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Biomedical subjects

E Beregi

Publications and source records attributed to E Beregi.

At least 19 recordsLinked to original sources
PubMed1991

Effect of aging on cold tolerance and thyroid activity in CBA/Ca inbred mice.

Relationships between cold tolerance, serum levels of thyroxine (T4), thyronine (T3), and thyrotropine (TSH), and thyroid morphometry have been investigated in male CBA/Ca inbred mice at various ages through their life span. From the data obtained it appeared that there was an age-related decrease in cold tolerance up to 18 months of age which was followed by an increase, the age effect being most apparent in relation to cold resistance and cold tolerance during the recovery period following cold exposure. The age-related changes in cold tolerance appeared to be associated with changes in the serum concentrations of T3, T4, and TSH. In contrast to the T3 serum levels which showed a decrease at 36 months, the thyroxine contents showed a perceptible decrease from the age of 12-18 months onwards. A similar pattern was observed for the TSH levels, with a peak at 21 months, followed by a decline at 30 months. A relationship with age between serum T4 level and thyroid weight was indicated together with structural changes in the thyroid gland, particularly during senescence, for example the size and number of thyroid epithelial cells had become enlarged by 30 months of age.

Acclimatization↗
PubMed1991

Comparative study of the morphological changes in lymphocytes of elderly individuals and centenarians.

The number of white blood cells, the morphological changes of the peripheral lymphocytes and some immunological variables were examined in 118 centenarians and in 499 healthy subjects of 60-89 years of age. There was no change in the absolute number of leucocytes, lymphocytes, or T or B cells with age. The occurrence of a myelin-like structure in the mitochondria and the presence of lipofuscin in the cytoplasm of lymphocytes increased with age and showed a correlation with the absolute number of T cells. A correlation could be observed between the absolute number of B cells, serum IgG level and the presence of antinuclear factor.

Aged↗
PubMed1991

Effect of cigarette smoke and 2-mercaptoethanol administration on age-related alterations and immunological parameters.

In old CBA/Ca mice the effect of cigarette smoke was compared with that of 2-mercaptoethanol (2-ME) treatment. It could be stated that spontaneous death was more frequent in animals kept in cigarette smoke than in the control animals. Prevalence of hepatocellular carcinoma was higher in animals kept in cigarette smoke than in the controls. After 2-ME treatment the occurrence of hepatocellular carcinoma was significantly lower and animals without disorders were more frequent than in smokers. Body weights were lower in animals kept in cigarette smoke and differences in organ indices could be observed, too. Immunological changes were also demonstrated: in mice kept in cigarette smoke the reactivity against a foreign antigen such as sheep erythrocytes (SRBC) was lower, while after 2-ME treatment it was higher than in their controls using direct plaque formation technique. The ratio of normal reactivity (against SRBC) and autoreactivity (against mouse erythrocytes) showed a decrease in smoker animals, and an increase in the 2-ME-treated ones. The experiments showed a deleterious effect of cigarette smoke and a beneficial effect of 2-ME on age-related alterations.

Aging↗
PubMed1989

Health and living conditions of centenarians in Hungary.

Demographic data of 218 centenarians in Hungary were collected, and 123 were examined by a medical team. This data showed that the centenarians are from families with a history of longevity. A larger proportion of the centenarians live in the capital, Budapest, than in the rural areas. The centenarians are mostly manual workers (rural) and nonmanual workers. Most of them live with their families. The centenarians are healthier than the general population, drug consumption is low, and they have good adaptative abilities. We compared the clinical laboratory findings of the centenarians with that of 60-80 year olds. Though age-related changes were present in the centenarians, these age-related biological changes did not hinder them from reaching very old age. Finally, we found a few differences in the immunological, clinical laboratory, and morphological profiles of this population. We anticipate more extensive elaboration on this data in future publications.

Aged↗
PubMed1989

Decreased cytosolic free calcium concentration of aged human lymphocytes in resting state.

Cytosolic free calcium concentration was measured in lymphocytes from individuals over the age of 80, using quin2 and fura-2 calcium indicators. The average intracellular free calcium concentration of the samples was 62 nM, which value is roughly half the adult (age between 35 and 55) level (116 nM). It is supposed that the decline in immune function of aged individuals is connected to the decrease in free calcium concentration in their lymphocytes. We also discuss the consequences and the adaptive character of this decrease.

