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E Budde

Publications and source records attributed to E Budde.

At least 37 records · Page 2Linked to original sources

[Phage typing of rough E. coli strains isolated from urine (author's transl)].

A differentiation of E. coli rough strains is generally not well established in bacteriological urine diagnosis although these strains are relatively often isolated from urine samples of patients with urinary tract infections (see table 2). An exact characterization of rough strains seems to be necessary especially for the distinction between recurrent and reinfection during therapy and follow-up studies. The application of phage typing for characterization of E. coli rough strains isolated from urine is reported in the following paper, using a set of 13 phages (A-M) introduced by Marsik and Parisi (8). Out of 284 E. coli rough strains 166 (58.4 per cent) were found to be typable. Altogether 56 different phage patterns were observed. The most common phage patterns are presented in table 1. It is evident that GHK is the most frequent pattern occurring in 7 per cent of the strains investigated. The next most common patterns are M and BCDEIJLM (4.9 and 3.5 per cent, respectively). These, together with AM (3.2 per cent) and F (3.2 per cent) account for 21.8 per cent of all the rough strains tested. From the results it is concluded that phage typing may be a valuable tool for differentiation of E. coli rough strains.

Adult↗

[Demonstration of antibody-coated bacteria in the urine of pregnant women].

Urinary sediments of 64 pregnant women with significant bacteriuria which has been demonstrated several times before, have been investigated for the presence of antibody coated bacteria. Antibody titers were determined in the corresponding serum samples in addition and the results were compared with the clinical diagnosis. In 50% of pregnant women with pyelonephritis antibody coated bacteria could be demonstrated, while 60% of them had elevated serum titers. Taking into account both results in 80% of the pregnant women the clinical diagnosis of pyelonephritis could be confirmed. Pregnant women with asymptomatically significant bacteriuria never gave positive results, whereas pregnant women with cystitis mainly did have negative results. The demonstration of antibody coated bacteria in the urinary sediments of pregnant women with significant bacteriuria as well as the detection of elevated serum antibody titers with the indirect immunofluorescence technique against the urinary strain were found to be of importance for the valuation of a significant bacteriuria.

Antibodies, Bacterial↗

[Urinary and serum o-antibodies in patients with chronic pyelonephritis (author's transl)].

Urine (24-hour-specimen, 100-fold concentrated) and serum samples from adults with chronic pyelonephritis have been investigated for O antibody against the strain isolated from the urine by means of the indirect hemagglutination and indirect immunofluorescence technique. Using the hemagglutination technique about one third of the urine showed the presence of O antibody. The highest titer demonstrated was 1:16. O antibody determined by the immunofluorescence technique could be demonstrated in 54.9% of the urine samples. Titers up to 1:256 were found. Serum O antibody corresponded to those found in adults with chronic pyelonephritis. In general there was no correlation between urine and serum O antibody. The diagnostic and prognostic value of the results is discussed.

Adult↗

[Microbiological diagnosis of pyelonephritis].

Microbiological methods occupy an important place in the diagnosis of pyelonephritis. Taking into consideration still open problems, the principles of the bacteriological and serological diagnosis of infections of the urinary tract are discussed.

Bacterial Infections↗

[Antibody binding bacteria in the urine in chronic pyelonephritis].

Using the direct immunofluorescence technique antibody-coated bacteria were demonstrated in urine samples from 49 of 57 patients with the clinical diagnosis of chronic pyelonephritis, but were not observed in urines from 11 patients with cystitis. A correlation rate was found between the presence of antibody-coated bacteria in the urine seidment and elevated serum antibody titers in the pyelonephritis group as determined by the indirect immunofluorescence technique. Patients with elevated serum antibody titers without antibody-coated bacteria in the urine, and vice versa, were also found. Patients with cystitis did not have elevated serum antibody titers against the homologous strain isolated from the urine. The clinical diagnosis of chronic pyelonephritis could be confirmed in 86.5% of patients taking into account the presence of antibody-coated bacteria and/or elevated serum antibody titers.

Adult↗

Long-term study of humoral immune response in patients with chronic pyelonephritis.

Indirect immunofluorescence was used for the measurement of serum antibody titers against the hemologous urinary strain of infecting organism in patients with a clinical diagnosis of chronic pyelonephritis. The passive hemagglutination technique was employed for comparison. The antibody response of the patients was followed-up for a period of two to four years. In contrast to titers determined by hemagglutination, antibody titers determined by immunofluorescence were found to be persistently elevated, and the indirect immunofluorescence technique is considered a sensitive and reliable method for the serological diagnosis of chronic pyelonephritis.

Anti-Bacterial Agents↗

[Indirect immunofluorescence in the serological diagnosis of chronic pyelonephritis (comparison with indirect haemagglutination and the Widal reaction (author's transl)].

Using indirect immunofluorescence, o-antibodies against the homologous causative organism were determined in 119 adults with chronic pyelonephritis, during a one to two-and-a-half year period. Humoral antibodies were determined in the same group of patients with the Widal reaction and indirect haemagglutination. The results were compared with those in a group of adults without renal disease, using the same serological reactions. In adults with chronic pyelonephritis the antibody titre (by immunofluorescence) was above normal limits (as obtained in the healthy group) in 63.1%. The corresponding results for the Widal reaction and indirect haemagglutination reaction were 16.5% and 12, respectively. Indirect immunofluorescence is thus clearly better than the other two serological methods in the diagnosis of chronic pyelonephritis.

Antibodies↗

[Serogrouping of Escherichia coli from urinary tract infections (author's transl)].

Serological grouping with 44 anti-O-sera was performed on 215 E. coli strains isolated from urine of patients with significant bacteriuria. 95 strains (44.2%) were typable, 34 strains (15.8%) proved to be rough strains, and 86 strains (40%) were untypbale. The O-groups 2, 6, 9 and 75 were found to be more frequent in patients with chronic pyelonephritis. Among pregnant women with asymptomatic bacteriuria a typical frequency was not observed.

Adult↗