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David Schmidt

Publications and source records attributed to David Schmidt.

6 recordsLinked to original sources
PubMed2026

CERTOMICS: trusted single-cell multiomics pipeline for high-resolution profiling of adoptive cellular immunotherapies.

SUMMARY: Adoptive cellular immunontherapies, such as chimeric antigen receptor (CAR) T cell therapy, have transformed cancer treatment, yet challenges such as resistance, relapse, and high costs limit their efficacy and accessibility. A comprehensive understanding of cellular heterogeneity and molecular profiles is essential to improve these therapies. Advanced single-cell multiomics technologies have the power to analyze the complex interactions between CAR-engineered cells, immune cells, and tumor cells. However, standardized single-cell multiomics computational pipelines specifically tailored to CAR-engineered cell products are lacking. Due to the synthetic nature of CAR transgenes, additional steps for reliable identification and characterization of CAR-positive cells are required but not included in existing data-processing workflows. To address this, we present CERTOMICS, a Nextflow-based, CAR-aware pipeline offering enhanced CERTainty in immunophenotyping and data interpretation, tailored for single-cell multiOMICSprofiling of adoptive cellular immunotherapies. The pipeline standardizes processing 10x Genomics single-cell multiomics data and integrates CAR-specific identification and quality control. Additionally, a curated repository of CAR construct sequences and annotation data is provided, serving as an extensible resource to support the analysis and development of CAR T cell therapies. AVAILABILITY AND IMPLEMENTATION: Detailed documentation of this pipeline, along with a resource on latest FDA-approved CAR therapies is available on our website: https://fraunhofer-izi.github.io/Living-Drugs-Wiki/. The data underlying this article are available on GitHub at https://github.com/fraunhofer-izi/CERTOMICS. The code is also published on Zenodo at https://doi.org/10.5281/zenodo.18709693.

Multiomics↗
PubMed2005

Obstetrical complications in gestational carrier pregnancies.

OBJECTIVE: To report two cases of severe obstetrical complications in gestational carrier pregnancies and to review our clinical experience and compare our results with those reported in the literature. DESIGN: Retrospective analysis. SETTING: A university IVF program. PATIENT(S): Women without a functioning uterus or those whose pregnancy would exacerbate a medical condition were enrolled in the gestational carrier pregnancy program. INTERVENTION(S): IVF cycles using oocytes from genetic mothers (or oocyte donors) were performed, with ET to gestational carriers. MAIN OUTCOME MEASURE(S): Clinical pregnancy rates, obstetrical complications, and neonatal outcomes. RESULT(S): Ten couples underwent a total of 13 cycles using gestational carriers. A clinical pregnancy rate of 69% (9/13) was achieved. An intrapartum hysterectomy and a late puerperal hysterectomy were required because of severe obstetrical complications. The late puerperal hysterectomy was performed for placenta accreta in a triplet gestation. This carrier sustained multiple cerebral infarcts and blindness. One triplet infant died secondary to a hypoplastic left ventricle and complications of prematurity. A second gestational carrier with a singleton gestation underwent a hysterectomy for a uterine rupture, and the infant has cerebral palsy. CONCLUSION(S): The past medical and obstetrical histories of potential gestational carriers must be closely scrutinized, and candidates must be thoroughly counseled about the potential risks involved in the procedure.

Female↗
PubMed2005

Outcome of in vitro fertilization treatment in patients who electively inseminate a limited number of oocytes to avoid creating surplus human embryos for cryopreservation.

OBJECTIVE: To determine whether the outcome of IVF treatment in patients who electively inseminate a limited number of oocytes is comparable to that in a group of good-prognostic or poor-prognostic patients. DESIGN: Retrospective clinical study. SETTING: University-based tertiary fertility center. PATIENT(S): Two hundred three women under the age of 40 years. INTERVENTION(S): Patients undergoing their first cycle of IVF who elected to have no more than four oocytes inseminated (study group) or who produced only four or fewer oocytes (poor-prognostic group) or who had excess embryos cryopreserved (good-prognostic group). MAIN OUTCOME MEASURE(S): Implantation rate, clinical pregnancy rate, and ongoing pregnancy rate. RESULT(S): There were no significant differences in the implantation rate for the study group when compared with the good-prognostic group or the poor-prognostic group. The clinical pregnancy rate (62.5% vs. 64%) and ongoing-pregnancy or birth rate (56.3% vs. 60.7%) were similar between the study group and the good-prognostic group. However, the clinical pregnancy rate (62.5% vs. 29.7%) and ongoing-pregnancy rate (56.3% vs. 24.3%) were higher in the study group compared with the poor-prognostic group. CONCLUSION(S): Inseminating fewer oocytes in patients who elect not to cryopreserve excess embryos does not adversely affect their probability of conception.

Adult↗
PubMed2004

An unusual anatomic variation of a unicornuate uterus with normal external uterine morphology.

OBJECTIVE: To describe a case of a unicornuate uterus with a normal external uterine morphology. DESIGN: Case report. SETTING: University-based fertility center. PATIENT(S): A 30-year-old nulligravid woman with a 1-year history of infertility found to have a right proximal tubal occlusion on hysterosalpingogram. INTERVENTION(S): Laparoscopy, hysteroscopy, and magnetic resonance imaging. MAIN OUTCOME MEASURE(S): External and internal morphology of the uterus. RESULT(S): Laparoscopy showed a normal external uterine morphology and normal fallopian tubes and ovaries, but chromopertubation failed to demonstrate a fill and spill from the right fallopian tube. Hysteroscopy showed a single tubular uterine cavity projecting to the left with a single left tubal ostium, consistent with a unicornuate uterus. Magnetic resonance imaging confirmed a normal external uterine fundal contour and an internal uterine morphology consistent with a unicornuate uterus. CONCLUSION(S): This is the first reported case of a unicornuate uterus presenting with a normal external uterine morphology and an internal morphology consistent with a unicornuate uterus, and we propose inclusion of this anomaly in the classification of mullerian anomalies.

Adult↗