Biomedical subjects

Dong-Feng Gu

Publications and source records attributed to Dong-Feng Gu.

7 recordsLinked to original sources

PubMed2006

Common SNPs of APM1 gene are not associated with hypertension or obesity in Chinese population.

OBJECTIVE: To investigate whether the common variants 45T/G and 276G/T in APM1 gene were associated with hypertension combined with obesity (HO) and related clinical features in Chinese Han population. METHODS: A case-control study design was applied. Common polymorphisms of 45T/G and 276G/T were genotyped by PCR product sequencing in 484 cases with HO and 502 controls with normal blood presure and BMI < 25. RESULTS: The genotype and allele frequencies of 45T/G, 276G/T, and haplotype defined by the two variants in cases did not differ from those in controls. The means of blood pressure, BMI and waist-hip ratio did not differ among genotypes of the two polymorphisms and haplotypes. Among lipid profiles, only serum high-density lipoprotein cholesterol (HDL-C) levels were significantly lower in T allele carriers than that in non-T carriers after adjusting possible confounding factors (1.21 vs 1.32 mmol/L, P=0.0001). CONCLUSION: Polymorphisms of 45T/G and 276G/T in APM1 gene are not associated with hypertension or obesity, or their clinical features in Chinese Han population. Common polymorphism of 45T/G might be associated with serum HDL-C levels in Chinese.

Adiponectin↗

PubMed2005

[Association analysis between polymorphisms of PON gene cluster with coronary heart disease in Chinese].

An extensive association analysis of PON gene cluster (PONs) with coronary heart disease (CHD) was performed in Chinese Han population. Eleven polymorphisms of PON1, PON2 and PON3 gene were investigated for association with CHD in 474 male patients and 475 controls. Univariate analyses showed the cases had significantly higher frequencies of PON1 192Q allele, 160R allele, -162A allele and PON2 311C allele than were seen in the controls. Logistic regression analyses revealed only the PON1 R160G and -162G/A polymorphisms remained significantly associated with CHD (P = 0.0054 and P = 0.0002). Haplotype analyses for various polymorphism combinations further confirmed the results of individual polymorphism analyses. Only the frequencies of haplotypes containing -162A allele were significantly higher,whereas only the frequencies of haplotypes containing 160G allele significantly lower in cases than those in controls in various polymorphism combinations. This extensive association study has identified the PON1 -162G/A and R160G polymorphisms to be independently associated with CHD in Chinese Han population,and warrants further study to elucidate the biological mechanism.

Adult↗

PubMed2005

[Polymorphisms screening of PON gene cluster].

To identify all putative functional polymorphisms of PON gene cluster in Chinese Han population. Common polymorphisms of PON1, PON2 and PON3 gene were identified by directly sequencing of genomic DNAs derived from 48 randomly selected patients with coronary heart disease. We designed PCR arrays to amplify regions up to about 1kb upstream from transcription-initiation sites, i.e., putative promoter regions, all exons and adjacent non-coding regions. In a total length of 13.9 kb explored, we identified thirty-one SNPs, of which, 17 were first reported. A new coding polymorphism was detected in PON1 gene, which gives rise to amino acid substitutions of arginine (R) for glycine (G) at codon 160, whereas L54M polymorphism, which is common in white population, was not detected in our Han population. Among the five polymorphisms identified in PON3 gene, one in the promoter regions at position -133 (C/A) was located in a potential binding site for transcription factor LF-A1. Allele frequencies of some polymorphisms are significantly different from those reported in Caucasian populations. Complete or nearly complete association between polymorphisms was frequently observed. The identified multiple putative functional polymorphisms in PON gene cluster and their linkage disequilibrium patterns in combination with the population specific frequencies are of values for futher association studies of PON gene cluster with cardiovascular disease.

Aryldialkylphosphatase↗

PubMed2004

[Pulse pressure and prevalence of stroke and myocardial infarction in Chinese population].

