Biomedical subjects

Diana Popa

Publications and source records attributed to Diana Popa.

9 recordsLinked to original sources

PubMed2006

Molecular basis of age-associated cytokine dysregulation in LPS-stimulated macrophages.

Aged humans and rodents are susceptible to infection with Streptococcus pneumoniae bacteria as a result of an inability to make antibodies to capsular polysaccharides. This is partly a result of decreased production of proinflammatory cytokines and increased production of interleukin (IL)-10 by macrophages (Mphi) from aged mice. To understand the molecular basis of cytokine dysregulation in aged mouse Mphi, a microarray analysis was performed on RNA from resting and lipopolysaccharide (LPS)-stimulated Mphi from aged and control mice using the Affymetrix Mouse Genome 430 2.0 gene chip. Two-way ANOVA analysis demonstrated that at an overall P < 0.01 level, 853 genes were regulated by LPS (169 in only the young, 184 in only the aged, and 500 in both). Expression analysis of systematic explorer revealed that immune response (proinflammatory chemokines, cytokines, and their receptors) and signal transduction genes were specifically reduced in aged mouse Mphi. Accordingly, expression of Il1 and Il6 was reduced, and Il10 was increased, confirming our previous results. There was also decreased expression of interferon-gamma. Genes in the Toll-like receptor-signaling pathway leading to nuclear factor-kappaB activation were also down-regulated but IL-1 receptor-associated kinase 3, a negative regulator of this pathway, was increased in aged mice. An increase in expression of the gene for p38 mitogen-activated protein kinase (MAPK) was observed with a corresponding increase in protein expression and enzyme activity confirmed by Western blotting. Low doses of a p38 MAPK inhibitor (SB203580) enhanced proinflammatory cytokine production by Mphi and reduced IL-10 levels, indicating that increased p38 MAPK activity has a role in cytokine dysregulation in the aged mouse Mphi.

Aging↗

PubMed2006

[Mechanisms of laser photocoagulation in diabetic retinopathy].

The aim of laser photocoagulation in diabetic retinopathy is to stop the development of retinal neovessels and even to determine their involution. Early applied, laser photocoagulation can significantly reduce the risk of severe visual loss. The knowledge of the multiple mechanisms of laser photocoagulation leads to the possibility to introduce a well dosed treatment that is appropriate for the clinical stage of the retinopathy.

Diabetic Retinopathy↗

PubMed2006

[Our experience with Acrysof Natural intraocular lens].

UNLABELLED: The purpose of this paper is to appreciate the Acrysof Natural IOL comportment during the implantation after phacoemulsification, and to evaluate the visual acuity at 2 months and 2 years postoperative. MATERIAL AND METHODS: Prospective clinical study which comprises 48 patients( 48 eyes) with cataract operated on with phacoemulsification and implantation of Acrysof Natural IOL with the Monarch II injector and the implantation forceps. RESULTS: The patients' age was between 4- 82 years; The IOLs were introduced with the Monarch II injector in 47 cases and with the implantation forceps in 1 case; No IOL was damaged during the implantation; The unfolding and the rotation of the IOL were possible in all cases, without incidents; The visual acuity appreciated 2 months and 2 years postoperative was good in the most of the cases; the chromophore pigment did not influence the quality of vision and the color perception. CONCLUSIONS: The implantation of the Acrysof Natural IOL is identical with the implantation of the Acrysof single piece IOL, is easy to perform and completely atraumatic.

Acrylic Resins↗

PubMed2005

[Phacoemulsification with Advan-Tec system and Neosonix handpiece].

