two levels 3 cases. Decompression was performed for the 43 cases with interbody fusion using iliac crest or bilateral-lateral fusion using demineralized bone matrix and 8 cases were fixed with demineralized bone matrix fusion without decompression. RESULTS: All the operations heeded about 60 - 120 minutes, and during the operations bleeding amounts were 200 - 500 ml. The cases completely recovered to work or normal action after 3 months of surgeries. 46 of all cases were achieved to satisfactorily reduction and clinical effect, 5 cases of grade II-III remained incomplete reduction (grade I slip). CONCLUSION: This STB system has advantages of biomechanical stability and reduction capability and its effect in clinical application is undoubtedly positive.

Adult↗