Biomedical subjects

Dariusz Dobrowolski

Publications and source records attributed to Dariusz Dobrowolski.

5 recordsLinked to original sources

PubMed2006

[Results of posterior lamellar keratoplasty].

PURPOSE: Posterior lamellar keratoplasty (PLK) in patients with pseudophakic corneal oedema. PARTICIPANTS AND METHODS: The PLK have been performed in our department since November 2004. The studied group consisted of 8 patients (7 female and 1 male), 65 to 82 years of age (mean age: 71.5 years). Visual acuity before the surgery ranged from 0.01 to fingers counting. All the patients suffered from pseudophakic corneal oedema. Endothelial keratoplasty was performed using Melles technique: a deep stromal pocket was created across the cornea through a 5-mm scleral access incision. Endothelium was prepared from whole eyeballs collected according to the EEBA guidelines. Donor disc containing healthy endothelium was folded during the implantation procedure. Average donor endothelial cell density was 2800 cells/mm2. All surgeries were performed by one surgeon (EW). RESULTS: In two eyes conversion to PKP was necessary. Best corrected visual acuity (BCVA) ranged from 0.4 to 1.0 (mean 0.6 +/- 0.12). Postoperative astigmatism ranged from 0.75 to 2.5 D (mean 1.8 D +/- 0.34). Postoperative endothelial cell density ranged from 1700 do 2300 cells/mm2 (mean 2200 cells/mm2 +/- 150). Mean Postoperative endothelial cell loss came to 29%. CONCLUSIONS: Posterior lamellar keratoplasty is a feasible surgery to manage corneal endothelial disorders. Automation of procedures by the use of laser preparation of donor tissue and adaptation automatic microkeratomes will increase the afterrepeating of procedures.

Aged↗

PubMed2005

[Severe exacerbation of chronic renal failure in the course of asymptomatic cystolithiasis].

There has been reported a case of severe exacerbation of chronic renal failure with bilateral hydronephrosis and urosepsis, which was caused by asymptomatic large urinary bladder stone. Life-threatening symptoms of uraemic syndrome were found, which required temporary hemodialysis treatment. Following removal of the calculus and controlling of severe urinary tract infection the patient was discharged with stable blood serum creatinine concentration 3.8 mg/dl.

Creatinine↗

PubMed2005

[Indications for keratoplasty in District Railway Hospital in Katowice].

PURPOSE: The retrospective analysis of indications for keratoplasty. MATERIAL AND METHODS: The study of 517 keratoplasties between May 2000 and December 2004. RESULTS: The indications were: corneal oedema--24,5%, keratoconus--18,1%, corneal leucoma--15,0%, limbus insufficiency--10,2%, Fuchs endothelial dystrophy--9,0%, graft decompensation--8,1%, other acquired keratopaties--5,9%, corneal perforation--5,8%, anterior and stromal dystrophies--2,3%, Peters' syndrome--0,38%, choristoma--0,19%. CONCLUSION: Main indication for keratoplasty are corneal oedema and keratoconus.

Corneal Diseases↗

PubMed2004

[Macular corneal dystrophy including histologic and ultrastructural changes].

PURPOSE: The study reports the results of a histological and ultrastructural examination of the corneal button, obtained during penetrating keratoplasty from patient with clinically recognized macular corneal dystrophy. MATERIAL AND METHODS: 34-year-old male patient suffering from macular corneal dystrophy (MCD) has been treated on corneal epithelium defect and photophobia since his early childhood. Visual acuity was decreased on the Snellen test chart to 0.02. Slit-lamp examination, and ultrasonographical measurement of the cornea's thickness were performed. Removed during penetrating keratoplasty corneal button was divided into two pieces. One of them was prepared in standard procedure for histological examination in the light microscopy after having been stained with hematoxylin and eosin, alcian blue and paS-method. From the other part, slides for ultrastructural examination in the transmission electron microscopy were prepared with the use of standard method. The family history from the patient was also taken, and available relatives have undergone examination in search of typical MCD symptoms. RESULTS: Slit-lamp examination findings revealed diffuse, from limbus to limbus, stromal opacification. In measurement by pachymeter cornea's thickness was reduced. In the light microscopy, in typical stained slides, delaminations within stroma and deficit of endothelial cells were observed. After being stained with alcian blue, dark blue deposits in the places of delamination became visible. By transmission electron microscopic examination, intracellular and extracellular deposits were detected in the stroma, Descemet membrane and endothelium. Distended keratocytes with enormous vacuoles containing abnormal material were found. Pedigree was typical for autosomal recessive inherited disease. CONCLUSIONS: Histological and ultrastructural diagnosis is a basis of recognition of macular corneal dystrophy. Analysis of the pedigree as well as ultrasonographical measurement of the cornea's thickness is very helpful to establish the right diagnosis.

Adult↗

PubMed2004

[Culture of the corneal epithelium--comparison of the mitotic potential of limbal cells from living and cadaveric donors].

PURPOSE: We analyse effectiveness of corneal limbal cells' culture prepared from heart-beating organ donors, that include living donors and from cadaver donors buttons following 3 days storage in 4 degrees C in Likorol. MATERIAL AND METHODS: For experiment 12 adults (living and heart-beating organ donors) aged 28-63 (mean 46.3) and 12 corneal buttons of cadaver (aged 18-51, mean 34.1) were qualified. Tissue samples (1 mm2) were taken from superior corneal limbus. Sample from living donor obtained during routine operation was sent immediately to laboratory, as well as from heart-beating organ donor. The limbal biopsy of preserved cornea was taken after 3 days storage in 4 degrees C (Likorol). Samples were treated with trypsin/EDTA solution before culture. Collected cells in similar density in 1 ml of medium laid on dishes inserts, covered with fibrin (Tissucol) and cultured in presence of feeder layer of fibroblasts (L929 line). Epithelial cells were cultured for 14 days at 37 degrees C in humidified 5% CO2 atmosphere in supplemented 2:1 mixture of DMEM and Ham's F12. On the 14th day cells were collected. Number of cells per 1 ml of medium was counted by cytometer. Immunostaining for epithelium type (Keratin 3) was performed. RESULTS: The number of cells obtained from cadaver donors reached 184.2+/-14.9% whereas from living donors revealed 1013.1+/-104.2%, increase in relation to number of delivered cells. We observed only 0.83+/-0.3 colonies per microscopic area in cultures from preserved tissue versus 6.67+/-0.6 colonies in cultures from living donor. CONCLUSIONS: The preservation in 4 degrees C in Likorol significantly decreases proliferative potential of the corneal limbus.

Adult↗