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Biomedical subjects

Daniel A Davis

Publications and source records attributed to Daniel A Davis.

6 recordsLinked to original sources
PubMed2006

Combination forceps fuse both safety and efficiency.

BACKGROUND: Instrumentation prevents needle stick injury. OBJECTIVE: To review forceps that insure safety and facilitate tissue-handling and knot-tying efficiency. METHOD: Medical literature reports were reviewed using Ovid. Commercially available instruments were qualitatively tested. RESULTS: Suture platforms securely hold suture needles and can be used during knot tying. A wide range of combination forceps have been invented and can be broadly categorized as either skin hook or toothed combination forceps. CONCLUSIONS: Combination forceps fuse both efficiency and safety. Skin hook forceps may eventually be the optimal combination instrument, but toothed combination forceps are recommended.

Equipment Design↗
PubMed2005

Ultrarapid staining for cutaneous melanoma: study and protocol.

BACKGROUND: Immunohistochemistry has revolutionized the use of frozen sections for the removal of melanoma. OBJECTIVE: Two studies were performed to address the best and fastest way to stain melanocytes in frozen tissue sections. MATERIALS AND METHODS: Fifty-six standing cones were taken from sun-damaged white skin and stained with combinations of anti-S-100, anti-HMB-45, anti-Melan-A, and azure B with appropriate controls. RESULTS: Anti-Melan-A was the best immunohistochemical stain. Azure B stains melanin granules metachromatically green and allows differentiation of melanocytes and melanophages. Ultrarapid staining worked as well as rapid staining. CONCLUSION: Dako's EnVision ultrarapid staining protocol and azure B counterstain represent a dramatic improvement and should be considered the easily obtainable histologic standard when excising cutaneous melanoma.

Frozen Sections↗
PubMed2005

The diagnostic concordance of actinic keratosis and squamous cell carcinoma.

Diagnostic concordance of intraepithelial malignancy is generally only fair. Because the diagnosis of actinic keratosis (AK) and squamous cell carcinoma (SCC) is not uniform and because such terms are not consonant with the nomenclature of other human epithelial malignancies, nomenclature revisions have been attempted. One hundred dermatopathologists were solicited to review 15 tissue sections representing a spectrum of varying thickness epidermal malignancy and to choose either AK or SCC as the diagnosis. Among the 77 participating dermatopathologists, intraclass correlation was high for what was perceived as AK, SCC, and their differentiation. Development of a two-tiered diagnostic system that retains our present diagnostic capabilities, but better fits the pathobiology of superficial epidermal malignancy is suggested. Davis DA, Donahue JP, Bost JE, Horn TD. The diagnostic concordance of actinic keratosis and squamous cell carcinoma.

Carcinoma in Situ↗
PubMed2004

Preparation of frozen sections.

BACKGROUND: For dermatologists the preparation of frozen sections means micrographic surgery. The preparation of frozen sections is meant to be rapid, but has multiple, discrete steps that demand attention to detail. OBJECTIVE: The objective was to summarize both the proven and the cutting-edge techniques of frozen section preparation. MATERIALS AND METHODS: Reports from the medical literature were used to augment techniques used in our practices. CONCLUSION: We dissect each step of micrographic cancer removal, report on the standard and preferred methods of tissue preparation, and summarize the progress achieved in the past decade.

Frozen Sections↗
PubMed2002

1, 2, 3, 4: four techniques to secure buried knots.

BACKGROUND: High tension wounds can be difficult to close. OBJECTIVE: To enumerate methods for securing a surgeon's knot when tying buried sutures under tension. CONCLUSION: The tension tranfer stitch is a swift and elegant method to secure surgeon's knots.

Humans↗