Adult↗
PubMed1988

Effect of 2-mercaptoethanol on some metabolic indices of ageing of CBA/Ca inbred mice.

It has been shown in several studies that 2-mercaptoethanol (2-ME) improves the life span and aspects of life performance of laboratory animals. To obtain further details on the beneficial effects of 2-ME, a long-term study has been performed on male CBA/Ca inbred mice treated with this antioxidant. Four month-old mice were each given 4 micrograms of 2-ME in physiological saline via intraperitoneal (i.p.) injection 3 x per week. Measurements were made of the following: cold tolerance (heat performance), apparent total body protein turnover (T1/2), changes in the major lipid and fatty acid compositions of the liver, superoxide dismutase activity and formation of malondialdehyde and observations on a range of pathological changes. It was found that the basal rectal temperatures of the treated mice were higher and in the oldest group, heat performance capacity was better than those of the controls. After about 1 year of age the apparent biological half-life time of total body protein (T1/2) was observed to be shorter in the treated mice. Significant increases were observed to occur in the proportions of the polyunsaturated fatty acids in the lipids of the liver in the mice injected with 2-ME. Although no differences were observed in the superoxide dismutase activities, malondialdehyde concentrations in the livers of the experimental mice were significantly increased. Autopsy data showed that Dunn-sarcomas associated with amyloidoses occurred more frequently in the untreated mice.

Aging↗
PubMed1987

Biological changes and diseases in aged CBA/Ca mice.

Cold adaptation, apparent protein metabolism, life span, body and organ weights, organ indices, mitochondrial changes in the lymphocytes, and frequency of diseases were examined in 661 (untreated) inbred CBA/Ca male mice. The rectal temperature proved to be lower in aged mice, i.e. the rectal temperature of old animals immediately after cold exposure was more distinctly decreased than that of young ones. The apparent protein metabolism measured by 75Se-selenomethionine turnover showed that biological half-life (T 1/2) values in the aged are almost linearly elevated. In very old animals the values are decreased. Body weight decreased significantly and continuously, spleen and liver indices decreased and heart index increased with age in healthy animals. The number of diseased animals increased with age peaking at the age of 751-900 days. After 900 days the number of diseased animals decreased. The most frequent diseases in the old animals are: hepatocellular carcinoma, amyloidosis and pulmonary adenocarcinoma. The mitochondria of spleen lymphocytes showed degenerative changes not associated with diseases, thus they can be considered as age-related biological changes.

Aging↗
PubMed1987

Comparative morphological study of age related mitochondrial changes of the lymphocytes and skeletal muscle cells.

The age dependent electron microscopic changes of the mitochondria in a mitotic cell (lymphocyte) were compared with those of a postmitotic cell (skeletal muscle cell) in humans and in CBA/Ca mice. Two types of age related changes could be observed in the mitochondria of lymphocytes and skeletal muscle cells: (i) degenerative changes; (ii) giant mitochondria. The frequency of the mitochondrial changes increased with age and were more frequent in the skeletal muscle cells than in the lymphocytes.

Adult↗
PubMed1985

Autoimmunity and normal immune functions in aged humans.

The high frequency of ANA, A-LDL and RF in advanced age suggests that AABs are present in the majority of aged subjects. CIC incidence determined by three methods is far below AAB incidence; only the Clq solubility test suggests an increased CIC incidence in aged as compared to young subjects. Simultaneous occurrence of AABs of different specificities or CIC determined by two or three methods is rare and both AAB and CIC levels are usually low. AAB prevalence in CIC-positive individuals seems to depend on the specificity of the AAB. CIC positivity is associated with relatively low Clq concentrations; however, usually not with Clq concentrations below the normal range. Neither ANA nor CIC positivity seems to correlate with DNA synthetic response to PHA, but ANA positivity may be associated with low responses to allogeneic cells. ANA positivity and, to a lesser extent, CIC positivity seems to be connected with enhanced killer cell activity. The concept of some AABs and CIC as autoregulatory factors of the humoral immune system compensating for the thymus-dependent regulation in old age is stressed.

Aged↗
PubMed1984

Longitudinal study of lymphocytes.

