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Biomedical subjects

E Bourret

Publications and source records attributed to E Bourret.

8 recordsLinked to original sources

Rheological behaviour of saturated polyglycolysed glycerides.

Seven saturated polyglycolysed glycerides (Gelucires) of melting points varying from 42 to 53 degrees C and hydrophilic-lipophilic balance values from 2 to 14 were selected. Their rheological behaviour was determined by adjustment of the flow curves to the Ostwald power-law and by statistical assessment of the flow index. The flow of Gelucires was slightly shear thickening. This shear thickening rose when the temperature and the lipophilic specificity of the Gelucire increased. This behaviour accounted for a reorganization of the particles under the shear which became easier when the temperature increased and when the degree of condensation of the polyethylene glycol chains decreased with lipophilicity of the Gelucires.

Delayed-Action Preparations

[Determination of micro-quantities of serum lithium by atomic absorption spectrophotometry. Significance and applications].

The dilution of serum and the ionic components having an usual effect upon the lithium assay is examined about their influences in the determination of lithium microquantities. These are discussed according to the technique selected as well as the normal ionic variations that may be come across in serums. The method was applied to determination of serum lithium rate in a group of old subjects. The got lithium distribution is unsymmetrical at lower rates and no influence of age or sex was obvious.

Aged

[A simple hemolytic plate method for complement activity estimation (author's transl)].

The previous single radial haemolysis method is simplified for complement activity assay in human serums. The proceeding a "one point" test is described. This new technique is remarkable by its simplicity and rapidity. A study of the precision shows tha possibility of to make use of this simple "one point" hemolytic plate method.

Complement System Proteins

[A study of the single radial haemolysis optimization for complement haemolytic activity assay (author's transl)].

The experimental conditions for single radial haemolysis are analysed in order to optimize the haemolytic plate method for the assay of human total complement. The amount of haemolytic antibody used for sensitization of red cells, the concentration of sensitized sheep erythrocytes, the thickness of agarose layer, and the diameters of wells all exercise an influence on the area of haemolysis. The effects of these parameters are investigated with a view to obtaining the clearest reading and greatest sensitivity of the haemolytic activity plate method, and at the same time verifying the validity of the assay and the precision of the human serum haemolytic titres. This work reports the optimal experimental requirements with regard to the factors studied for minimizing the sources of error in complement haemolytic plate assay.

Complement System Proteins