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Biomedical subjects

David E Wolf

Publications and source records attributed to David E Wolf.

4 recordsLinked to original sources
PubMed2003

Quantitative digital and video microscopy.

Obviously there are many variations and embellishments on the topic of quantitating your image. I have tried here to offer you a very practical guide that highlights some of the critical issues, paying particular attention to problems that can prevent or compromise your success. These include errors caused by aspect ratio, use of automatic camera settings, improper setting of dynamic range, and use of integer arithmetic. Further information can be found in several other chapters in this volume as well as in the references provided. In addition, you should not overlook the technical manuals that come with your imaging systems. The field of digital microscopy has evolved as a cooperative effort between academia and industry. As a result you will find that many of the technical support personnel from microscope, camera, and image-processing companies are well versed on the issue of digital imaging in biology and are more than willing to assist you.

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PubMed2003

Fluorescence correlation spectroscopy: molecular complexing in solution and in living cells.

FCS is an important technique for biophysicists, biochemists, and cell biologists. FCS represents an example of how one can make use of the microscope and electronics to extract information beyond the resolution limit of classical optics. It can be used to study single-molecules both in solution and in living cells and can be used to monitor a wide variety of macromolecular interactions. When used as an in vitro technique, FCS measurements are easy to conduct and can be made on simplified instrumentation. When used in vivo on living cells, many additional factors must be considered when evaluating experimental data. Despite these concerns, FCS represents a new approach that has broad applicability for the determination of molecular stoichiometry both in vivo and in vitro for a variety of membrane and soluble receptor systems.

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