Longitudinal investigations of the peripheral lymphocytes of 91 healthy individuals were made over a period of six years. It was found that in aged subjects degenerative changes occurred in the mitochondria of the T cells. The number of subjects with damaged mitochondria in the lymphocytes increased significantly between 60 and 80 years of age. Six years after the first investigation, the number of individuals with damaged mitochondria also increased, with a peak between 70 and 79 years.

Aged↗
PubMed1983

Lipofuscin in lymphocytes and plasma cells in aging.

The age-related changes in spleen and lymph node lymphocytes and plasma cells of Wistar rats and in the peripheral lymphocytes of healthy human individuals were examined. Similar changes were described in human and rat lymphocytes and plasma cells consisting of mitochondrial lesions. The cristae of the mitochondria disappeared and were replaced by myelin-like structure, lamellar structure, electron-dense and electron-translucent material. It is supposed that this material corresponds to lipofuscin.

Adult↗
PubMed1983

Long-term observations on the effect of polyadenylic acid in mice of different ages.

In order to better life performance, polyadenylic acid (poly (A) ) was given intraperitoneally to CBA/Ca mice for almost a two-year period. This substance, as one of the components of double-stranded polynucleotides (like poly A:U), is known to improve some immune responses of the aging organism. Five approaches (changes in body-weight, adaptation to cold stress, biological half-life of body proteins, mortality and pathology) were applied to test the effects of this substance on life performance. It was found that the beneficial effects of double-stranded polynucleotides cannot be mimicked by polyadenylic acid only, despite its anti-senescence effect, namely, it accelerates the apparent protein turnover, cf., biological half-life. Polyadenylic acid shortens life-expectancy (because of the higher mortality rate of mice). Possible mechanisms of these actions are discussed.

Aging↗
PubMed1983

Relationship of mitochondrial damage in human lymphocytes and age.

Authors examined the peripheral lymphocytes of 366 healthy individuals electron microscopically. They described age related degenerative changes in the mitochondria. The lamellar structure, electron-dense and electron-opaque material shown in the mitochondria of the lymphocyte proved to be similar to lipofuscin. The relationship of mitochondrial damage in lymphocytes and age was analysed, too. They stated that the number of patients with mitochondrial changes in the lymphocytes increased from 50 years of age until 80 years, but after 80 years decreased.

Adult↗
PubMed1982

Lymphocyte subpopulation changes by aging.

Peripheral blood lymphocyte suspensions of healthy young and aged subjects were tested for the percentage of (1) E-rosetting cells by three different modifications of the rosette technique; (2) alpha-naphthylacetate esterase positive cells of different staining patterns; (3) IgG-Fc receptor positive cells; (4) C3 receptor positive cells; (5) labile and stable bound surface immunoglobulin positive cells; and (6) cells bearing different classes of immunoglobulins on their surface or intracytoplasmically. Age dependent changes were registered both within the T-cell and the B-cell subpopulations, some of which may be due to in vivo activation of lymphocytes. Attention is called to some technical aspects of lymphocyte subpopulation determinations and to the significance of quantitative changes in the proportions of lymphocyte subpopulations in respect to the age dependent functional changes of lymphocytes.

Adult↗
PubMed1980

The influence of influenza vaccinations on human peripheral blood lymphocytes relating to aging.

Twenty healthy, aged more than 60 years were compared to 20 healthy young volunteers less than 40 years following influenza vaccinations. 0.5 ml, 10 U. of killed influenza vaccine Hong Kong B/873 was repeatedly given intramuscularly. Blood was collected before the vaccination, 6 days, respectively 6 weeks after vaccinations. The purified venous blood lymphocytes were examined by immunohistological and sheep erythrocyte rosette-forming methods. The absolute number of T and B lymphocyes was counted. It has been found that increase of IgG-bearing cells was higher in young persons, than in the elderly both in the first and in the revaccination. The absolute number of IgM-bearing lymphocytes increased in the aged after influenza vaccinations. The vaccine had no significant effect on the absolute number of IgA-bearing lymphocytes. The absolute number of T lymphocytes participating in the immune reaction showed a transitory increase solely in young persons. Experiments aiming to demonstrate the reduced activation of B lymphocytes in the elderly with the influenza vaccinations. It can be concluded, that the proliferation ability of the immunoglobulin-bearing B cells and T cells is reduced due to old age.

Adult↗