Biomedical subjects

E A Chernen'kaia

Publications and source records attributed to E A Chernen'kaia.

3 recordsLinked to original sources

PubMed1989

[Recombinant plasmids containing hybrid protein genes with antigenic determinants of the foot and mouth virus].

Plasmids have been constructed which contain genes coding for fused proteins including beta-galactosidase or human leukocyte interferon alpha 2 and monomeric or pentameric form of the main antigenic determinant of the foot-and-mouth disease virus (FMDV) serotype 01K. Expression of the hybrid genes has been studied. It is shown that fused proteins, containing beta-galactosidase and the antigenic determinant (monomer or pentamer), interact specifically with anti-FMDV anti-sera and with antibodies against peptide 141-160 of FMDV VP1 coat protein.

Amino Acid Sequence↗

PubMed1988

[Recombinations during DNA cloning].

RecA-independent recombinations accompanying the processes of plasmids preparation and cloning into Escherichia coli cells are induced within the short direct and inverted repeats of several types.

Base Sequence↗

PubMed1985

[New plasmid vectors for cloning and the expression of genes].

Multicopy plasmids of pMCR series have been constructed from pRRN2 (a pBR322 derivative) including plasmids carrying transcription terminators downstream the tet gene and those with opposite orientation of the tet and RNAI genes. The plasmids permit cloning and high expression of genes with strong promoters.

Chromosome Mapping↗