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Biomedical subjects

Diane Jones

Publications and source records attributed to Diane Jones.

8 recordsLinked to original sources
PubMed2006

You won't know if you're improving unless you measure: recommendations for evaluating Hospice-Veteran Partnerships.

Growing evidence indicates that there are abundant opportunities to improve the care that patients receive near the end of life. Hospice care has been associated with improvements in these and other outcomes, but hospice is underused by most patient populations. Therefore, the Department of Veterans Affairs (VA) has made hospice access a priority in its plan to improve end-of-life care for all veterans. In addition to committing funding for hospice care, the VA has also established a national network of Hospice-Veteran Partnerships (HVPs) whose goal is to improve access to hospice for veterans. This article describes the results of a nationwide consensus project to develop measures of the success of HVPs and recommends key measures that should be used to track improvements and to identify opportunities for highly successful collaborative strategies.

Cooperative Behavior↗
PubMed2006

Polyester wax: a new embedding medium for the histopathologic study of human temporal bones.

BACKGROUND: Celloidin and paraffin are the two common embedding mediums used for histopathologic study of the human temporal bone by light microscopy. Although celloidin embedding permits excellent morphologic assessment, celloidin is difficult to remove, and there are significant restrictions on success with immunostaining. Embedding in paraffin allows immunostaining to be performed, but preservation of cellular detail within the membranous labyrinth is relatively poor. OBJECTIVES/HYPOTHESIS: Polyester wax is an embedding medium that has a low melting point (37 degrees C), is soluble in most organic solvents, is water tolerant, and sections easily. We hypothesized that embedding in polyester wax would permit good preservation of the morphology of the membranous labyrinth and, at the same time, allow the study of proteins by immunostaining. METHODS: Nine temporal bones from individuals aged 1 to 94 years removed 2 to 31 hours postmortem, from subjects who had no history of otologic disease, were used. The bones were fixed using 10% formalin, decalcified using EDTA, embedded in polyester wax, and serially sectioned at a thickness of 8 to 12 mum on a rotary microtome. The block and knife were cooled with frozen CO2 (dry ice) held in a funnel above the block. Sections were placed on glass slides coated with a solution of 1% fish gelatin and 1% bovine albumin, followed by staining of selected sections with hematoxylin and eosin (H&E). Immunostaining was also performed on selected sections using antibodies to 200 kD neurofilament and Na-K-ATPase. RESULTS: Polyester wax-embedded sections demonstrated good preservation of cellular detail of the organ of Corti and other structures of the membranous labyrinth, as well as the surrounding otic capsule. The protocol described in this paper was reliable and consistently yielded sections of good quality. Immunostaining was successful with both antibodies. CONCLUSION: The use of polyester wax as an embedding medium for human temporal bones offers the advantage of good preservation of morphology and ease of immunostaining. We anticipate that in the future, polyester wax embedding will also permit other molecular biologic assays on temporal bone sections such as the retrieval of nucleic acids and the study of proteins using mass spectrometry-based proteomic analysis.

Adolescent↗
PubMed2004

Understanding why women decline HIV testing.

BACKGROUND: The Department of Health (DH) set a target for HIV testing--uptake should be at least 90% by 31 December 2002 (Department of midwife Health, 2000), i the number of 80% decrease in n order to see a babies born with HIV (Tookey et al, 2002). As an exercise to measure Newham Healthcare Trust against this standard and review the antenatal care HIV-positive women received, the maternity unit undertook an audit. One in 160 pregnant women receiving antenatal care in the London Borough of Newham are HIV positive (Communicable Disease Screening Centre, 2003), according to the HIV anonymous screening programme, however the rate of transmission to the baby is virtually zero, when babies were followed up at birth, six months and one year Healthcare NH S Trust, 2003). Newham's HIV testing uptake ranges from 80% to 90% (with an average of 88%). A strategy for improving the uptake has been to explore why the 10% to 20% of women decline testing; hence the Trust underwent an audit to explore the reasons women gave when they declined testing. Audit findings Some 2138 forms were returned over a seven-month period, of which 328 (15%) were from women who declined HIV testing. Uptake within that period ranged from 74% to 90%. Ten new positive women were identified. The HIV-specialist midwife carries a caseload and is aware of all positive women, therefore was able to identify the new cases. The reasons given for decline ranged from cultural and religious beliefs to those who said they were not at risk. Practice implications Where women are aware of their HIV status, services can be put in place to support them through their pregnancy and life as a positive parent, equally important is the ability to minimise the risk of transmission to babies, therefore it is vital that women understand the purpose of early diagnosis through testing and its consequences. Midwives have a vital role to play in ensuring pregnant women receive adequate information to form their decision. If a woman chooses not to have the test, midwives should explore her reasons without prejudice. It is also recommended that women who decline testing are reoffered the HIV test at their subsequent antenatal visit, allowing them the opportunity to digest the information and discuss it with a partner. The purpose of the audit was to assess how the Trust would meet the DH target (90% uptake), as part of a review of the maternity HIV service.

AIDS Serodiagnosis↗
PubMed2003

Do information systems meet the needs of primary care trusts?

To function effectively, primary care trusts (PCTs) need information from a range of sources. The general practice clinical record is a key source of information for PCTs but has often proved to be of variable quality. PCTs have developed rapidly and now have responsibility for 75% of the healthcare budget. They have a range of information needs that must be met if they are to ensure that healthcare provision meets the needs of patients. Since the abolition of health authorities PCTs have full responsibility for developing practice systems as well as having a key role to play in developing information management and technology (IM&T) within local health economies. The paper describes the problems PCTs face in accessing information to support their core functions and the progress they have made in retrieving data to support service development. It also describes the progress and developments in information and information systems within general practice using data from the National Tracker Survey of Primary Care Groups and Trusts (PCG/Ts). The survey began in 1999 when PCGs were established and has tracked the progress of PCG/Ts over three years. This paper presents the findings from the third survey relating to the development of information to support the needs of PCG/Ts. The findings show that information available to PCG/Ts does not meet their needs in a range of key areas. Many PCG/Ts are collecting data to support the Coronary Heart Disease National Service Framework (CHD NSF) and monitoring some areas of service provision in general practice. The use of information management tools has risen significantly since the second survey and involvement in the Primary Care Information Services (PRIMIS) initiative has more than doubled. The paper concludes that although PCG/Ts and general practices have made substantial progress, there is a long way to go before information providers generate high-quality information to support the needs of PCTs.

Attitude of Health Personnel↗
PubMed2003

Overcoming barriers to better care.

Pharmacological developments and the shift of emphasis to primary care are just two developments that make it possible to widen access to cardiac services to more patients.

Community Health Services↗