PURPOSE: The comparative analyses of the standard Legacy 20000 efficiency and the AdvanTec Legacy efficiency take into consideration the effective phacoemulsification time, power, and the postoperative clinical outcomes. MATERIAL AND METHODS: Retrospective clinical study which comprises 2 groups of patients operated on with phacoemulsification: 200 eyes were operated on using the classic handpiece of the Legacy 20000 system; 200 eyes were operated on using the AdvanTec Legacy system and the NeoSoniX handpiece. RESULTS: The effective phacoemulsification time in the standard Legacy group was under 0.4 min in 40 cases (20%), between 0.4-1.2 min in 120 cases (60%), and more than 1.2 min in 40 cases (20%). The effective phaco time in the AdvanTec-NeoSoniX group was under 0.4 min in 118 cases (59%), between 0.4-1.2 min in 72 cases (36%) and more than 1.2 min in 10 cases (5%). The mean phacoemulsification power varied between 19-38% in the first group and 14-22% in the second group. Descemetic striae and corneal edema on the first postoperative day were observed in 38 eyes (19%) in the Legacy standard group, and 18 eyes (9%) in the AvanTec-NeoSonix group. CONCLUSIONS: The progression from the standard Legacy to the AdvanTec Legacy system with NeoSoniX handpiece led to the reduction in phacoemulsification power by two mechanisms, oscillation, which breaks the nucleus, and vacuum, which removes and evacuates the nuclear material.

Aged↗

PubMed2004

[Phacoemulsification--personal experience on my first 507 cases].

PURPOSE: The evaluation of the intra and post operative early complications of the phacoemulsification with posterior chamber artificial lens implant on my first 507 cases. METHODS: A retrospective study on 507 eyes operated by cataract through phacoemulsification technique at the Emergency Military Hospital from Cluj-Napoca during the 1st of May-30th of June 2002. The surgeries were performed by the same surgeon through a corneal or a scleral incision using rigid and foldable artificial lenses. The evaluation period lasted 2 months, the types and frequency of the intraoperative and early postoperative complications being analyzed. RESULTS: The intraoperative complications were represented by the posterior capsule's efraction in 17 cases (3.37%); zonular rupture in 3 cases (0.59%); the loss of the nucleus into vitreous 4 cases (0.79%); the luxation of the crystalline lens in the anterior chamber in 5 cases (0.99%); the hyphaema and iris trauma in 14 cases (2.76%); incomplete cortical clean-up in 9 cases (1.78%); the failed capsulorhexis in 7 cases (1.38%) and the conversion to ECCE in 8 cases (1.58%). The early postoperative complications met into the study were: the corneal edema in 34 cases (6.71%); raised IOP in 18 cases (3.55%); the retained lens material in 9 cases (1.78%); the late inflammatory reactions in 6 cases (1.18%); hypopyon in 3 cases (0.59%); endophthalmitis in 3 cases (0.59%); retinal detachment in 1 case (0.2%) and the anterior capsule fibrosis in 4 cases (0.79%). CONCLUSIONS: The phacoemulsification is a high security technique because of the small incision and the sharp accuracy. The complications correlate themselves with the surgeon experience and with some particularities of the cataract (the brunescent cataract at the old patient, the medium dilatation of the pupil, etc.).

Follow-Up Studies↗

PubMed2004

[Phacoemulsification in white cataract].

PURPOSE: The study presents the technical difficulties of the capsulorhexis and phacoemulsification in the white, intumescent cataract and the incidences occurred during the foldable IOL implantation with the Monarch II injector. METHODS: The clinical study comprises a number of 70 eyes with white intumescent cataract operated during 15 January-15 December 2003 by the same surgeon. The surgical intervention consisted of lens extraction by phacoemulsification with clear corneal incision, using the Monarch II injector for the foldable IOL implantation. In 56 eyes, the anterior capsule was stained with trypan blue. RESULTS: The intraoperative complications include: failed capsulorexis in 4 cases (5.71%), rupture of posterior capsule in 1 case (1.43%) and the conversion to classical extracapsular extraction in 3 cases (4.29%). IOL implantation was made with the help of the Monarch II injector in 67 cases (95.71%). The foldable IOL implantation in the bag was realised a single time in 44 cases (62.9%). We had the following incidences in the other cases: the upside down turning of the IOL during unfolding in 2 cases, the second haptic capture in the tunnel of the cartridge in 2 cases, the second haptic stucks outside or in the anterior chamber in 18 cases, the first haptic stays in the sulcus and the second one stays outside in 1 case. CONCLUSIONS: In eyes with 1 intumescent cataract we can perform the continuous curvilinear capsulorhexis using trypan blue, but also without staining the anterior capsule using mechanical or manual aspiration of the liquefied cortex and viscoelastic substances. The foldable intraocular lens implantation with the injector proves to be an easy and comfortable method after it has been practiced for a longer period of time if the right size of the cartridge is used.

