[Evaluation of platelet metabolism in normal conditions and ischemic heart disease].
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Adult↗
Biomedical subjects
E A Savchenko
Publications and source records attributed to E A Savchenko.
Culturing of specialized glial cells (olfactory ensheathing cells) of human olfactory epithelium.
A monolayer of dissociated glial cells of human olfactory epithelium was cultured in Petri dishes and 12-well plates using a polylysine-laminin substrate. Primary cultures were subcultured after 10-15 days. The cell cultures were analyzed by phase contrast microscopy at all stages of culturing. A cytological study involved histological methods (trypan blue staining) and immunocytochemical visualization of GFAP, nestin, and low-affinity nerve growth factor receptors. At the final stage of culturing (5 passages) the monolayer cultures included 2 types of cells: GFAP- and p75-positive glial cells and nestin-positive fibroblasts.
Cells, Cultured↗
[An efficiency study of transplantation of embryonic nervous-tissue preparations in experimental 6-OHDA-induced hemiparkinsonism].
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Animals↗
Comparison of the efficacy of cell preparations from embryonic ventral mesencephalon of various prenatal age transplanted intrastriatally to rats with 6-OHDA-induced Parkinsonism.
Cell preparations of ventral mesencephalon obtained from 8-, 14-, and 16-17-day rat embryos were stereotactically transplanted to homologous rats with 6-hydroxydopamine-induced hemiparkinsonism. Automated analysis of apomorphine-induced motor asymmetry for 3 months after neurotransplantation revealed higher efficacy of cell preparations from 8- and lower from 16-17-day-old embryos. These data correlated with histomorphological findings, in particular, with the size of grafts, glial reaction, and the number of dopaminergic neurons in the grafts.
Animals↗
[Possibilities of magnetic resonance tomography in pancreatic cancer diagnosis].
There was conducted examination of 27 patients with pancreatic cancer using clinico-laboratory and instrumental methods: ultrasonic investigation, computeric tomography (CT), magnetic-resonance tomography (MRT). MRT was uninformative when pancreatic tumor was up to 3 cm in diameter. In diagnosis of cystadenocarcinoma, affection of common biliary duct and vessels and in small focal metastatic hepatic affection the results of MRT showed enhanced precision in comparison with CT. Informativity of MRT and CT in diagnosis of metastases in lymph nodes was equal.
Adenocarcinoma↗
Immunoenzyme assay of glial fibrillary acidic protein for evaluation of functional activity of cell grafts from embryonic ventral mesencephalon in rats with experimental hemiparkinsonism.
The relationship between the release of glial fibrillary acidic protein (GFAP) into systemic circulation and the efficacy of transplantation of embryonic nervous tissue was studied on rats with 6-OHDA-induced hemiparkinsonism. It was found that intrastriatal transplantation of cell preparations from embryonic ventral mesencephalon significantly attenuated apomorphine-induced rotation, which points to functional recovery of the dopaminergic nigrostriatal system. The degree of this recovery depends on reactive astrogliosis around the graft and survival of dopaminergic neurons. Analysis of GFAP concentration revealed significant elimination of this antigen into the circulation 7 and 14 days after transplantation. In rats with good consolidation of the graft without pronounced reactive gliosis, the concentration of GFAP reached 253.99+/-79.30 ng/ml on week 4 after transplantation and decreased to 8.2+/-3.3 ng/ml 8-12 weeks after transplantation. In rats with poor graft consolidation associated with death of transplanted neurons and gliosis in the graft and surrounding tissue the concentration GFAP increased to 476.4+/-111.0 ng/ml within 4 weeks after transplantation and remained elevated (235.0+/-44.8 ng/ml) for 12 weeks. Thus, monitoring of serum GFAP concentrations allows in vivo evaluation of the functional state of intracerebral graft and the level of reactive gliosis. This test can be used for the prognosis of transplantation efficacy.
Animals↗
Catecholamines and their metabolites in the brain and urine of rats with experimental Parkinson's disease.
The content of catecholamines and their metabolites in the brain and the relationship between cerebral catecholamine levels and their urinary excretion were studied in rats with 6-OHDA-induced hemiparkinsonism. 6-OHDA reduced brain concentrations of dopamine, DOPAC, and homovanilic acid and urinary excretion of dopamine, dioxyphenilalanine, and DOPAC by more than 90%. A positive correlation was found between the concentrations of these metabolites in the urine and striatum. Measurement of urinary catecholamines and their metabolites is a perspective test for evaluating the status of the dopaminergic nigrosostriate system of the brain in experimental parkinsonism.
3,4-Dihydroxyphenylacetic Acid↗
Complex analysis of efficiency of transplantation of embryonic nerve tissue to rats with hemiparkinsonism.
