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Biomedical subjects

E Baldoni

Publications and source records attributed to E Baldoni.

At least 37 records · Page 2Linked to original sources

Fluctuations in arylsulphatase activity in the rabbit endometrium during the sexual cycle.

Histochemical and biochemical studies were performed to verify the presence of arylsulphatase A (ASA) and B (ASB) in the rabbit uterus. Fluctuations in the activity of these sulphatases during the sexual cycle were also studied. Some structural and functional properties of purified ASA were determined. The results indicate that arylsulphatases are active in the endometrium during both the estrogenic and progesteronic phases. The activity of ASA was much more intense than that of ASB; it increased during estrus and decreased during the post-ovulatory phase. ASB activity, however, decreased during estrus and increased during the post-ovulatory phase. The significance of these fluctuations is discussed in relation to the action of sexual hormones and physiological substrates of arylsulphatases.

Anestrus↗

Arylsulphatases in the rabbit oviduct: postovulatory changes tested by histochemical and biochemical procedures.

A histochemical and biochemical study of the activity of arylsulphatases A and B was carried out on the oviduct of female rabbits during the first days after mating. The histochemical results demonstrated that the ampullary and the isthmic epithelial cells have a positive reaction to the sulphatases during the whole of the postovulatory period tested. The enzymatic activity is mainly localized in the basal cellular cytoplasm. The biochemical results confirmed that both arylsulphatase A and B are active. Arylsulphatase A activity is more intense in the ampulla than in the isthmus and it increases during the whole of the postovulatory period; in the isthmus the activity increases up to 72 h, thereafter decreasing again. The arylsulphatase B activity is always lower than arylsulphatase A activity; maximum activity is reached between 66 to 72 h after mating. The arylsulphatase B is relatively higher in the ampulla than in the isthmus. The biological role of these enzymes is discussed in relation to the regulation of the sulphated glycoconjugates.

Animals↗

Arylsulphatases A and B in the oviduct of female rabbits in anestrus and estrus conditions.

An investigation has been carried out on the activity of arylsulphatases A and B (ASA and ASB) in the oviduct of rabbits in anestrus and estrus conditions. The histochemical and biochemical results obtained have demonstrated that both arylsulphatases are present in the ampulla tract and in the isthmic one, and that the estrus determines a considerable increase in the specific activity of ASB. Hypothesis are advanced on the probable-meaning of the variations in the activity of these enzymes, particularly in relation to the secretion of glycoconjugates by the epithelium of the oviduct.

Anestrus↗

[Why aid to the elderly should not remain an affirmation of principle but should become an active reality].

The most frequent causes of the feeling of "being a burden" in elderly people dependent on others due to chronic disabling pathologies are described. Often this feeling is the main, and sometimes the only reason for their isolation from the community. Suggestions are made for the removal of this feeling, which is felt to be caused mainly by social attitudes and the style and structure of existing social services. It is felt that education in social attitudes should be directed not only to the invalid concerned but also, and primarily, to the community as a whole. In terms of treatment, rehabilitation and social facilities, various appropriate activities are suggested. A careful reorganisation of the institutions offering these services is also recommended.

Aged↗

Ultrastructural histochemical study on glycoconjugates of the submandibular gland of rabbits.

An ultrastructural histochemical study was carried out on submandibular glands of rabbits. Stainings were performed with dialysed iron (DI), high iron diamine (HID), tannic acid uranyl acetate (TA-U), tannic acid-ferric chloride (TA-F) sequence, and periodate-thiocarbohydrazide-silver proteinate (PA-TCH-SP) method. It was demonstrated that neutral glycoproteins are present in the cells with dark granules of the preterminal tracts, and that neutral and acid glycoproteins are present in the cells with light granules of the terminal tracts. Result are discussed and compared to other previously obtained histochemical and biochemical data.

Animals↗

Fine structure of the rabbit submandibular gland during the embryonic development.

The development of the submandibular gland has been examined in rabbit embryos from the 22nd day to the birth. The 2 main types of secretory cells, which synthetize dense serous granules or large pale mucous granules can be identified from the 24th day of development. A third type of cells, characterized by granules having different densities, has been identified in the preterminal tracts. The possibility that these cells are similar to some small cell with an highly dilated endoplasmic reticulum found in adult rabbit submandibular glands is discussed.

Animals↗

The ultrastructure of the rabbit submandibular gland.

The secretory granules of the terminal and pre-terminal tracts of the rabbit submandibular gland display distinct features, while the cell morphology is quite similar. Among these cells, some smaller ones presenting an highly dilated endoplasmic reticulum have been identified in the pre-terminal tracts. Some hypothesis have been formulated concerning the function of these cells.

Animals↗