Capsulorhexis↗

PubMed2004

[Cataract surgery in biphakia patients].

INTRODUCTION: Although the IOL implantation was used initially just for cataract surgery, in the last period IOLs are used in treating myopia more than -12D and hyperopia more than +4D, in phakic eyes, when the indications of excimer laser are limited. OBSERVATION: We present the case of a 40 years old patient with the diagnosis: biphakia, complicated cataract and high myopia in both eyes. VA-RE = 2/50 cc, VA-LE = 3/50 cc, IOP-RE:15 mmHg, IOP-LE:16 mmHg. We performed, in two separate surgeries, the explantation of the intraocular lens, then the extraction of the opacified crystalline lens through the same incision and the implantation of an AcrySof foldable lens with an adequate power, in the bag. We discuss the causes of cataract formation in eyes with posterior chamber phakic intraocular lens, the choice of the IOL power, the incision for explantation and implantation, and the intraoperative technical difficulties. CONCLUSIONS: The association of phakic IOLs and cataract represents a new challenge to the ophthalmic surgeon, taking into account that these IOLs are becoming more and more popular to correct high refractive errors.

Adult↗

PubMed2004

[Phacoemulsification and intraocular lens implantation in myopic eye].

UNLABELLED: The purpose of this study is to estimate the technical difficulties and the intraoperative complications which appear in patients with high myopia who had been operated on cataract through phacoemulsification technique; to establish the IOL refractive power and to estimate the functional results at 45 days postoperative. MATERIAL AND METHOD: We performed a study on 44 eyes with high myopia and cataract operated in the Emergency Military Hospital from Cluj-Napoca during 15th of January -15th of June 2003. We analyzed the patients' age, the technical difficulties and intraoperative complications, the IOLs choosing and the functional results. RESULTS: The greatest frequency of patients with high myopia and cataract met into the study was between 50-60 years old (43.18%). The intraoperative complications were represented by the posterior capsule's refraction without loss of vitreous in one case, zonular rupture in one case and one case with loss of the nucleus into vitreous. CONCLUSIONS: In patients with high myopia and cataract the surgical procedure solves both visual problem and refractive deficiency. For the myopic eye is important to use IOL with 6 mm, 6.5 mm diameter.

Adult↗

PubMed2004

[Phacoemulsification in posterior polar cataract].

PURPOSE: To evaluate the technical particularities of phacoemulsification and the intraoperative complications in posterior polar cataract. MATERIAL AND METHODS: Prospective study which comprises 10 eyes with posterior polar cataract (8 patients), operated on between May 2001 and April 2004 in the ophthalmology department of the Military Hospital Cluj-Napoca, by the same surgeon. Phacoemulsification with low parameters was performed in all cases, and acrylic and PMMA intraocular lenses were implanted in 9 eyes. RESULTS: The patients' age was between 24 and 80 years, with a mean age of 55.12 years. The majority of the patients presented bilateral posterior polar cataract (70%), but only 2 patients were operated on the both eyes in the analyzed period. The posterior capsule rupture occurred in 4 eyes (40%). A posterior residual plaque was attended in one case, but did not influence the short term visual acuity. The visual acuity of the 3 cases which were affected unilaterally was not influenced by the surgery, which was without complications in 2 cases. The causes were preexisting amblyopia. CONCLUSIONS: The study confirms the high risk to posterior capsule rupture during posterior cataract surgery and the high incidence of the bilateral form (70%).

Adult↗