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Biomedical subjects

E Aoki

Publications and source records attributed to E Aoki.

At least 91 records · Page 5Linked to original sources
PubMed1980

2'-3'-cyclic nucleotide 3'-phosphohydrolase activity in the central nervous system of a myelin deficient mutant (Shiverer).

Shiverer is a recessive autosomal mutant characterized by the deficiency of the myelin in the central nervous system. 2',3'-Cyclic nucleotide 3'-phosphohydrolase (CNPase) activity of the various parts of the central nervous system from the Shiverer did not differ significantly from those of the control. The analysis of the protein profiles of the purified myelin from the Shiverer showed a greatly decreased proportion of proteolipid protein, and almost complete absence of small and large basic proteins, and intermediate protein. Woldgram protein accounted for a much larger percentage of the total myelin protein than is the case in myelin from the control. Proteolipid protein, small and large basic proteins, and intermediate protein were found to be undetectable or decreased in various parts of the central nervous system from the Shiverer. Morphological observation by optic and electron microscope showed that the myelination of the optic nerve was equally affected as the spinal cord.

2',3'-Cyclic-Nucleotide Phosphodiesterases↗
PubMed1979

Biochemical and immunohistochemical studies on dysmyelination of quaking mutant mice in vivo and in vitro.

Dysmyelination in the central nervous system of the quaking mutant mouse was studied biochemically and immunohistochemically. We found, by measuring CNPase activity, that myelination in the central nervous system of quaking mice was affected to a different degree in different areas. The pallium cerebri was the most severely affected and the medulla and spinal cord were least affected. The density of astroglia observed by GFA staining wash higher in the white matter of quaking mice than in controls, but the total area of the white matter in the cerebellum was smaller in the quaking mice than in the controls. The DNA content in the pallium cerebri and brain stem showed no increase and that of the cerebellum was even lower in quaking mice than in the controls. Hypertrophy of the astroglia was observed in the white matter of the cerebellum of quaking mice, though Bergmann astroglial fibers in the molecular layer did not show any hypertrophy. The cerebella of quaking mice in primary culture showed very poor myelination under the phase-contrast microscope. However, Purkinje cells from the quaking mutants appeared normal with regard to Bodian silver impregnation, hematoxylineosin staining, and Purkinje cell specific P 400 protein. Addition of the conditioned culture medium of qk/qk to the culture of the control cerebellum did not interfere with the myelination. We concluded that the cause of dysmyelination in the quaking mouse could be a genetic defect of the oligodendroglia rather than hypertrophy of the astroglial cells.

2',3'-Cyclic-Nucleotide Phosphodiesterases↗
PubMed1979

A resected case of pulmonary multiple fibro-leiomyomatous hamartoma--light and electron microscopic study.

A light and electron microscopic study of a case of multiple pulmonary fibro-leiomyomatous hamartoma is described. Like the previous reports, the patient was an old woman with no complaint. The histologic features were characterized by mingling with rich fibroblasts and mesenchymal cells. The definite diagnosis was made by the features which were detected ultrastructurally. In this case, we considered that the fibrous component was more predominant than the leiomyomatous component.

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