PubMed Health⌕ Search

Biomedical subjects

E Annapurna

Publications and source records attributed to E Annapurna.

5 recordsLinked to original sources
PubMed1980

Enterotoxicity of group F vibrios.

Recently a group of organisms resembling Aeromonas hydrophila has been isolated from human diarrheal cases from marine and estuarine environment. These organisms are tentatively designated as group "F" vibrios. Live cells of 23 strains isolated from different sources were tested for their enterotoxicity in the adult rabbit ileal loop model. All but two caused fluid accumulation comparable to that obtained with Vibrio cholerae 569B. An inoculum of 10(4) viable bacteria could give rise to gut loop reaction. Culture filtrates of the 19 strains tested including one giving negative reaction with live cells also caused accumulation of fluid in loops.

Animals↗
PubMed1977

Enterotoxicity of Aeromonas hydrophila.

Live cells and cell-free culture supernates of 50 strains of Aeromonas hydrophila isolated from diarrhoeic and healthy human faeces, drinking water, sewage, the river Ganges and faeces from domestic animals caused accumulation of fluid in ligated ileal loops of adult rabbits. The amount of fluid produced was comparable to that of a toxigenic strain of Vibrio cholerae. Three of the strains gave positive reactions only after two passages in ileal loops of rabbits. Inocula of about 10(3) viable cells and 0-25 ml of culture supernate caused fluid accumulation in the loops. The enterotoxic factor was inactivated at 60 degrees C for 20 min. and 65 degrees C for 10 min., was precipitated with ammonium sulphate and was non-dialysable; these results indicate the protein nature of the enterotoxin. An inoculum of 40 microgram of crude toxin caused as much fluid accumulation as larger inocula. The only histopathological change in the loops was depletion of mucus from the goblet cells.

Aeromonas↗