[Lactate dehydrogenase X - specific enzyme of the testis].
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Animals↗
Biomedical subjects
E A Sevriukov
Publications and source records attributed to E A Sevriukov.
[Vitamin E content in cortical and medullary layers of mammalian kidney].
Vitamin E was quantified in the cortex and medulla of human, rat, canine and rabbit kidneys. For the all species investigated the vitamin concentrations, calculated both per g wet tissue weight and per mg tissue protein, was shown to be 1.4-2.2 times higher in medullary layer than in cortical one. The least vitamin E levels were observed in rabbit kidneys of which the cortical and medullary concentrations were 4.4 +/- 0.3 mkg (M +/- SEM) and 6.3 +/- 0.56 mkg per g wet tissue weight, respectively. In human renal cortex and medulla the vitamin concentrations were found to be 10.6 +/- 0.66 mkg and 16.2 +/- 1.1 mkg per g wet tissue weight, respectively. Close values were observed for other species studied as well. The increased renal medullary vitamin E concentrations are likely related to the higher phospholipid metabolism and enhanced prostaglandin synthesis in this tissue.
Animals↗
[Lipid peroxidation in the kidneys of rats with nephritis induced by nephrotoxic serum and proteinuria induced by albumin overload].
Lipid peroxidation (LPO) stimulated by ascorbate was studied in renal cortex of 20 rats with nephrotoxic nephritis (NTN) and of 9 rats with proteinuria induced by a 3-day course of i. p. injections of the human serum albumin. At the early stages of NTN (0.5 h. and 3 h.) LPO activities were of the same values as in control rats. A small decrease in renal cortex LPO was found on the 4-th day of NTN when nephrotic syndrome has been developed. A significant reduction in LPO activity was observed on the 16-th day of NTN characterized by a more pronounced nephrotic syndrome. LPO activity in renal cortex of the rats with albumin overload proteinuria was also reduced. An inhibitory effect of proteinuria on LPO activity in kidney is discussed.
Animals↗
[Zinc metabolism in male rats with a transplanted ovary].
Results of the research on the concentration of zink ions in the blood serum and prostate tissue, urine excretion level by means of heterotropic transplantation of the ovary to castrated rats (males) are presented. The degree of the organism estrogenization was assessed by determining the estradiol concentration in the blood serum by the radio-immune method. The research has shown that castration leads to a zink concentration decrease in the blood serum and its reduced excretion with the urine. A significant rise in the concentration of estradiol in the blood serum did not influence the zink metabolism in the organism.
Animals↗
[Proteolytic activity of human ejaculate].
Studies of the ejaculate proteolytic activity, viscosity, and protein concentration in patients with various spermatogenesis levels have revealed that viscosity and proteolysis directly depend on the count of spermatozoa in the ejaculate. Lower values were associated with azospermia. A clear-cut correlation was detected between these parameters and the inflammatory process in the prostate. Exacerbation of chronic prostatitis results in decrease of the ejaculate viscosity and activation of the proteolytic enzymes, this being confirmed by sperm protein electrophoresis, whereas the protein concentration is essentially reduced. These findings necessitate further investigations of the aforesaid parameters over the course of treatment for chronic prostatitis.
Adult↗
[Isolation of lactate dehydrogenase-C4 from human spermatozoa].
Lactate dehydrogenase-C4 (LDH) is a specific cell isoenzyme of spermatogenesis and spermatozoa of man and animals. Due to the lower levels of contaminating proteins, isolation of LDH C4 from spermatozoa had a lot of advantages. LDH C4 was isolated as follows: precipitation with ammonium sulfate, separation of LDH C4 from other LDH isoenzymes using immunosorbents, purification from contaminating proteins of oxamate-Sepharose. Antibodies to LDH1-3 immobilized on tresyl-Sepharose were used as immunosorbents. Elution of LDH C4 from oxamate-Sepharose by means of lithium lactate appeared inadequate therefore LDH C4 was eluted using 0.63 mM sodium pyruvate.
Chemical Precipitation↗