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E B Vishnevskaia

Publications and source records attributed to E B Vishnevskaia.

15 recordsLinked to original sources
PubMed2006

[The specific features of bacterial isolation and drug resistance of Mycobacteria in extrapulmonary tuberculosis].

The reduced viability and enzymatic activity of the pathogen from the foci of extrapulmonary tuberculosis are twice more frequently than those in pulmonary tuberculosis. The isolation of mycobacterial L-forms and the use of polymerase chain reaction significantly enhance the efficiency of the etiological diagnosis of extrapulmonary tuberculosis. There are extremely poor trends for drug resistance to develop in mycobacteria, in 2005 the rate of which was 92.5% in pulmonary tuberculosis and 83.4% in extrapulmonary tuberculosis. However, multidrug resistance is much less common in extrapulmonary tuberculosis than in pulmonary tuberculosis; the rates were 33.9 and 73.9%, respectively. Levofloxacin resistance was also completely preserved so the reserves for the treatment of extrapulmonary tuberculosis are higher than those in pulmonary tuberculosis.

Antitubercular Agents↗
PubMed2003

[Drug resistance of Mycobacterium tuberculosis in the North-West of Russia].

The morbidity of primary and secondary drug resistance (DR) of Mycobacterium tuberculosis (MBT) was studied in the North-West of Russia during 1991-2001. The frequency rate and structure of, mainly, secondary DR MBT was investigated in tuberculosis of different localizations on the basis of data provided by clinics of Saint-Petersburg Research Institute for Phthisiopulmonology (PRIP) during 1990-2000. A sharp increase of primary DR MBT (in the North-West of Russia) up to 35-45.5% was detected in the Murmansk, Arkhangelsk and Saint-Petersburg Regions as well as in the Republic of Karelia, an increase of up to 61% was registered in the Kaliningrad Region. The frequency rate of secondary DR MBT went up by 1.5-2 times reaching 80-86% in the Murmansk, Arkhangelsk and Kaliningrad Regions. According to the PRIP clinics, there is also an essential growth of DR MBT in all tuberculosis localizations with a trend of the stability structure shifting towards poly-resistance, which accounts for 65.2% in pulmonary tuberculosis and for 33.6% in extra-pulmonary tuberculosis.

Antitubercular Agents↗
PubMed2002

[Polymerase chain reaction and ligase chain reaction in the diagnosis of gynecological tuberculosis].

The polymerase chain reaction (PCR) and the ligase chain reaction (LCR) were evaluated and compared on 55 gynecological samples collected from women with active gynecological tuberculosis (Group 1), gynecological diseases other than tuberculosis (Group 2), and active tuberculosis elsewhere in the body without evidence for gynecological tuberculosis (Group 3). Acid fast staining and culture of Mycobacteria appeared to be ineffective in all specimens. The sensitivity of both amplification methods was 94.7% (one of the 19 patients with non-tuberculosis). The LCx method showed a very high inhibition by specimens (64.1% of negative samples versus 16.7% for PCR and LCx could be useful tools in the diagnosis of gynecological tuberculosis.

Adult↗
PubMed2002

[The detection rate and clinical and diagnostic values of L forms of the pathogen in patients with pulmonary or extrapulmonary tuberculosis].

The detection rate and clinical and diagnostic values of L-forms of pathogens were determined in patients with pulmonary and extrapulmonary tuberculosis. Simultaneous culturing the specimens for typical and L forms of Mycobacterium tuberculosis (MBT) increased the number of positive results by isolating only L-forms by 10.3% in patients with pulmonary tuberculosis and by 27.7% in those with extrapulmonary tuberculosis. No bacterial isolation in tests only for typical forms of MBT is shown not to be true and a purposeful search for L-forms of MBT enhances the efficiency of a bacteriological test. This is of great significance in confirming the specific nature of the disease, its progression, in choosing a treatment policy, in evaluating is efficiency, in defining prognosis, and in correcting preventive measures in the focus of tuberculous infection. With extrapulmonary tuberculosis, the tuberculous nature of isolated L-forms has been evidenced by the polymerase chain reaction.

Female↗
PubMed2001

[Identification of L-forms of Mycobacterium tuberculosis complex by polymerase chain reaction (PCR)].

Polymerase chain reaction (PCR) was used to verify the tuberculous origin of L-forms isolated from clinical non-respiratory samples from patients with extrapulmonary tuberculosis. PCR was made by using cultured L-forms obtained from negative and positive cultures. PCR used a total of 60 cultured L-forms different in the morphology of colonies and the rate of growth. The total count of L-forms yielding positive amplification with M. tuberculosis complex-specific primers was 51 (85%). L-form passages were subjected to PCR analysis. A total of 14 third-generation L-forms were examined. They turned out to be positive. Thus, the fact that L-forms isolated from nonrespiratory clinical samples from patients with tuberculosis are most commonly L-forms of M. tuberculosis was genetically substantiated.

