Biomedical subjects

David A Kidwell

Publications and source records attributed to David A Kidwell.

3 recordsLinked to original sources

PubMed2004

An enzyme electrode for amperometric measurement of D-amino acid.

A carbon paste enzyme electrode has been developed for measurement of D-amino acids that employs a fatty acid modified FAD to prevent leaching of this essential cofactor to the surrounding aqueous environment and which serves as an enzyme stabilizing agent. The lower limit of detection is at least 10(-4) M and the electrode has a linear range of 10(-4) to 3 x 10(-3) M and a response time of 180 s. Twenty L-amino acids were tested and none of them elicited responses when electrodes were exposed to 0.5 mM concentration increases over a baseline level. On the other hand, some response was observed when exposed to 18 of 20 D-amino acids varying from 2 to 200% of the corresponding D-alanine response. Electrodes were shown to have longevities of over 30 days while maintaining 85% of their original sensitivity. Electrodes showed activity over a pH of 6.2-11.7 with a maximum at 9.2 and over temperatures of 10-47 degrees C with a maximum at 37 degrees C.

Amino Acids↗

PubMed2004

Comparing two analytical methods: minimal standards in forensic toxicology derived from information theory.

Frequently, new instrumentation and techniques are being applied to casework without an adequate knowledge of how this new technology compares to acceptable analytical procedures. Information theory provides a mathematical method to estimate the identification power of various analytical procedures and predicts if the new method produces a better or poorer confidence in the analysis than an accepted method. This paper makes comparisons based on relative informational power and discusses methods to make those estimates. With the procedures outlined in this paper, an analyst can estimate if a novel technique has the opportunity of reaching the accepted analytical methodology and, if necessary, identify places where the greatest improvement can be made to reach this equilivalence. Even though relative information theory can dismiss some proposed techniques before any analysis starts, it cannot verify if a given technique is adequate for a particular task. For that purpose, a complete procedural verification must be undertaken.

Forensic Medicine↗

PubMed2004

Model-based optimization of a conductive matrix enzyme electrode.

A mathematical model has been developed to describe the mechanism for internal mass transfer and enzyme reaction kinetics of an amperometric conductive matrix enzyme electrode. The model is simplified and solved analytically to arrive at a representation for the response slope in the linear range as well as for the response time. This is the first time that the response time of an enzyme electrode is described by a mathematical model. Simulations give information on how the design parameters influence the performance of the electrode for a glucose oxidase catalyzed sensing reaction process. Based on this information, several designs were constructed and tested showing suitable agreement with theoretical predictions. Finally, an optimized electrode was designed and validated.

Biosensing Techniques↗