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Biomedical subjects

E A Rowe

Publications and source records attributed to E A Rowe.

28 records · Page 2Linked to original sources
PubMed1980

Impaired lipogenesis in mammary glands of lactating rats fed on a cafeteria diet. Reversal of inhibition of glucose metabolism in vitro by insulin.

In lactating rats fed on a cafeteria diet (chow plus palatable high-energy foods) the decreased glucose uptake and lipogenesis in vitro in acini correlated with the depressed mammary-gland lipogenesis in vivo. Insulin in vitro restored the rate of glucose uptake and its conversion to lipid to values approaching those for acini from rats fed on the chow diet alone.

Animals↗
PubMed1980

Persistent obesity in rats following a period of consumption of a mixed, high energy diet.

1. Adult male hooded rats which were offered a mixed, high energy diet for 90 days were hyperphagic and became significantly obese compared to chow-fed control rats. Fasting plasma insulin and glucose levels were initially elevated in the experimental rats, but later in the 90 day period were similar to control levels. 2. When the high energy foods were withdrawn after 90 days and just chow was available, the obese rats maintained the elevated body weights. The obese rats were initially hypophagic, but chow intakes rapidly reached control levels. Plasma insulin and glucose levels were similar in both groups, suggesting that the persisting obesity may not be associated with altered insulin resistance. 3. Five weeks after withdrawal of the 'fattening' diet, half of the experimental rats were offered restricted access to chow for 27 days to reduce their weights to control levels. When the rats were again given free access to chow, they returned to the previously elevated weight. 4. Eighteen weeks after withdrawal of the 'fattening' diet, the experimental rats had significantly elevated body weights and fat stores. The elevated body weight was not simply due to increased growth because, although the experimental rats had slightly more lean body mass than the control rats, the increase in fat was not related to body size.

Animals↗
PubMed1979

Bombesin suppresses feeding in rats.

Bombesin (BBS) is a tetradecapeptide originally isolated from amphibian skin1. BBS-like immunoactivity is widely distributed in mammalian gut2-5, and plasma levels have been shown to rise sharply following feeding (ref. 6 and V. Erspamer, personal communication). The physiological actions of BBS are unknown. We have previously shown that the classic gut hormone cholecystokinin (CCK) is a powerful and specific suppressor of food intake7-9. Although CCK and BBS lack common amino acid sequences, they have certain common actions on gut viscera10,11. We have now shown that BBS also suppresses food intake, and we compare its action with that of CCK.

Animals↗
PubMed1979

Plasma pentazocine radioimmunoassay.

A sensitive and specific radioimmunoassay of dog and human plasma pentazocine is described. Rabbit antiserum and the second antibody method separated bound from free pentazocine. The radioimmunoassay employed an 125I-labeled radioligand and required extraction from the sample prior to quantitation. The method had a detection limit of approximately 200 pg/assay tube (1 ng/ml). The assay was used successfully to measure pentazocine in the plasma of beagle hounds given 0.3 mg of pentazocine/kg iv. The decline in plasma levels fitted a two-compartment body model with a 100-min mean overall half-life and a 3.2-liters/hr mean plasma clearance rate.

Animals↗
PubMedDate not supplied

Analysis of iosulamide in plasma and urine: application to intravenous pharmacokinetics in rhesus monkey.

A sensitive, specific, high-performance liquid-chromatographic method for the determination of iosulamide in plasma and urine is described. The method was used to determine pharmacokinetic parameters of iosulamide after intravenous administration to rhesus monkeys. The mean (+/- SE) distribution and disposition half-lives were 0.19 (+/- 0.03) and 1.5 (+/- 0.3) hr, respectively. The mean (+/- SE) model-dependent and model-independent volumes of distribution at steady state were 0.41 +/- (0.078) and 0.49 (+/- 0.039) liters/kg, respectively. Total urinary excretion of iosulamide represented a mean (+/- SE) of 12.5 (+/- 0.6)% of the administered dose and was virtually complete in 3 hr. The results of the pharmacokinetic study indicate that iosulamide is rapidly cleared from the body and that renal clearance is a minor route of elimination from the body.

Animals↗