Kininase activity is present in saliva obtained from the oral cavity but not in saliva obtained by cannulation of the parotid or submandibular ducts. This activity originates from squamous epithelial cells from the oral mucous membranes. Experiments with various inhibitors indicated that the kininase activity of squamous epithelial cells differs from that of plasma, and resembles that of erythrocytes. Kinin-forming activity is present both in the glandular secretion and in the squamous epithelial cells.

Bradykinin↗

PubMed1964

PLASMA LEVELS OF VARIOUS BLOOD CLOTTING FACTORS IN GERMFREE RATS.

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Blood Coagulation↗

PubMed1963

Results of experimental intestinal strangulation obstruction in germfree rats.

Experimental low ileal strangulation obstruction has been produced in germfree and conventional rats. The mean survival time was 240 hours in the germfree rats and 44 hours in the conventional controls. 4 of the 10 germfree rats survived 15 or more days, whereas the 10 conventional animals were all dead within 2(1/2) days. The strangulation obstruction fluid from the germfree animals was sterile and non-toxic when injected into mice even after a fourfold concentration. The same fluid from the conventional animals contained a great number of microorganisms and caused death within 24 hours when injected intraperitoneally into mice.

Animals↗

PubMed1963

A STABLE SUBSTRATE FOR THE ASSAY OF PLASMA KININ-FORMING ENZYMES.

A substrate plasma for estimation of plasma kinin-forming enzyme activity in biological fluids has been prepared from human blood. Fresh citrated plasma was treated by a brief contact with a large amount of silicate powder, followed by heating to 56 degrees C for 1 hr, and finally by lowering its pH to 2 for 10 min. As an alternative to the final treatment with acid, disodium edetate was added to the plasma. The resulting final plasma did not show "spontaneous" plasma kinin activity on contact with glass, contained no kininase activity and did not inhibit plasma kinin enzyme activity of added specimens. The content of plasma kinin precursor in this final plasma was such that it could yield an amount of plasma kinin which corresponded to between 1 and 4 mug of synthetic bradykinin/ml.

Biological Assay↗

PubMed1957

Atresia vulvae.

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Female↗