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Biomedical subjects

E Adachi

Publications and source records attributed to E Adachi.

At least 127 records · Page 7Linked to original sources
PubMed1986

Pattern disappearance visually evoked cortical potential in the diseases of visual pathway.

Visually evoked cortical potentials in response to pattern-appearance, -disappearance, and -reversal stimuli were studied in normal subjects and in patients suffering from optic neuritis. It was found in normal subjects that pattern disappearance VECPs were as reliable as the pattern reversal ones. On the other hand, the appearance response showed interindividual differences which made its evaluation rather difficult. The same was true in the records of the patients. Both the mean peak latencies of the disappearance responses and those of the pattern reversal ones were prolonged significantly in optic neuritis, compared with those obtained from normal subjects. We concluded that the peak latencies of the disappearance response could be more confidently used than those of the appearance response for diagnosing optic neuritis.

Adult↗
PubMed1986

In vitro formation of hybrid fibrils of type V collagen and type I collagen. Limited growth of type I collagen into thick fibrils by type V collagen.

Type V collagen and type I collagen were obtained from human placentas by pepsin treatment, followed by salt fractionation. The precipitates formed at 37 degrees C from a mixed solution of type V collagen and type I collagen, reacted with antibodies to either type V collagen or type I collagen. The precipitates seen by electron microscopy were fine flexible fibrils, with a D-periodic banding pattern. The average diameter of hybrid fibrils was smaller than 50 nm, when the proportion of type V collagen exceeded that of type I collagen. Type V collagen directly interacts with type I collagen in forming hybrid fibrils, resulting in limitation of the growth of type I collagen fibrils into thicker fibrils. We propose that the fibrils with a predominant type V collagen content may occur in the pericellular environment of various tissues, as a basic structure in connecting basal laminae with interstitial collagen fibrils.

Chemical Precipitation↗
PubMed1986

Improved Kupffer's gold chloride method for demonstrating the stellate cells storing retinol (vitamin A) in the liver and extrahepatic organs of vertebrates.

This paper describes our modification of the classical gold chloride technique for the demonstration of the perisinusoidal stellate cells in the liver. The results of the method as introduced by von Kupffer (1876) are unpredictable. Using our modification, high quality gold preparations can be obtained. The method allows selective staining of retinol (vitamin A)-storing stellate cells in the liver and extrahepatic organs of various vertebrates. The sensitivity of the reaction is comparable to that of the fluorescence method for retinol. The technique is simple and the preparations keep for several years. Formol fixed specimens can be counterstained with Sudan III or hematoxylin. We have also developed a simple technique for making "sinusoid-net preparations," removing the parenchymal cells by supersonication. The clear visualization of the stellate cells that results has made it possible to study the distribution of these cells.

Animals↗
PubMed1985

In vitro formation of fine fibrils with a D-periodic banding pattern from type V collagen.

Type V collagen was isolated from human placenta by limited pepsin treatment and purified by salt fractionation. A solution of type V collagen was dialyzed at 4 degrees C against phosphate-buffered saline or against 0.02 M Na2HPO4. Aggregates formed under these in vitro conditions from a pure type V collagen solution were examined by electron microscopy. The aggregates were fine flexible fibrils. The fibrils formed during incubation at 25 degrees C were of relatively uniform diameter, 34.8 +/- 9.1 nm and did not show an axial banding pattern. When the specimen was incubated at 37 degrees C, the fibrils were of slightly larger diameter, 38.2 +/- 9.1 nm and almost all the fibrils had the axial repeat pattern of 67 nm. The ability of type V collagen to form banding fibrils is discussed in relation in the localization of the collagen in tissues.

Basement Membrane↗
PubMed1983

Fluctuation in the development of various skeletal muscles in the chick embryo, with special reference to AChE activity and the formation of neuromuscular junctions.

Acetylcholinesterase (AChE)-rich cytoplasmic granules in the developing myofibers increased remarkably until the establishment of neuromuscular junctions and thereafter decreased rapidly, whereas junctional AChE activities continued to increase (K. Wake, 1976, Cell Tissue Res. 173, 383-400). In the present paper, during the developmental course of the chick embryo, the temporal and regional gradients in differentiation of skeletal muscles at various sites were examined with special reference to the fluctuation of intracellular AChE activity. AChE-rich granules in each muscle throughout the whole body of chick embryos were observed. Since the distribution pattern of these granules changed regularly in the course of the muscle fiber development, advances of muscle differentiation in various sites of the body were compared. (1) The process of muscle development is more advanced in the trunk muscles than in the limb muscles. (2) The dorsal trunk muscles differentiate one day earlier than the ventral ones. (3) Within the same limb, proximal muscles differentiate approximately 24 hr ahead of distal ones. (4) The development of posterior limb muscles advances faster than that of anterior limb muscles. (5) Within the thigh muscles, the flexor muscles tend to differentiate earlier than the extensor muscles.

Acetylcholinesterase↗