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E Bryl

Publications and source records attributed to E Bryl.

22 records · Page 2Linked to original sources
PubMedDate not supplied

Level of NK cytotoxic activity in the elderly aged more than 80 years.

A group of healthy volunteers aged 60-93 years, was evaluated with respect to their NK activity. These people have been under careful medical observation for 3 years and were selected according to Senieur Protocol criteria. NK activity of each person was checked at least three times at regular 12-month intervals with the chromium release test and twice at the same interval of time with a single cell cytotoxicity assay. NK activity of 60-79-year-old females was in a similar range of values as that of young females (20-35 years) and was lower than that of the males of the corresponding age groups. The females aged 80-88 years revealed the highest NK activity. The chronically ill 80-88-year-old females, on the other hand, were characterized by a marked decrease of NK activity in relation to their age counterparts. NK activity of both the females and males significantly declined at the age of 89-93 years. The significance of NK activity for survival is discussed.

Journal Article↗
PubMedDate not supplied

Elderly high NK responders are characterized by intensive proliferative response to PHA and Con A and optimal health status.

In this paper we verify a hypothesis emerging from recent immunogerontological studies assuming that an elevated level of NK activity in the elderly may be regarded as a factor compensating for a decreased reactivity of T cells. According to this assumption a high NK activity should be associated with a poor reactivity of T cells. In our study the mitogenic responses of lymphocytes of the elderly and the young high and low NK responders to phytohemagglutinin (PHA) and Concanavalin A (Con A) were measured. A high level of NK activity was not found, as would be expected from the hypothesis, to be associated with a poor reactivity to PHA and Con A. In contrast, the mitogenic response to PHA and Con A was intensive in both the young and old high NK responders while weak proliferative responses to T cell mitogens were characteristic of the young and old low NK responders. Thus, our results do not support the assumption of an association between a high NK activity and a low proliferative response to T cell mitogens in the elderly. We also found that both the elderly and the young high NK responders were characterized by an optimal health status.

Journal Article↗
PubMedDate not supplied

Heparin and nitric oxide treatment in experimental acute pancreatitis in rats.

The aim of this study was to investigate the impact of L-arginine (nitric oxide synthase substrate), L-NG-nitro-L-arginine (nitric oxide synthase inhibitor), and heparin on the pancreas microcirculation, serum IL-6 level and microscopic alterations of the pancreas in acute pancreatitis in rats. Acute pancreatitis was induced by 4 i.p. injections of cerulein (15mg/kg). Microcirculatory values were measured by means of laser Doppler flowmetry 5 h after the first cerulein injection. Remarkable histopathological changes in the pancreas, including parenchymal necrosis, an elevation of serum IL-6 level, and a significant drop of pancreatic capillary perfusion was observed in rats with nitric oxide synthase inhibition. L-arginine improved the pancreatic microcirculation but worsened the microscopic alterations within the pancreas. Heparin had a beneficial effect on the microcirculatory values, serum IL-6 concentration, and morphologic changes. Authors conclude that inhibition of nitric oxide synthase aggravates acute pancreatitis. L-arginine treatment improves pancreatic perfusion but potentiates morphological alterations. Heparin, improving the microcirculation and inflammatory changes within the pancreatic gland, may be considered as a promising therapeutic agent in acute pancreatitis.

Acute Disease↗