[How things were at the Institute of Epidemiology and Microbiology 1941-1945].
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Academies and Institutes↗
Biomedical subjects
E Aldová
Publications and source records attributed to E Aldová.
New O and H antigens of the international antigenic scheme for Plesiomonas shigelloides.
A revised update of the International antigenic scheme for Plesiomonas shigelloides is presented. Twenty-six new O (O77-O102) and 10 new H (H42-H50 and H1a1d) antigens have been described since 1994. The sources of antigens are mostly human clinical strains, isolates from warmblooded animals and a few environmental cultures.
Animals↗
New serovars of Plesiomonas shigelloides--1992-1998.
Most of the 26 new O (O77-O102) and 10 new H (H42-H50) and H1a1d antigens were found in various P. shigelloides strains isolated from man and other mammals, birds, fish, and water and water insects, not only in the Czech Republic but also in 12 foreign countries.
Animals↗
Plesiomonas shigelloides in water and fish.
Plesiomonas shigelloides strains isolated from aquariums, surface water and fish were serotyped using the international antigenic scheme and a provisional scheme designed for environmental strains, called the "Schubert" scheme. A remarkable variety of serovars represented in both schemes was found, namely in surface water samples and in fish.
Animals↗
Characterization of Plesiomonas shigelloides from diarrheic children.
Infrastructure of 29 P. shigelloides strains isolated as the only positive finding from children with diarrhea (biochemical properties, antigenic structure, antigenic relationship to shigellae, ATB susceptibility and plasmids) were described. A big variety of 22 serovars in a relatively small number of strains was found, inclusive four new 0 (093, 094, 095 and 096) and one new H (H46) antigen. Some strains belonged to the so-called "Schubert antigenic scheme" the serovars of which come of surface water of small ponds in Germany.
Child↗
Detection and identification of Citrobacter sedlakii in the Czech Republic.
The biochemical characters of eight strains identified as Citrobacter sedlakii were investigated with the aid of six methods (tube tests, API 50 CH, API 20 E, MICROSCAN, BIOLOG, and CRYSTAL). All the strains were well defined on the basis of biochemical properties investigated with the aid of laboratory-prepared tube tests. The results obtained by the identification kits could not be correctly interpreted. Commercial diagnostic kits should include the reference data necessary for the identification of new Citrobacter species.
Bacteriological Techniques↗
New serovars of plesiomonas shigelloides--1996.
Eight new 0 (91-98) and H (46-49) antigens are described. Their reference strains come from Czech Republic, Cuba and USA. The majority of reference strains are of human origin. Some of the new antigens have been found in other strains coming mostly from water.
Antigens, Bacterial↗
[Biochemical indicators of Citrobacter sedlakii].
The authors describe eight strains identified biochemically as a new species of C. sedlakii in clinical material, surface water and the cutting surface of a melon. The majority of strains was isolated in the Czech and Slovak Republic.
Citrobacter↗
[The importance of serotyping Plesiomonas shigelloides].
The author presents an account of serovars of 432 strains of Plesiomonas shigelloides from various countries and sources; in addition to strains from man and animals also environmental strains. The most frequent serovars are O17 with three H antigens (H2, H11, H34) except for immobile strains, also O32:H4 and non-motile strains, O2:H1a1c and O44:H8. The other serovars did not reach the number of 10 strains, some serovars were found only one strain of each. Serovar O18:H2 was isolated in the course of 30 years only from cats and lynx and once from a pond in the FRG. As regards the distribution of different serovars, e.g. O68:H2 was found only in one district, while strains of the serogroup O17 come from various countries in the world. Between findings of the same serovar sometimes a 20-30-year interval elapses.
Animals↗
Serovars of Plesiomonas shigelloides.
Among 391 P. shigelloides strains, 330 were typable and found to belong to 43 O serovars. The most frequent O serovars were O17 (23%), O32 (7%), O12 (4.5%) and O5, O18 and O44 (3.8% each). The majority of the serovars represented, with almost 300 strains, were sporadically found in very distant countries of three continents (Europe, Asia, America), whereas only one serovar (O68) was isolated three times in one single district of South Bohemia (Ceské Budéjovice). It suggests that such an incidence of plesiomonads is only seemingly sporadic and that there must be many strains which are not detected owing to the imperfect cultivation methods used so far.
Animals↗
New serovars of Plesiomonas shigelloides 1992.
Fourteen new O (O77-O9O) and 4 new H (H42-H45) antigens were described. Seven O and 2 new H antigens were revealed among strains not agglutinating with antisera against 76 O and 41 H officially recognized serovars. The most frequent was serovar O80:H38 which was isolated in 5 countries (Sweden, Czechoslovakia, Bulgaria, Yugoslavia and Canada) from human material and from sewage and surface water.
Antigens, Bacterial↗
[Biochemical identification of aeromonads].
To assess the species of the genus Aeromonas in 178 strains isolated from human and animal materials and from the environment (water and hospital environment), examined along with 11 type strains of the best known species, a key was used the basis of which are in addition to biochemical tests defining the genus Aeromonas 12 tests: Voges-Proskauer, lysine decarboxylase, gas from glucose, haemolysis, gluconate oxidation, elastase, arabinose, mannose, saccharose, salicine, esculine hydrolysis, arbutine. Three tests--salicine, esculine hydrolysis, arbutine--differentiate A. hydrophila (2-3 positive) from A. sobria (0-1 positive).
