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Biomedical subjects

E B Mechetner

Publications and source records attributed to E B Mechetner.

42 records · Page 3Linked to original sources
PubMed1979

[Synthesis of globin mRNA in cultured murine erythroleukemic cells, transformed by Rauscher leukemia virus].

In contrast to cultured erythroleukemic cells transformed by Friend leukemia virus, in the cultured erythroleukemic murine cells transformed by Rauscher leukemia virus, the synthesis of haemoglobin cannot be induced after treatment with 1--2% dimethylsulfoxide, actinomycin D and proteolytic enzymes. Study of disturbances in the haemoglobin synthesis in this cell culture revealed that globin 9S-mRNA is either absent or present in negligible amounts in the culture. The data obtained suggest that the disturbances in haemoglobin synthesis occur at the level of transcription or processing of globin mRNA, rather than at the level of translation.

Animals↗
PubMedDate not supplied

[Selective cytotoxic effect of an immunotoxin on human erythroid tumor cells].

The possibility of efficient directed elimination of human erythroblastoid cells by the conjugate of IgM-monoclonal antibody HAE9 directed against the erythroblast antigen and the A-chain of a plant toxin ricin has been demonstrated. The conjugate contained 2 molecules of A-chain per one antibody molecule. The efficiencies of the cytotoxic effect of native ricin and the conjugate were compared according to the number of binding sites on the surface of K562 cells as well as to the internalization rate of these molecules. As was shown, that the number of binding sites for the antibody approaches 2.7.10(4) molecules/cell, K a being equal to 1.7.10(8) M-1 while for ricin these indices constitute 2.4.10(5) and 4.6.10(8) M-1. Almost 100% of antibodies and 36% of ricin are internalized within 10 min at 37 degrees C. At a concentration 10(-11) of native ricin and 10(-10) of immunotoxin the 50% inhibition of growth of K562 cells carrying the erythroblast antigen on their surface is observed.

Antibodies, Monoclonal↗
PubMedDate not supplied

[Erythroblast antigen expression in murine hematopoietic and nonhematopoietic cells. 2. An immunofluorescent study in pre- and postnatal ontogeny using monoclonal antibodies].

The expression of erythroblast antigen (Ag-Eb) in cell membranes during pre- and postnatal mouse development was studied by immunofluorescence using the monoclonal antibody MAE-15. Ag-Eb was detected in embryonic liver, spleen, epithelia of intestine, various glands and skin, as well as in extraembryonic tissues (yolk sac and trophoblast). In pregnant mice positive immunofluorescence was observed in placenta and on the surface of decidual cells in uterus. In adult non-pregnant mice Ag-Eb expression was detected not only in membranes of erythroid cells, but also in non-hemopoietic tissues, such as epithelia of various glands, intestine, kidney and testis, brain endothelium, basal layer of epidermis, and intercalated discs of the heart muscle. A possible role of Ag-Eb in processes of cell transport is discussed.

Animals↗
PubMedDate not supplied

[Expression of the erythroblast antigen on hematopoietic and nonhematopoietic mouse cells. 1. The flow cytofluorimetric analysis of stem, committed and differentiated bone marrow cells].

Expression of the erythroblast antigen (AG-EB) on the membrane of the mouse hemopoietic cells was studied using a FACS II flow cytofluorimeter with monoclonal antibodies MAE15. It was shown that AG-EB was present on the surface of 14% of the late committed erythroid precursors (CFU-3) and of practically all more mature erythroid cells. The role of AG-EB in maturation of the erythroid cells is discussed.

Animals↗