Biomedical subjects
Publications and source records attributed to E A Ignatova.
Addition of green tea extract in concentrations of 0.004-0.006% to the nutrient medium markedly stimulated the growth of spinal ganglion neurites of 1-2-day-old rats.
The effect of baikal skullcap extract on the development of dorsal root ganglia from 1-2-day-old rats in organotypic cultures was studied. Baikal skullcap extract produced a dose-dependent stimulating effect on neurite growth in neurons of dorsal root ganglia.
Effects of balis-2 on exploratory activity in the open field and elevated plus-maze, attention to sensory stimuli of different modalities, elaboration and retention of conditioned reflexes with food reinforcement, were studied in rats under stress. Prolonged treatment of rats with balis-2 seems to normalise integrative activity and metabolism of serotonine and dopamine in the animal brain.
Based on a study of 200 lactic-acid bacteria monocultures and 30 associating lactic bacteria and yeasts cultures, a stable association was created formed by Leuconostoc oenos, Pediococcus pentosaceus and Saccharomyces cerevisiae yeasts, intended for the biological deacidification of wine. Physiology of microorganisms and their effect on the wine chemical composition was studied. By means of selective association, high quality fine wines were produced from the high-acid wines.
Hemagglutinating properties of culture liquid and cells of 142 yeast strains of species Saccharomyces cerevisiae, Kluyveromyces marxianus, K. dobzhanskii, K. lactis var. lactis and K. lactis var. drosophilarum, isolated from various sources have been studied. A capacity of yeast (44% of the studied strains) to synthesize both lectins bound to a cell and extracellular lectins has been established; the latter have been found in yeast for the first time.
Life cycles of Candida utilis (Henneberg) Lodder et Kreger-van Rij imperfect yeast (13 strains) used in industry were the study subject. When the strains were mated, we detected one of the stages of sexual process--conjugation of cells of the opposite mating type. Most of the studied cultures conjugated on the 2d-3d day. No ascospores were formed. Haploidy and heterothallism of the studied C. utilis strains were confirmed by hybridization of auxotrophic mutants. Based on PCR assay results, the yeasts are demonstrated to belong to the ascospore perfect yeast species of Pichia jadinii (A. et R. Sartory. We ill et Meyer, Wickerham) Kurtzman.
Strains (103) of yeast from Pichia genus: P. anomala, P. guilliermondii and P. membranaefaciens species have been investigated. The researchers have found 87 strains (84.5%) capable to form extracellular and surface lectins with activity to 512 hemagglutinating units. The overwhelming number of strains (75.7-92.6%) synthesized lectins of the both forms. Considerably less quantity of lectin producers of some form--extracellular or surface ones--have been found. Availability of producers of extracellular lectins was characteristic to greater extent of the yeast of P. anomala and P. guilliermondii, and producers of surface lectins--mainly of P. guilliermondii. The most active producers of lectins have been isolated from human and animal organisms, soils and wine.
Yeast flora composition has been studied in natural populations of blood-sucking mosquitoes of genera Aedes. Anopheles and Culex dwelling in the territory of Ukraine. The authors have distinguished 18 yeast species belonging to 7 genera. Species Candida famata, C. (Yarrowia) lipolytica as well as the yeast of genera Rhodotorula and Cryptococcus dominate on the larvae and imago of mosquitoes. The distinguished strains are the components of normal microflora of blood-sucking mosquitoes and take no pathogenic effect on the laboratory culture of mosquitoes Aedes aegypti.
Quantitative and species compositions of yeast contaminating eggs, fry and fingerlings of Salmo gairdneri Rich under artificial breeding have been studied. Prevalence of species of genera Candida, Rhodotorula, Cryptococcus and Debaryomyces is noted. Yeast isolated from perished eggs and sick fry do not possess pathogenic properties. Certain strains of yeast make stimulating effect on the studied microorganisms.