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Biomedical subjects

E Busch

Publications and source records attributed to E Busch.

45 records · Page 3Linked to original sources
PubMed1991

[Diabetic examination in general practice. Screening of a practice population and a study of occurrence].

In a general practice, a screening examination for diabetes mellitus by measuring blood glucose in capillary blood was performed. A total of 2,843 persons aged 20-80 years were invited, 1,973 (69.4%) participated. At screening, 66 possible cases (BG greater than or equal to 8 mmol/l) were found. Among these, 16 diabetics were found, nine of whom had predisposing conditions. The prevalence of diabetes mellitus in the examined and known population of the practice was found to be 2.5% and calculated to be 2.2% in the total population of the practice. It was demonstrated that an investigation of this kind can be carried out but felt laborious and interfered with the daily work. Nevertheless, hitherto unknown diabetics were detected and instructed. A method of finding diabetics in general practice is thus described. The method is probably effective, as the prevalence of diabetes mellitus reached the expected, when the known and newly found diabetics were added.

Adult↗
PubMed1991

Fluorescent probes as tools to assess the receptor for the urokinase-type plasminogen activator on tumor cells.

Flow cytofluorometric protocols (FACScan) are described for the rapid and quantitative real-time analysis of binding of FITC-pro-u-PA to cell surface receptors (u-PAR) on living, resting, and also on PMA-stimulated human monocytic U937 cells. Binding of pro-u-PA was visualized by CLSM. This fairly new technique is superior over conventional fluorescence microscopy and is an alternative to electron microscopic approaches. Both flow cytofluorometry and confocal laser scanning microscopy allow the analysis quantitatively and with high-sensitivity binding of FITC-pro-u-PA to single suspended or adherent cells. By CLSM u-PA/u-PAR were found to be located in heterogeneously distributed discrete patches at the cell surface on U937 and not inside the cell. This is in agreement with previous studies by Hansen et al, who applied radioiodinated u-PA and electron microscopy to locate u-PAR on microvilli of fixed U937 cells. By flow cytofluorometry, it was possible to quantify the time-dependent and temperature-dependent binding of FITC-pro-u-PA to living single U937. Apparent saturation of u-PAR was achieved at 5 nM FITC-pro-u-PA for both nonstimulated and PMA-stimulated U937 cells. Half saturation of u-PAR was also determined. Nonstimulated U937 was 0.7 nM, and PMA-stimulated U937 was 1.1 nM of FITC-pro-u-PA. This increase in half-saturation concentration in PMA-stimulated cells is paralleled by a steep increase in binding sites (3.6-fold). The use of fluoresceinated reference beads is recommended to verify changes in affinity and binding sites. Using CLSM or flow cytofluorometry, it is also possible to study the structure relationship of u-PA/u-PAR in the presence of competitive binding analogues or inhibitors. Fluorescence techniques will also permit the identification of u-PAR-positive cells in blood, ascitic fluid, or biopsies obtained from cancer patients.

Amino Acid Sequence↗
PubMed1991

Intrapleural tetracycline for spontaneous pneumothorax in acquired immunodeficiency syndrome.

Spontaneous pneumothorax is occurring in patients with the acquired immunodeficiency syndrome and Pneumocystis carinii infection with increasing frequency. These patients are typically poor surgical candidates. Conservative management using tetracycline sclerosis was performed with good results in a patient with acquired immunodeficiency syndrome and recurrent pneumothorax.

Acquired Immunodeficiency Syndrome↗
PubMed1990

Prognostic scores in oesophageal or gastric variceal bleeding.

Numerous scoring systems have been developed for the prediction of outcome of variceal bleeding; however, only a few have been evaluated adequately. The object of this study was to improve the classical Child-Pugh score (CPS) and to test other scores from the literature. Patients (n = 82) with endoscopically confirmed variceal bleeding and long-term sclerotherapy were included in the study. Linear logistic regression (LR) was applied to different sets of prognostic variables with regard to 30-day mortality. In addition, scores from the literature were evaluated on the data set. Performance was measured by the accuracy and receiver-operating characteristic curves. The application of LR to all five CPS variables (accuracy, 80%) was superior to the classical CPS (70%). LR with selection from the CPS variables or from other sets of variables resulted in no improvement. Compared with CPS only three scores from the literature, mainly based on subsets of the CPS variables, showed an improved accuracy. It is concluded that CPS is still a good scoring system; however, it can be improved by statistical analysis using the same variables.

Esophageal and Gastric Varices↗
PubMed1984

[Hemorrhaging in early pregnancy].

283 patients with vaginal bleedings in the first three months of pregnancy were included in an analysis and compared to a control group. We could not find a correlation between bleeding in the early weeks of pregnancy and the age of the mother, the onset of menarche, parity, and preceded premature deliveries. On the other hand we found a significantly high number of bleedings in women, who already had abortions before, and diseases during pregnancy (infections, hyperemesis gravidarum). No statistical differences could be discovered in relation to the control group referring to the course of labour (rupture of membranes, changes of the amniotic fluid, operative deliveries, obstetrical presentation, first and second stage of labour, and complications of the delivery). The average birth weight was significantly lowered because of the large amount of premature births. We could not prove any increase of the rate of deformity after bleeding in the early weeks of pregnancy.

Adult↗