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Dianna G Goodall

Publications and source records attributed to Dianna G Goodall.

2 recordsLinked to original sources

Terraced self assembled nano-structures from laminarin.

The formation of self assembled nano-structures from the biopolymer laminarin dried onto mica is reported. The observed structures are composed of stacked terraced layers decreasing in size away from the mica surface. The layers display a high degree of dimensional regularity as observed using atomic force microscope imaging (AFM). The width of the layers is linearly dependent upon the number of layers in the structure and decreases with layer number away from the mica substrate. The thickness of the layers is uniform throughout the structure. A pore is contained in the central region of each structure with more than one layer. We postulate that these structures have potential use as templates in microelectronic devices and sensors where the central pore has the potential to immobilise functional materials.

Aluminum Silicates↗

Shear flow induces amyloid fibril formation.

Shear flow is indirectly implicated in amyloid formation in vitro. Despite the association between amyloid fibrils and disease, and the prevalence of flow in physiological systems, the effect of this parameter is uncharacterized. We designed a novel Couette cell to quantitatively investigate shear exposure during fibrillogenesis. Amyloid formation by beta-lactoglobulin was monitored in situ with real-time fluorescence measurements across a range of shear rates. We demonstrate shear-induced aggregation of spheroidal seed-like species. These seeds enhance fibril formation in native beta-lactoglobulin, thereby demonstrating that shear flow generates an amyloidogenic precursor. Furthermore, preformed fibrils are degraded by exposure to high shear rates. Our results have implications for the mechanism of amyloid formation in physiological flow conditions.

Amyloid↗