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Biomedical subjects

E A Calder

Publications and source records attributed to E A Calder.

16 recordsLinked to original sources

Screening for cancer and coronary risk factors through a nurse practitioner-staffed preventive health clinic.

The Preventive Health Examination (PHE) program was designed to screen for cancer of the lung, colon, skin, and prostate (or breast) and to detect the risk factors for coronary artery disease, i.e., arterial hypertension, hypercholesterolemia, cigarette smoking, and obesity. To investigate the cost-effectiveness of PHE performed by nurse practitioners, the first 176 subjects enrolled in the PHE program at a northeastern, suburban VA Medical Center were evaluated prospectively. The subjects were recruited through random mailing. The direct cost of PHEs was $80 per patient. PHEs were performed on 171 men and 5 women, mean age 57.2 years, divided into groups according to the date of evaluation. Nine percent of patients had findings highly suspicious of cancer (lung nodules in 7, skin lesions in 9). As a consequence, one patient underwent lobectomy for squamous carcinoma of the lung and another underwent prostatectomy for adenocarcinoma of the prostate. Fifty-five percent of patients had high cholesterol levels, 53% were obese, 30% were heavy cigarette smokers, and 19% were hypertensive. Nineteen percent of the patients had three or more coronary artery disease risk factors. We conclude that low cost PHEs performed by nurse practitioners have a high yield in detecting risk factors for coronary artery disease, and for detecting potentially treatable malignancies.

Adult↗

T, B and K cells in autoimmune thyroid disease.

The K-cell cytotoxic activity of peripheral blood lymphoid cells from 104 patients with autoimmune thyroid disease and from age and sex matched control subjects was measured using chicken erythrocytes as target cells. Patients with Hashimoto thyroiditis,primary hypothyroidism and thyrotoxicosis who were either newly diagnosed and untreated or had received therapy for less than or equal to 1 year showed a significant increase in K-cell cytotoxic activity. Patients who had received treatment for greater than 1 year and less than or equal to 5 years showed no such comparable increase in cytotoxic activity. Within the group of patients with untreated thyrotoxicosis it was found that K-cell cytotoxic activity was related to both goitre size and serum antibody titre. Thus patients with little or no goitre showed a highly significant elevation of cytotoxic activity whereas patients with moderate to large goitres gave values within the normal range. Similarly patients with no detectable serum thyroid autoantibodies showed high K-cell activity while patients with positive antibody titres did not. It was also shown that neither the absolute number nor the proportion of circulating T and B lymphocytes in patients with autoimmune thyroid disease as assessed by the sheep red cell rosette method and by indirect immunofluorescence was significantly different from that observed in the normal control population. No correlation was found between peripheral blood K-cell cytotoxic activity and the percentage of circulating null cells, i.e. 100-(percentage T + percentage B) in either patients or control subjects.

Adolescent↗

K cell cytotoxicity against antibody-coated chicken erythrocytes in tumor-bearing mice: its development with progressively growing tumor and the effect of immunization against the tumor.

Antibody-dependent (K cell) cytotoxic activity of spleen cells from mice bearing a chemically induced fibrosarcoma has been studied by using antibody-coated chicken erythrocytes as target cells. Spleen cells from tumor-bearing animals caused a significantly greater degree of target cells destruction than did those from control animals. The elevated cytotoxic activity in tumor-bearing animals increased with time after the tumor inoculation and correlated directly with the size of the tumor. The development of increased cytotoxic activity could be circumvented by surgical removal of the tumor. Mice that received x-irradiated tumor cells of x-irradiated tumor cells followed by a live challenge did not show a tumor growth and also failed to show increased K cell cytotoxic activity. It has been concluded that the increased K cell activity results directly from the active growth of tumor. The role of K cells in immunosurveillance has been discussed.

Animals↗

K-cell cytotoxic activity in the spleen and lymph nodes of tumour-bearing mice.

The cytotoxic activity of spleen and lymph node cells from female CBA mice bearing a methylcholanthrene-induced fibrosarcoma on target cells coated with anti-target cell antibody has been investigated. Spleen cells from tumour-bearing mice caused a significantly greater degree of target cell lysis than did spleen cells from control mice. This increase in cytotoxicity was most apparent when a ratio of ten lymphoid cells to one target cell was used. With control mice, the mean cytotoxic index was 13.54 +/- 1.05 (s.e.m.) as compared to 55.00 +/- 6.11 with tumour-bearing mice. An increase in the cytotoxic activity of lymph node cells was also noted. In control mice, the cytotoxic index, using a ratio of 100:1 was 8.55 +/- 2.27. In tumour-bearing mice, the mean cytotoxic activity of the draining lymph node was 21.66 +/- 4.96, and of the contralateral lymph node, 13.00 +/- 3.46.

Animals↗

The effect of anti-alpha2-macroglobulin on K-cell cytolysis and T- and B-cell rosette formation.

The effect of antisera to human alpha2-macroglobulin (alpha2M) on the ability of human peripheral blood lymphoid cells to lyse antibody coated target cells (exert a K-cell effect) and form T(E) and B(EAC) rosettes has been investigated. Pretreatment of lymphoid cells with these antisera inhibited their K-cell activity but had no effect on their capacity to form T or B rosettes. The inhibitory activity of the anti-alpha2M sera was always reduced following absorption on a CnBr column to which human alpha2M had been coupled. The absorbed antibody, recovered by acid elution reacted strongly with human alpha2M in gel diffusion precipitin analysis and caused a highly significant inhibition of K-cell cytotoxicity. It was further shown that the inhibitory activity of the anti-alpha2M was localized exclusively in the IgG fraction and was greatly reduced following pepsin digestion.

Antibodies↗

Evidence for circulating immune complexes in thyroid disease.

Anti-complementary activity was detected in the sera of 23 (59%) out of 39 patients with Hashimoto thyroiditis; in 10 (29%) out of 34 patients with primary hypothyroidism; in six (17%) out of 36 patients with thyrotoxicosis; and in only six (8%) out of 78 control subjects. Fractionation of Hashimoto sera by Sephadex G-200 chromatography showed that the anti-complementary activity was founding fractions of larger molecular size than monomeric IgG, being localized predominantly in the ascending limb of the second elution peak, suggesting that it was in the form of small immune complexes.

Animals↗