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Biomedical subjects

E Bey

Publications and source records attributed to E Bey.

At least 19 recordsLinked to original sources
PubMed2006

Use of topical negative pressure with a lipidocolloid dressing: results of a clinical evaluation.

OBJECTIVE: To evaluate the protection and acceptability of Urgotul wound dressing in the local management of acute or chronic wounds receiving topical negative pressure (TNP) therapy. METHOD: This was a prospective multicentre non-comparative open-label trial. At each dressing change the investigating physician clinically evaluated and photographed the wound. Planimetric measurement was undertaken and wound depth was assessed at the start and end of the treatment. Follow-up was undertaken until deemed clinically unnecessary by the investigator. RESULTS: Sixty-six patients were included (42 acute wounds and 24 chronic wounds) and followed up for an average of 17 days. Dressing changes were deemed entirely painless in 52% of cases (compared with 18% at baseline) and pain between two consecutive dressing changes was absent in 66% of cases (34% at baseline). Removal of the TNP-interface dressing combination was considered'very easy' or 'easy' in 94% of cases and adherence to the wound was recorded as 'absent' in 88%. On average, the dressings were changed every 3.8 +/- 1.1 days (all wounds were considered), and wound area and depth were reduced by 19% and 54% respectively by the end of the follow-up period. CONCLUSION: Use of the interface dressing in combination with TNP substantially reduced the pain caused by dressing changes. It therefore makes more acceptable the use of this technique, which aims to optimise the management of wounds that are sometimes considered to be in a therapeutic impasse.

Acute Disease↗
PubMed2005

[Microsurgery: History of instrumental vascular anastomoses, our experience with eversion-stapling using VCS forceps].

One century, after Carrel in 1906, technics of vascular surgery are the same. After two world wars, peace surgery has been improved by war surgery. Microscopy surgery gave a new way for vascular surgery which became microsurgery with specific instrumentation. We have move from the developing period of microsurgery in the 1970s, to the fully matured period of microsurgery in the 1980s and the the development of clinical free flaps. The 1990s must be the turning point from autogenous tissue transplantation to allogenic transplantation. Ethic comity keeps keys of future! About microvascular anastomoses, many instrumental technics are explored but no-one is better than the classic manual suture. For us, the best instrumental technic is the anastomose with titanium clips VCS((R)) but we only use it in good situation without difficulties.

Adult↗
PubMed2001

[Vertical alveolar distraction osteogenesis of the posterior edentulous mandible: a case report].

When it is necessary to increase the vertical height of the residual alveolar ridge, alveolar distraction osteogenesis has numerous advantages compared to other preprosthetic surgical procedures. It is frequently used for this purpose in the anterior region because of the obvious accessibility. The authors present a clinical case of edentulous posterior mandible, with insufficient vertical alveolar bone height, treated by alveolar distraction osteogenesis leading to three titanium fixtures. They explain their choice and discuss the preliminary results.

Alveolar Process↗
PubMed2001

[Vascularized bone grafts in the treatment of hemifacial microsomia].

A systematic and theoretic review is performed on the vascularised bone donor sites that may be useful in the case of hemifacial microsomia. A review of the literature reveals that the iliac crest and the scapula are the most commonly used vascularised bone grafts. A case study is included in the paper, showing an hemifacial microsomia reconstruction using a composite fibular vascularised bone graft. They highlight the criterias they think fundamental for choosing the vascularised bone graft donor site regarding the aims of the reconstruction of a Pruzansky grade II or III.

Adult↗
PubMed2001

[Postoperative monitoring of free fibular grafts by dynamic magnetic resonance imaging. Preliminary results in three cases of mandibular reconstruction].

The vascularized free fibular graft has been used in mandibular reconstructive surgery since 1975. This technique has been progressively developed, and it is now the procedure of choice for mandibular reconstruction although in certain postoperative circumstances it can be difficult if not impossible to monitor bone vitality. However, bone vascularization can be detected by dynamic magnetic resonance imaging (MRI), as this technique has been experimentally and clinically validated in the early diagnosis of osteonecrosis. The aim of this study was to evaluate the efficacy of MRI for the postoperative monitoring of vascularized free fibular grafts in human mandibular reconstruction. Dynamic contrast-enhanced MRI was used to study the variation in contrast over time following injection of gadolinium contrast medium, and to evaluate the degree of bone marrow perfusion of the fibular graft. This variation in signal intensity was visualized in the form of a curve, i.e., a perfusion curve for the bone marrow region. An examination was performed in three patients at different postoperative times and under different conditions. In one case, MRI confirmed the presence of fibula blood supply in spite of the necrosis of the adjacent fascio-adipose layer. In this article, the methodological difficulties have been discussed, particularly as regards data processing, and the present results have been compared with the findings in the literature. Dynamic MRI is a simple, reliable, non-invasive technique and its use in the postoperative monitoring of bone marrow perfusion and vascularized free fibular grafts permits a determination of the status of the latter following surgery, i.e., whether there is an adequate blood supply or not.

