Biomedical subjects
Publications and source records attributed to E Azoulay.
Candida tropicalis strain 101 possesses a hydroxylase system when grown on tetradecane as the carbon source which is active towards hydrocarbons and fatty acids. This system including cytochrome P(450) and NADPH-cytochrome c reductase has been localized exclusively in the microsomal fraction.
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Three double mutants were prepared from single mutant chl-r strains of Escherichia coli K12 lacking nitrate reductase activity. When extracts of these three double mutants were mixed, nitrate reductase activity was reconstituted.
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A method for the preparation of protoplasts from Candida tropicalis cultivated on n-tetradecane is described. This essentially consists of replacing the mannitol-sorbitol solution of the classical helicase technique by 1 m magnesium sulfate and lowering the pH to 4.1 during incubation in the presence of helicase. The protoplasts thus prepared behave like intact cells and are capable of consuming oxygen in the presence of n-tetradecane, n-decane, 1-decanol, and glucose.
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