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Biomedical subjects

E Benjamini

Publications and source records attributed to E Benjamini.

At least 73 records · Page 4Linked to original sources
PubMed1975

Immune plasma-dependent cytotoxicity of immune and non-immune peripheral lymphoid cells for target cells coated with bacterial outer unit membrane.

The development of a model system for use in the study of lymphoid cell cytotoxicity to bacterial membrane antigens was attempted. In this system 51 Cr-labelled chicken red blood target cells were coated with pieces of the outer unit membrane of leptospirae rather than with soluble antigens. Using the model system to study dog peripheral immune lymphoid cell cytotoxicity to coated target cells we found that both immune and non-immune lymphocytes are antibody dependent for the expression of their cytotoxicity. It was also found that the unit membrane preparation from leptospirae can serve as a good antigenic stimulant to immune dog lymphoid cells as measured by increased [3H]thymidine uptake.

Animals↗
PubMed1975

Antibodies to fentanyl.

Immunization of rabbits with carboxyfentanyl-bovine gamma-globulin conjugate induced antibodies of high titers capable of binding with fentanyl. The antibodies exhibited high average association constants (approximately 1 times 10-7 liter/mol) and were highly specific to fentanyl since no cross-reactivity was observed for a variety of test compounds including other opiates and analgesics. Serological cross-reactivity was observed only for chemicals representing various portions of the fentanyl molecule. These high-affinity, highly selective antibodies have been successfully employed in a radioimmunoassay for fentanyl. Serum concentrations of fentanyl, after administration of 100 and 21 mug/kg/ i.v. in dogs, were determined by this radioimmunoassay. The specificity of these antibodies as compared with the specificity of the pharmacological (opiate) receptor is also discussed.

Animals↗
PubMed1970

Studies on the immune response to a characterized antigenic determinant of the tobacco mosaic virus protein.

THE FOLLOWING PEPTIDES HAVE PREVIOUSLY BEEN SHOWN TO BIND SPECIFICALLY WITH ANTIBODIES TO TMVP: (a) An eicosapeptide representing residues 93-112 of TMVP and having the sequence Ileu-Ileu-Glu-Val-Glu-AspNH(2)-GluNH(2)-Ala-AspNH(2)-Pro-Thr-Thr-Ala-Glu-Thr-Leu-Asp-Ala-Thr-Arg. (b) Its C-terminal decapeptide. (c) Its C-terminal pentapeptide. (d) N-octanoyl-C-terminal-tripeptide. (e) (Lys)(4)-C-terminal-pentapeptide. (f) (Lys)(7) C-terminal-pentapeptide. The present communication deals with the investigation of several parameters of the immunological activity of the peptides. The results show that none of the peptides tested were immunogenic in guinea pigs, nor did they stimulate the incorporation of (14)C-thymidine by spleen cells derived from TMVP-primed animals. Results also showed that all of the peptides tested could elicit specific delayed and immediate skin reactions in TMVP-sensitized guinea pigs, and furthermore, that the peptides could specifically inhibit the migration of peritoneal exudate cells derived from these animals. The elicitation of delayed skin reactions and the ability to inhibit migration of peritoneal exudate cells were independent of carrier specificity.

Animals↗