Biomedical subjects

Dong-Po Zhou

Publications and source records attributed to Dong-Po Zhou.

3 recordsLinked to original sources

PubMed2005

[Differential display technique and its progress].

Differential display technique is an important method to isolate differentially expressed gene. Comparing to other methods like representational difference analysis, suppression subtractive hybridization and serial analysis of gene expression, differential display technique is used in higher frequency. Since it was established in 1992, it has overcome many disadvantages and widened its practical fields through improvements and enhancements by global researchers. In this paper the principle and the main advantages and disadvantages of differential display technique were briefly introduced. Meanwhile, the four progressed aspects in designing primer, reducing false positives, identifying differentially expressed gene and techniques derived from DD were introduced in detail.

Blotting, Northern↗

PubMed2005

[Study on breeding up high-yield strain of taxol by protoplast mutagensis].

In order to obtain resistant mutants to nystatin, ultraviolet radiation and LiCl were used to mutagenize the protoplasts of taxol-producing fungi NCEU-1, and four positive mutants with high yield of taxol were screened out on nystatin flat. After further screening experiments on fermentation, a mutant strain--UL04-5 which was able to produce taxol with high yield and could be stably passed on in genetics was eventually found, it's ability to produce taxol was improved from 314.07 microg/L (strain NCEU-1) to 418.24 microg/L (strain U04-5).

Ascomycota↗

PubMed2005

[Study on effect of Lactobacillus acidophilus MG2-1 on serum lipid metabolism in rats].

Wistar rats were fed with a high lipid diet supplemented with living or thermal death bacteria of Lactobacillus acidophilus MG2-1 which was isolated from koumiss in Mongolia and was of good ability of acid tolerance and decreasing the level of cholesterol in vitro. The effect of Lb. acidophilus MG2-1 on the metabolism of serum cholesterol was discussed. It was showed that it was on the 14th day of experiment that the inhibiting effects of the increase of serum cholesterol level of rat groups fed with living bacteria and heat-killed bacteria was significantly (p > 0.05) and very significantly (p < 0.01) higher than that of the high lipid diet group respectively; at the same time, the level of serum HDL-C of the thermal death bacteria group was significantly higher than that of the high lipid diet group (p < 0.05), also arteriosclerosis index of wistar rats in experimental group is significantly lower than that of the high lipid diet group (p < 0.01). The total bile acid level of the thermal death bacteria group in fecal is significantly higher than that of the high lipid diet group (p < 0.05). It is suggested that the increase of serum cholesterol level in rats can be inhibited and arteriosclerosis can also be prevented by this strain. During the period of tests, the effect of the strain on serum lipid in rats weaken with the time going, while the dose of bacteria fed was not changed.

Animals↗