Aeromonas↗
Aerogenic pasteurellas and Pasteurella-like organisms isolated in Czechoslovakia.
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Animals↗
Pilot studies on the occurrence of some infectious diseases in two different areas in south Yemen (Aden). Part I. Parasitology.
Two pilot studies on malaria, leishmaniasis, schistosomiasis and intestinal parasites were carried out in 104 children 6-15 years old from villages in the lowland and highland areas of South Yemen in November 1988. Some of the results presented are in the order lowland and highland. The occurrence of malaria parasites in blood smears was 6.7% and 3.8%. P. falciparum was detected only. The antimalarial IFAT antibodies were proved in 66.7% and 11.5% respectively. IFAT antibodies against leishmania were in 43.8% and 39.3% respectively of sera examined. Ova of S. haematobium diagnosed with the frequency 29.0% and 13.3% respectively. S. mansoni infection was found in 19.2% of examined children in highland only. From parasites found in stool specimens should be mentioned E. histolytica (42.3% and 36.8%), G. lamblia (34.6% and 35.1%). The other intestinal protozoa were less frequent. Further details of sex and age groups distribution are mentioned.
Adolescent↗
Pilot studies on the occurrence of some infectious diseases in two different areas in south Yemen (Aden) (part II. Microbiology).
This paper is the second part of the article dealing with intestinal bacteria. The findings are relatively poor in comparing with the frequency of intestinal protozoa. Among 83 stool specimens taken for bacteriological examination 14 isolations of different bacteria were proved. One S. muenchen was isolated from a 3 year old boy with fever, diarrhoea. Five cases of Alkalescens dispar 05 manit negative and 05 manit positive were identified. Two of these cases were without clinical symptoms. All were males aged 10-17 years. One isolation of E. coli EPEC 086 K6 H11 was in a 10 year-old boy with diarrhoea, four watery stools daily and cramps. Six cases of other E. coli were of different types, all with clinical symptoms. Of them three were males and three females at the age from 3-46 years. One case had a mixed infection of Citrobacter, E. coli and Klebsiella with diarrhoea, about 5 watery stools daily and abdominal pain. The frequency of intestinal bacteria in males was nearly three times higher than in females. The occurrence in age groups 10-20 was almost equal 20.0-22.2%, in 0-4 it was 42.9% and surprisingly low in 5-9 years old--3.4% only.
Adolescent↗
[Isolation of a rare serovar of Plesiomonas shigelloides].
The authors describe the isolation of a rare serovar Plesiomonas shigelloides from a patient with diarrhoea. The isolated strain belongs according to a combined Japanese-Czechoslovak antigenic scheme to serovar 024 H"o". The interesting finding draws attention to the possibility of routine departments to contribute to investigations of the incidence, importance and antigenic structure of this bacterial species.
Adult↗
Classification of strains of Pragia fontium, Budvicia aquatica and of Leminorella by whole-cell protein pattern.
SDS PAGE protein patterns of 37 H2S-positive strains of species belonging to the family Enterobacteriaceae including the genera Budvicia (11 strains) and Leminorella (L. grimontii--3 strains, L. richardii--4 strains) were compared with 10 strains of species Pragia fontium. All strains under study form well separated clusters with overall similarity C = .49. Clusters are separated in the range of C = .68-.83. They display high homogeneity, only one strain of Edwardsiella tarda clusters with budviciae. Strains of Pragia form two distinct clusters separated from other genera. Electrophoretograms of two strains which do not group as expected are analyzed and results discussed. Results support evidence that strains designated Pragia fontium deserve independent treatment as a new species.
Bacterial Proteins↗
[The Aeromonas genus].
Aeromonas hydrophila manifested itself since its discovery in 1891 as a pathogen of cold-blooded and warm-blooded animals and man. Aeromonads cause intestinal and non-intestinal disease. The genus Aeromonas comprises: A. hydrophila, A. sorbria, A. caviae, A. veronii, A. schubertii, i.e. mesophilie species and as to psychrophilie immobile species. A. salmonicida and A. media. For warm-blooded animals and man mesophilie motile species are important as pathogens. A. veronii is an ornithine-decarboxylase positive species, A. schubertii is mannitol-negative, A. caviae is non-haemolytic and VP-negative. It is difficult to differentiate. A. hydrophila with aerogenic and anaerogenic strains from A. sobria. Several practical differential diagnostic tests were suggested by Janda et al. and Joseph et al.: hydrolysis of esculine, KCN, arabinose and salicin are usually positive in A. hydrophila, in A. sobria usually negative. Existing species of mesophilie aeromonads, however, do not correspond to some strains which are found. Therefore Arduino et al. divided their aeromonads into DNA-hybridization groups: for A. hydrophila there were 5, for A. caviae 2 and for A. sobria 1 hybridization group. Biochemisal characteristics corresponded to the hybridization groups. For isolation of aeromonads from faeces selective media with ampicillin must be used and possibly enrichment in alkaline peptone water. Evidence of pathogenity factors is similar as in E. coli,: detection of adhesins and enterotoxin or cytotoxin by means of tests commonly used in cholera and E. coli. The types of adhesins are differentiated by means of fucose- galactose- and mannose resistant haemagglutination.(ABSTRACT TRUNCATED AT 250 WORDS)
Aeromonas↗