Biomedical subjects
Publications and source records attributed to E Bláhová.
The authors demonstrate on the case of a 21-year-old female diabetic (type I), who had been well compensated with insulin for five years, the life threatening procedure of a popular healer. By eliminating insulin he induced severe keto acidotic coma.
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A simple prediction test for a combination of hyperthermia and chemotherapy checked on three experimental tumors and cells of the normal murine thymus is described. The testing procedure is based on checking the incorporation of labeled 125I 5-iodo-2-deoxyuridine into the DNA of tested cells affected solely by the enhanced temperature or by the cytostatic itself, or by a combination of both agents. The statistical analysis of repeated results demonstrated the reproducibility of the test. Testing of the potentiation effect of different non-cytostatic substances with enhanced temperatures also gave results corresponding to findings of other authors, performed in vivo or by the use of other techniques. Thus, this simple method is recommended for a screening, before considering the individual use of the combination of hyperthermia and chemotherapy in patients.
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On the basis of an experimental model of the Lewis lung carcinoma a possibility was checked of obtaining basic kinetic data about the individual proliferation of tumour cells by a method of measuring the incorporation of labelles 125I 5-iodo-2-deoxyuridine intratumorously with a subsequent measurement of the retained activity in the tumour. These activity, which resides in the tumour for a time longer than 24 hours depends on the fraction of cells, that in the synthetic part of the cell cycle at the time of the injection.
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