Aged↗
PubMed2000

[Bone vascularization. Anatomic and physiological considerations, surgical deductions concerning osteosynthesis procedures and vascularized bone graft].

The authors report some general considerations concerning the bony anatomy and the bone blood flow. They describe the vascularization of the long bones involving arteries, veins, capillaries and lymphatic vessels. The double systemic and local control of the bone blood flow is evaluated. The local factors are numerous: autoregulation, nervous regulation as well as humoral, metabolic and cellular factors. Some surgical deductions are then discussed concerning osteosynthesis procedures and biomechanical properties of vascularized bone grafts.

Bone Transplantation↗
PubMed2000

[Facial artery. Embryological review, descriptive and functional anatomy based on a review of the literature].

After a summary of the embryology and anatomy of the arterial blood supply of the face, the authors present the descriptive anatomy of the facial artery and its eight most frequent branches. Variations of the origin, course and division of the artery are then described. The literature contains 3 to 5 different descriptions of the course of the artery. The authors considered 4 of these descriptions to be relevant: I (angular), II (nasal), III (superior labial), IV (hypoplastic). Seventeen different branches have been described, many of which are inconstant. The superficial and deep anastomotic network is described, with emphasis on the subdermal network and that of the transverse facial artery. The various vascular regions of the face, first described by Mitz and Whetzel, are described. Based on these observations, and on Lasjaunias'theory of the triple hemodynamic balance, the authors propose a functional analysis of the arterial blood supply of the face. According to this analysis, the anatomic variations observed are determined by regional hemodynamic balances. These observations influence the design and creation of certain local facial flaps, such as the submental, nasolabial and buccinator flap. They may also influence present concepts concerning the hemodynamics of arteriovenous malformations of the face.

Arteries↗
PubMed1999

[Mesenteric cystic lymphangioma].

BACKGROUND: Cystic lymphangioma is an uncommon congenital malformation usually encountered in children and often discovered fortuitously. CASE REPORT: A 46-year-old man was hospitalized for acute alcoholic hepatitis with edematoascitic decompensation. The abdominal and pelvic CT scan showed homogeneous hepatomegaly and a tumoral process involving the terminal ileal loops without signs of occlusion or node enlargement. Pathology reported benign mesenteric multicystic lymphangioma. DISCUSSION: Cystic lymphangiomas account for 7% of all intra-abdominal cystic formations. The ileal localization predominates with little or no clinical expression. Ultrasonography and CT scan provide complementary information on the relations with other organs. Magnetic resonance imaging is currently the gold standard allowing precise diagnosis of the anatomic relations and identifying intracystic hemorrhage. The pathology examination is required to eliminate possible benign multicystic mesothelioma which may occasionally take on an aggressive form. Complete surgical excision provides cure.

Humans↗
PubMed1997

[Microsurgical reconstructions of the lower face, after projectile injuries. Value of fibular flaps. Apropos of 20 cases].

Projectile trauma of the lower face causes major functional damage and disfigurement. Reconstruction with tissue flaps, after microsurgical revascularization to guarantee tissue vitality, can restore satisfactory mental function. We report 26 mental reconstructions, including 20 using free fibular flaps which, due to its vascular pedicle and length of the available bone, appears to be the best adapted for reconstruction of the lower face. When used with cutaneo-aponevrotic flaps, only one operation is needed.

Adult↗
PubMed1997

[Dentigerous cysts. Developmental aspects. Apropos of 3 cases].

Dentigerous cysts, also called follicularor corono-dental cysts, are malformative odontogenic cysts in the WHO classification of odontogenic tumors. We review the general characteristics of these frequent odontogenic tumors which always occur in associated with an included tooth. Diagnosis is based on clinical features, X-ray findings and pathology results. We report the evolutive aspect in three patients of different ages.

Adolescent↗
PubMed1997

[Mechanical vascular clips with an automatic stapler in reconstructive microsurgery. Apropos of 16 clinical cases].

From October 1995 through March 1997, we performed 30 microsurgical reconstructions and achieved microvascular anastomoses in 16 with an automatic stapler (VCS) which allows insertion of titanium clips in the everted vessel walls. The mean age of the patients (15 men, 1 woman) was 42 years. Mean vessel diameter of the donor and recipient sites was 2 mm for the arteries and 3 mm for the veins. Recipient vessels had suffered radiation damage in 3 cases. Micro-anastomoses were performed in 26 cases, with end-to-end sutures in 24 and end-to side sutures in 2. There were 11 arterial procedures including 3 bypasses and 15 venous procedures with 1 bypass. Peroperative thrombosis occurred in 2 cases and were treated by undoing the anastomoses and resuturing manually. There were no early or late postoperative vascular complications. This clinical experience confirms the advantages of this mechanical approach to microanastomotic procedures for small vessels as previously demonstrated in experimental work: a system avoiding transfixation, rapid procedure, reliability.

