Biomedical subjects

Dale Kaiser

Publications and source records attributed to Dale Kaiser.

24 records · Page 2Linked to original sources

PubMed2004

Dynamics of fruiting body morphogenesis.

Myxobacteria build their species-specific fruiting bodies by cell movement and then differentiate spores in specific places within that multicellular structure. New steps in the developmental aggregation of Myxococcus xanthus were discovered through a frame-by-frame analysis of a motion picture. The formation and fate of 18 aggregates were captured in the time-lapse movie. Still photographs of 600 other aggregates were also analyzed. M. xanthus has two engines that propel the gliding of its rod-shaped cells: slime-secreting jets at the rear and retractile pili at the front. The earliest aggregates are stationary masses of cells that look like three-dimensional traffic jams. We propose a model in which both engines stall as the cells' forward progress is blocked by other cells in the traffic jam. We also propose that these blockades are eventually circumvented by the cell's capacity to turn, which is facilitated by the push of slime secretion at the rear of each cell and by the flexibility of the myxobacterial cell wall. Turning by many cells would transform a traffic jam into an elliptical mound, in which the cells are streaming in closed orbits. Pairs of adjacent mounds are observed to coalesce into single larger mounds, probably reflecting the fusion of orbits in the adjacent mounds. Although fruiting bodies are relatively large structures that contain 10(5) cells, no long-range interactions between cells were evident. For aggregation, M. xanthus appears to use local interactions between its cells.

Morphogenesis↗

PubMed2004

Signaling in myxobacteria.

Myxobacteria use soluble and cell-contact signals during their starvation-induced formation of fruiting bodies. These signals coordinate developmental gene expression with the cell movements that build fruiting bodies. Early in development, the quorum-sensing A-signal in Myxococcus xanthus helps to assess starvation and induce the first stage of aggregation. Later, the morphogenetic C-signal helps to pattern cell movement and shape the fruiting body. C-signal is a 17-kDa cell surface protein that signals by contact between the ends of two cells. The number of C-signal molecules per cell rises 100-fold from the beginning of fruiting body development to the end, when spores are formed. Traveling waves, streams, and sporulation have increasing thresholds for C-signal activity, and this progression ensures that spores form inside fruiting bodies.

Myxococcus xanthus↗

PubMed2004

Pulling together with type IV pili.

Type IV pili are an efficient and versatile device for bacterial surface motility. They are widespread among the beta-, gamma-, and delta-proteobacteria and the cyanobacteria. Within that diversity, there is a core of conserved proteins that includes the pilin (PilA), the motors PilB and PilT, and various components of pilus biogenesis and assembly, PilC, PilD, PilM, PilN, PilO, PilP, and PilQ. Progress has been made in understanding the motor and the secretory functions. PilT is a motor protein that catalyzes pilus retraction; PilB may play a similar role in pilus extension. Type IV pili are multifunctional complexes that can act as bacterial virulence factors because pilus-based motility is used to spread pathogens over the surface of a tissue, or to build multicellular structures such as biofilms and fruiting bodies.

Bacteria↗

PubMed2003

Coupling cell movement to multicellular development in myxobacteria.

The myxobacteria are Gram-negative organisms that are capable of multicellular, social behaviour. In the presence of nutrients, swarms of myxobacteria feed cooperatively by sharing extracellular digestive enzymes, and can prey on other bacteria. When the food supply runs low, they initiate a complex developmental programme that culminates in the production of a fruiting body. Myxobacteria move by gliding and have two, polarly positioned engines to control their motility. The two engines undergo coordinated reversals, and changes in the reversal frequency and speed are responsible for the different patterns of movement that are seen during development. The myxobacteria communicate with each other and coordinate their movements through a cell-contact-dependent signal. Here, the cell movements that culminate in the development of the multicellular fruiting body are reviewed.

Morphogenesis↗

PubMed2002

How myxobacteria glide.

BACKGROUND: Many microorganisms, including myxobacteria, cyanobacteria, and flexibacteria, move by gliding. Although gliding always describes a slow surface-associated translocation in the direction of the cell's long axis, it can result from two very different propulsion mechanisms: social (S) motility and adventurous (A) motility. The force for S motility is generated by retraction of type 4 pili. A motility may be associated with the extrusion of slime, but evidence has been lacking, and how force might be generated has remained an enigma. Recently, nozzle-like structures were discovered in cyanobacteria from which slime emanated at the same rate at which the bacteria moved. This strongly implicates slime extrusion as a propulsion mechanism for gliding. RESULTS: Here we show that similar but smaller nozzle-like structures are found in Myxococcus xanthus and that they are clustered at both cell poles, where one might expect propulsive organelles. Furthermore, light and electron microscopical observations show that slime is secreted in ribbons from the ends of cells. To test whether the slime propulsion hypothesis is physically reasonable, we construct a mathematical model of the slime nozzle to see if it can generate a force sufficient to propel M. xanthus at the observed velocities. The model assumes that the hydration of slime, a cationic polyelectrolyte, is the force-generating mechanism. CONCLUSIONS: The discovery of nozzle-like organelles in various gliding bacteria suggests their role in prokaryotic gliding. Our calculations and our observations of slime trails demonstrate that slime extrusion from such nozzles can account for most of the observed properties of A motile gliding.

Cyanobacteria↗

PubMed2002

act operon control of developmental gene expression in Myxococcus xanthus.

Cell-bound C-signal guides the building of a fruiting body and triggers the differentiation of myxospores. Earlier work has shown that transcription of the csgA gene, which encodes the C-signal, is directed by four genes of the act operon. To see how expression of the genes encoding components of the aggregation and sporulation processes depends on C-signaling, mutants with loss-of-function mutations in each of the act genes were investigated. These mutations were found to have no effect on genes that are normally expressed up to 3 h into development and are C-signal independent. Neither the time of first expression nor the rate of expression increase was changed in actA, actB, actC, or actD mutant strains. Also, there was no effect on A-signal production, which normally starts before 3 h. By contrast, the null act mutants have striking defects in C-signal production. These mutations changed the expression of four gene reporters that are related to aggregation and sporulation and are expressed at 6 h or later in development. The actA and actB null mutations substantially decreased the expression of all these reporters. The other act null mutations caused either premature expression to wild-type levels (actC) or delayed expression (actD), which ultimately rose to wild-type levels. The pattern of effects on these reporters shows how the C-signal differentially regulates the steps that together build a fruiting body and differentiate spores within it.

Bacterial Proteins↗