Bacteriological Techniques↗
PubMed2000

[The problems of the PCR analysis of low-bacterial-count tissue samples in extrapulmonary tuberculosis].

PCR was used to study clinical materials from patients with extrapulmonary tuberculosis or diseases of nontuberculous etiology (renal tissue, scrapes of the endometrium and uterine cavity, bony fragments). It was shown that when the oligobacillary material analyzed, a concurrent study of some fragments of the sample largely enhanced the efficiency of PCR detection of Mycobacterium tuberculosis DNA. For the biopsy and surgery tissue specimens, PCR provided the best results in isolating DNA for the alkaline lysis test.

Bone and Bones↗
PubMed1998

[Plasmid profile of mycobacteria spread on the territory of the Russian Federation].

The plasmid profile of different species of mycobacteria vegetating in the Russian Federation was first studied. All the drug-resistant mycobacterial strains of tuberculosis were found to be truly polyresistant. There was a certain association between the plasmid carriage and the high viability of the mycobacterial population in nontuberculous mycobacteria.

Anti-Bacterial Agents↗
PubMed1998

[Biological features and problems in detection of extrapulmonary tuberculosis pathogen].

The viability, enzymatic activity and Mycobacterium tuberculosis drug resistance was compared in pulmonary and extrapulmonary tuberculosis. To decrease oxygen in the culture medium baseline values and to use various oxidants as protectors were shown to be promising in enhancing the efficiency of cultural diagnosis of extrapulmonary tuberculosis.

Antitubercular Agents↗
PubMed1998

[Ways to decrease inhibition of the polymerase chain reaction by the components of the clinical specimens].

Inhibition of the polymerase chain reaction (PCR) by components of the analyzed specimen is an important problem in analysis of clinical material by this method. The inhibitory activity of clinical material can be decreased by additional purification of DNA by gel filtration on microcolumns. The method was developed using blood-containing specimens and tried on many clinical specimens. Commercial PCR kit for detecting Mycobacterium tuberculosis was used. The proposed method for DNA purification notably improves the efficacy of detecting the agents by PCR.

Artifacts↗
PubMed1998

[Specific features of DNA isolation for polymerase chain reaction in extrapulmonary tuberculosis].

While testing samples by the polymerase chain reaction (PCR), a procedure for DNA isolation is advisable adapted for different types of materials; thorough DNA purification is of the greatest importance in testing blood-containing samples, deep sample lysis is of particular importance for osseous tissue samples. A differential approach to isolating DNA from clinical samples allows the efficiency of PCR to be enhanced.

DNA, Bacterial↗
PubMed1994

[Isolation and analysis of lipid antigen from M. tuberculosis cell walls].

A method has been developed for isolation of an antigen based on nonpolar lipids from M. tuberculosis cell walls, its specific and nonspecific fractions separated, and composition determined by thin-layer chromatography. Ninety-two blood sera of tuberculous patients and 50 ones from healthy donors were examined in enzyme immunoassay with this antigen. Use of this antigen in serologic tests helped detect in patients' sera antibodies to mycobacterial lipids.

Antigens, Bacterial↗
PubMed1991

[Determination of tuberculosis antibodies using an immunoenzyme assay in lyophilized samples of capillary blood].

The aim of this work was to find the technique of enzyme immunoassay (EIA) of capillary blood lyophilized on a filter paper disk. The author has selected a buffer on the basis of egg yolk aqueous extract, that permits blocking of nonspecific binding in EIA but not preventing the specific antigen-antibody interactions. The results of analyses of capillary blood lyophilized drops and of blood serum samples, carried out in 7 tuberculous patients and 6 donors, coincided. The results were tested in 57 blood samples.

Antibodies, Bacterial↗
PubMedDate not supplied

[The combined use of different antigens in immunoenzyme analysis for tuberculosis antibodies].

The technique of enzyme immunoassay with a lipid antigen and with two antigens in a single well has been developed. Blood sera of 75 patients with tuberculosis and 50 donors were analyzed. Use of a combined antigen was found to increase by 16% the sensitivity (antituberculosis antibody detection rate) of the enzyme immunoassay test system. Use of the suggested method will improve the diagnostic significance of the serologic test without increasing the number of analyses.

Antibodies, Bacterial↗