Adult↗
PubMed1994

Detection by DNA fingerprinting of somatic changes during the establishment of a new prostate cell line.

The establishment of a new prostate cell line (BM1604) from a human prostatic adenocarcinoma is reported. The line was rapidly established by culture of tissue on an extracellular matrix, previously laid down by culture of non-related cells. The method has been shown to work well, and other prostate lines have recently been cultured in this way. The cells have a doubling time of 28 h. DNA fingerprinting comparison of the genome from the tumour, the germline and the cells shows that somatic mutations have occurred in the tumour and that clonal selection has clearly occurred in establishment of the line. Many somatic mutations are apparent in the selected cells, which are now stable in culture. This method and the cells may be a useful addition to the limited material available for the in vitro study of prostate cells.

Adenocarcinoma↗
PubMed1985

The PLC/PRF/5 human hepatoma cell line. I. Reevaluation of the karyotype.

The karyotype of the PLC/PRF/5 (Alexander) human hepatoma cell line was identified at passage +/- 110, prior to attempting in situ hybridization studies to determine the chromosomal localization of the hepatitis B virus (HBV) DNA integration sites. The karyotype was established by means of G-, Q-, and sequential C-banding techniques. Multiple consistent numerical and structural abnormalities were detected. These were compared with the original published unbanded karyotype of this cell line (at passage less than 25) and also with a previously published banded karyotype (at passages 60-90). Despite minor differences in the compared banded karyotypes (which are probably interpretational), the concordance in modal number and morphological karyotypic similarities over the course of 10 years indicate that this cell line is stable in vitro.

Carcinoma, Hepatocellular↗
PubMed1985

The PLC/PRF/5 human hepatoma cell line. II. Chromosomal assignment of hepatitis B virus integration sites.

The chromosomal sites at which hepatitis B virus (HBV) DNA is integrated into the genome of the hepatocellular carcinoma (HCC) cell line, PLC/PRF/5 were investigated in an attempt to understand the mechanisms by which hepatitis B virus may induce malignant transformation. In situ hybridization of an HBV DNA probe to metaphase chromosomes of the PLC/PRF/5 cell line, followed by statistical analysis, identified three integration sites; these were 15q22-q23, 11q22, and 18q12. In particular, hybridization to chromosome #15, which is present in four copies in complete metaphases of this cell line, was highly significant (p much less than 0.0005).

Adult↗
PubMed1984

A comparison of three methods for titration of poliovirus vaccines.

Two methods for in vitro endpoint titration of poliovirus--the roller tube and the microtitration assay--were compared with each other and with the plaque assay, using secondary vervet monkey kidney cells and Vero cells as indicators. The roller tube method is the most reliable under difficult working conditions, but is otherwise cumbersome and expensive. The microtitre method is the most economical and the plaque assay the most sensitive. By suspending freshly trypsinized indicator cells with the virus dilutions before planting, it was possible to simplify the microtitre method considerably. The sensitivity of the plaque assay was improved for Vero cells by absorbing the virus onto freshly planted monolayers. The method was scaled down to a semi-micro level by using 24-well cell culture trays. The slower rate of plaque development under a low calcium overlay medium facilitated a more accurate plaque count.

Animals↗
PubMed1980

A human liposarcoma cell line.

The properties of a new cell line derived from a human retroperitoneal liposarcoma are described. The cells do not grow at low cell concentrations and contain in their cytoplasm large numbers of droplets that stain with Oil Red O. No indication of the presence of endogenous virus particles could be found. The mean chromosome number per cell is 36, with several constant markers, the most conspicuous of which is a large submetacentric marker due to a translocation between chromosomes 4 and 11, which is present in every cell. The liposarcoma cells show an enhanced uptake of [14C]acetate compared to normal human fibroblasts.

Acetates↗
PubMed1979

Chromosome studies on a human liposarcoma cell line.

Numerical and structural chromosome analysis of a human retroperitoneal liposarcoma cell line maintained under standard cell culture conditions revealed a very stable hypodiploid mode. If the cells were not trypsinized for several generations, a near-triploid stemline, which was generally a duplication of the hypodiploid mode, emerged. Some chromosomes appeared to be relatively stable pairs (1, 2, 7, 9, and 12), but most had "lost" one homolog or both (4 and 21) or were rearranged into "new" marker chromosomes. Quantitation of the genetic material showed a loss of 12.0 +/- 3.7% per spread. Only one characteristically long marker chromosome, which is present in every cell, could be identified with certainty as a translocation between chromosomes 4 and 11. Several of the marker chromosomes showed interstitial negatively staining regions with the trypsin-Giemsa method.

Cell Line↗