OBJECTIVE: To explore the characteristics of distribution of pulse pressure (PP) in Chinese population and its relationship to major cardiovascular diseases in them. METHODS: Data from the Third National Blood Pressure Survey involved 882,681 subjects aged over 18 in 1991 were reanalysed for the relationship between PP and risks of major cardiovascular diseases, with t-test, chi2 test and logistic regression model. PP is defined as the difference between systolic and diastolic blood pressure. RESULTS: (1) Overall mean PP was (44.61 +/- 13.59) mm Hg for the subjects aged over 18, higher in men [(44.92 +/- 12.72) mmHg] than that in women [(44.34 +/- 14.32) mmHg]. PP increased progressively with age, and its increase accelerated significantly at age over 50 but higher in women than in men. Proportion of the subjects with PP equal to or greater than 60 mmHg was more in those aged 60 or over than that in younger ones. (2) Prevalence of stroke and myocardial infarction (MI) increased with PP and age, whether in the normotensives, or in the hypertensives or in the isolated systolic hypertensives. (3) Results of multivariate logistic regression analysis revealed that risks of stroke in the subjects with PP of (45-59) mmHg, (60-74) mmHg and over 75 mmHg were 1.9, 3.5 and 5 times as in those with PP less than 45 mm Hg, respectively, adjusted for other risk factors, and their risks of myocardial infarction (MI) were 1.2, 1.5 and 1.7 times, respectively. Furthermore, PP was significantly and independently related to the risks of stroke and MI, even adjusted for systolic and diastolic pressures. CONCLUSIONS: Prevalence of stroke and MI increased with the breadth of PP and age. Broader PP may be an important and independent predictor of risks of stroke and MI, especially in the aged people.

Adolescent↗

PubMed2004

[Issues on association studies on complex diseases].

Association study is widely used in elucidating genetic basis of complex diseases such as cardiovascular diseases, type 2 diabetes, essential hypertension and obesity. Some issues were discussed in the review, which include defining and controlling of confounding effects, selection of candidate genes and single nucleotide polymorphisms (SNPs), application of intermediate phenotype and haplotype analyses as well as judgement of the result in association studies. Population stratification is one of the major causes of confounding in association studies. It could be reduced by selecting relatives of affected patients as control, genome control and by using isolated populations which have higher homogeneity in genetic background. A candidate gene could be selected because of its' biological association with some disease or being congenerous to a known gene related to the disease. Appropriate application of intermediate phenotype of diseases and haplotype analyses may increase the opportunity to obtain meaningful findings in association studies. An optimal study design, sufficient sample size and proper controls, in conjunction of modern statistic analyses, association analyses would exert its effect on studies on susceptibility of human common diseases.

Cardiovascular Diseases↗

PubMed2004

[Two approaches of quantitative-trait linkage analysis].

ln this article, we discussed two model-free methods for detecting genetic linkage for quantitative traits, Haseman-Elston regression approach and variance components approach. The former is a regression approach for detecting linkage based on the squared difference or squared sums in quantitative trait values of sib-pairs and their estimated marker IBD scores. The latter can jointly model covariate effects along with variance components, including genetic component and non-genetic sources of variability. We have outlined the model assumption, the algorithm and the extensions for the both methods.

Computer Simulation↗

PubMed2002

[The insertion/deletion polymorphism of angiotensin I converting enzyme gene and coronary heart disease--discordant sib pair analysis and transmission/disequilibrium test].

To investigate whether the insertion/deletion polymorphism of the human angiotensin I converting enzyme gene increased the risk of coronary heart disease (CHD) in CHD pedigrees,discordant sib pair analysis (DSP) and transmission/disequilibrium test (TDT) were used. Forty-five CHD pedigrees with at least one CHD patient in the first degree relatives of probands were recruited during Oct. 1998 to Feb. 1999, of which parental genotype known, one or both parental genotype missing was 21.2 and 22 respectively. ACE genotype was measured by PCR technique. Conditional Logistic regression was used to analyze the DSP, and TDT-STDT program 1.1 was used for TDT and STDT. Univariable conditional Logistic regression did not find significant difference of the distribution of three different ACE genotypes in the 106 discordant sib pairs obtained from the 45 pedigrees. After adjusting effects of traditional risk factors of CHD, no significant difference of the distribution was found by multiple Logistic regression model. Neither the TDT for 13 nuclear families or STDT (sib transmission/disequilibrium test) for 24 sibships showed significant difference between the transmitted and untransmitted ACE gene D allele distributions. Our results show that the insertion/deletion polymorphism of ACE gene is not associated or linked with CHD in Chinese population.

English Abstract↗