Effect of transplantation of embryonic ventral mesencephalon preparation containing dopaminergic neurons on repair of the dopaminergic nigrostriatal system was studied in rats with hemiparkinsonism induced by 6-hydroxydopamine. Transplantation of embryonic ventral mesencephalon into denervated striatum led to a more than 50% decrease in apomorphine-induced rotation, recovery of dopamine and DOPAC levels in the brain, and to an increase in DOPAC excretion and the DOPAC-dopamine ratio in daily urine of rats with hemiparkinsonism. Dopaminergic neurons of the transplant survived, forming a network of tyrosine hydroxylase-positive processes growing beyond the transplant and reinnervating the adjacent compartments of the striatum. A positive correlation between urinary excretion of DOPAC and brain concentration of dopamine was revealed in denervated rats after transplantation of ventral mesencephalon. Intrastriatal transplantation of cell preparations of embryonic striatum containing no dopaminergic neurons and isolated local injury to the striatum did not affect regeneration of the denervated nigrostratal system.
3,4-Dihydroxyphenylacetic Acid↗
[Possibilities of x-ray methods in diagnosis of an acute pancreatitis and its local complications].
The ray methods of investigation were applied in 182 patients with an acute pancreatitis (AP). In 28% of patients local complications of AP were revealed: pseudocyst, abscess, pancreatic gland phlegmon, the gastric wall infiltration and ulceration, paracolic infiltrate and colonic obstruction, biliary hypertension, portal hypertension, subcapsular and parenchymatous splenic hematoma.
Acute Disease↗
[Effects of laser therapy in patients with functional class II-IV effort angina (using instrumental techniques of the study)].
Infrared laser therapy using an arsenide-gallium laser was used in 54 patients aged 30-75 years who suffered from coronary heart disease. A positive clinical effect was achieved in 47 (87%) of 54 patients, as evidenced by the reduced number of nitroglycerin tablets used and improved bicycle ergometric, echo- and electrocardiographic parameters in the patients.
Adult↗
[Chronotropic insufficiency in patients with ischemic heart disease].
A study is presented of 80 patients with ischemic heart disease (IHD): 37 patients with a low reaction of the cardiac rhythm to physical load--chronotropic insufficiency (CHI) and 43 patients with an adequate reaction of the cardiac rhythm to loads. Physical working capacity was evaluated by the strength of threshold load, pulsepressure double product, rest quotient, oxygen pulse, oxygen consumption per 1 kgm of work. Interval between the 1 and 2 examination--6.5 years. It was shown that CHI in IHD does not influence the prognosis of the disease and does not reflect the grade of its severity. They are, however, the marker of involvement of the atria. Chronic insufficiency in ischemic heart disease makes it difficult to objectively evaluate the physical working capacity.
Coronary Disease↗
[Teaching experiment in the evaluation of the choice of methods in postgraduate education of radiologists].
A pedagogical experiment was used to study the effectiveness of a system of program-purpose teaching in postgraduate training of radiologists differing from the classic methods aimed at end goals. A total of 150 pedagogical tests on 240 students using parallel and cross methods were performed. The use of the system of program-purpose teaching intensifies the physician's activities, increases the effectiveness of acquiring professional habits and their retention.
Education, Medical, Graduate↗
[Surgical treatment of dysplasia of the dorsal artery of the foot].
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Arteries↗
[Treatment of pseudarthrosis of the tibia by the method of traction].
The report is based on the experience with treatment, using the method of dosaged distraction of 39 pseudarthroses and unhealing fractures of the tibia in 38 patients. Twenty three patients showed osteomyelitis, 32-shortening of crural bones and 27-their deformation. With the aim of distraction the G. A. Ilizarov apparatus was employed. Up to data, the treatment is termined in all 38 patients. Healing was gained after 36 operations in 35 patients. No union was obtained after 3 operations of distraction the osteosynthesis in avascular forms of pseudarthrosis. Some regularities in formation of the regenerate and its reoconstruction during the process of lasting fixation were studied in 25 patients.
Adolescent↗
[Myelin basic protein. Structure, properties, function and role in diagnosing demyelinating diseases].
Physico-chemical properties and biological role of myelin basic protein (MBP)--one of the main myelin membrane proteins are reviewed. The data on MBP phosphorylation, methylation, fatty acid acylation and on interaction with lipid molecules in the human and animals bodies are presented. Much attention has been paid to the discussion of the diagnostic and clinical significance of the MBP, as the marker of demyelinating process in the central and peripheral nervous system.
Demyelinating Diseases↗
[Criteria of efficiency of transplantation of embryonic nervous tissue preparations in rats with 6-OHDA-impaired dopaminergic nigrostriatal system].
Effectiveness of transplantation of cells from embryonal nervous tissue of the ventral mesencephalon (VM ENT) and striatum (STR ENT) by apomorphin-induced motor asymmetry (APO-test), consolidation of the transplant (the degree of glyal reaction and amount of dopaminergic neurons) and blood serum levels of GFAP was studied for 3 months in Wistar rats with 6-OHDA-impaired dopaminergic nigrostriatal system. Marked therapeutic effectiveness was registered in VM ENT transplantation in the denervated striatum and in combined transplantation of VM ENT into the lateral cerebral ventricle simultaneously with STR ENT transplantation in the striatum. Separate transplantation of VM ENT in the lateral ventricle and STR ENT in the striatum had no positive effect on recovery of the dopaminergic nigrostriatal system. A correlation was found between the degree of glial reaction of ENT transplants, severity of rotation asymmetry and serum levels of gliofibrillary protein (GFAP). GFAP in the serum for lifetime assessment of transplant consolidation and prognosis of neurotransplantation efficiency was assayed.
Animals↗