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Biomedical subjects

E Bosman

Publications and source records attributed to E Bosman.

8 recordsLinked to original sources
PubMed2006

[Transvaginal aspiration as first treatment of ovarian follicular cysts in dairy cattle under field circumstances].

On dairy farms covered by the ambulatory service of the Department of Farm Animal Health, 55 Holstein-Friesian cows with suspected ovarian follicular cysts on the basis of rectal palpation underwent confirmatory transrectal ultrasonography. An ovarian follicular cyst was confirmed in 28 cows (50.9%) and was treated by single transvaginal-guided needle aspiration. Cows with a corpus luteum or a luteinized cyst were not treated. After aspiration, 82.1% (n=23) of the cows showed oestrus behaviour at 13.3 +/- 6.0 days. Artificial insemination was performed during the first heat after cyst aspiration and resulted in a pregnancy rate of 64.2%. In conclusion, single transvaginal-guided needle aspiration of ovarian follicular cysts is an easy and good method for the treatment of follicular cysts. Moreover, it is a safe and good alternative method for the manual, active rupturing of cysts during rectal palpation.

Animals↗
PubMed1994

A comparative study on the diagnostic sensitivity of rodent sperm and embryos in the detection of endotoxin in Earle's balanced salt solution.

PURPOSE: The bioassay used by most IVF units to assess culture media is the mouse embryo test. The limitations of this assay are well known. The objective of this study was therefore to evaluate the in vitro bioassay potential of rodent sperm, in terms of relative sensitivities to endotoxins, and to compare the results with the routine mouse embryo and human sperm tests. RESULTS: The greater sensitivity of rodent sperm than of mouse embryos was evident in this study. A further advantage in using the mouse sperm test was the time (4-6 hr) in which endotoxins could be detected. CONCLUSION: This rapid sperm test proved to be inexpensive, convenient, and invaluable for detecting potential sources of cytotoxicity.

Animals↗
PubMed1993

Pregnancy outcome after in vitro fertilisation at the Pretoria unit.

This retrospective study was conducted to ascertain the success rate of a university-based in vitro fertilisation (IVF) programme. Over a 4-year period a total of 881 patients was aspirated with an 81.8% embryo transfer (ET) rate. This resulted in 150 biochemical pregnancies (20.8% per ET), and 92 births that produced 100 babies were recorded (12.8% per ET). Multiple pregnancies accounted for 8.7% of births and 6.5% of the 100 babies presented with minor congenital abnormalities. Delivery took place by caesarean section in 46% of cases. Birth mass of babies ranged from 1.06 to 4 kg with a mean of 2.944 +/- 0.629 kg. Mean gestational age was 260 +/- 18.2 days. Twenty-three percent of babies born were preterm, 13.8% of these were twins and presented with a low birth mass. It was concluded that the conception rate of patients did not differ with regard to the number of IVF attempts but that those who conceived during the first two attempts had a significantly better chance (P < 0.05) of carrying to term.

Abortion, Spontaneous↗
PubMed1990

In vitro fertilisation when normal sperm morphology is less than fifteen per cent. Results of in vitro fertilisation and embryo transfer at H.F. Verwoerd Hospital, Pretoria.

The outcome of in vitro fertilisation and embryo transfer in 90 couples where the husband's normal sperm morphology was less than 15% were analysed. Based on the percentage of morphologically normal spermatozoa the patients were divided into three groups: group A--normal morphological features 0-5%; group B--6-10%; and group C--11-14%. A control group had normal morphological features greater than or equal to 15%. The fertilisation rate and number of embryos transferred was not significantly different in these groups. However, the pregnancy rate per embryo transfer was significantly different for groups A and B compared with group C and the control group (0% and 5.9% for groups A and B v. 13.9% and 18.3% for group C and the control group, respectively). It is concluded that when the normal sperm morphology is less than 11% the prospect of a pregnancy is poor.

Embryo Transfer↗
PubMed1990

Successful pregnancy despite retrograde ejaculation. A case report.

In retrograde ejaculation sperm is passed into the bladder causing infertility. Among patients seen at the Reproductive Biology Research Unit of the University of Pretoria retrograde ejaculation is relatively uncommon (0.004%), but is a potentially treatable cause of infertility. The technique for collecting sperm, sperm processing and recovery described was successful and resulted in a pregnancy after transfer of 1 four-cell embryo.

Ejaculation↗
PubMedDate not supplied

Sperm adenosine triphosphate concentrations before and after freezing as a marker of cryolysis.

Frozen semen samples are generally used today for artificial insemination and in vitro fertilization (IVF) and have various advantages above freshly produced semen. A disadvantage is that substances may leak out of the sperm if the membrane is damaged during freezing. In this study sperm ATP concentration was determined before freezing and after thawing and correlated with fertilization in an IVF program. After freezing and thawing the average sperm motility decreased significantly (p < or = .0001). Sperm ATP concentration was also significantly lower after freezing and thawing (p = .0004). Sperm ATP concentration after thawing did not correlated with fertilization. ATP concentration was a good marker of cryolysis but not of fertilization.

Adenosine Triphosphate↗