[Prevalence of sheep toxoplasmosis determined by the Sabin-Feldman reaction in animals from Uruguaiana, RS, Brazil].
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Biomedical subjects
Publications and source records attributed to E C Guimarães.
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In 80 apparently healthy pregnant women and their newborns tested for toxoplasmosis through the dye-test (RSF) greater than or equal to 1:16, were found respectively 50% and 38.7% positives. The positive mothers had positive newborns. The major part with the same titers as their mothers or one titer lower. The negative mothers had negative healthy newborns with one exception, considered "false positive reaction". The great majority of positive newborns were found negative at the 8th month, and the other until the 12th month. It's emphazised that the maternal and the newborn serology must be done at the same time. This considerations should be remembered when asymptomatic newborns with positive serology for toxoplasmosis are discussed.
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UNLABELLED: Some improvements on laboratory technics for Toxoplasmosis are presented: 1--the use of serum from guinea-pigs as accessory factor (AF) on the dye-test; 2--the use of filter-paper discs for collecting and storing blood of children and small animals for the dye test; 3--conservation of Toxoplasma gondii at various media and temperatures. RESULTS: 1--the pool of serum from 14 guinea-pigs gave good results when diluted in 1/5 Alsever solution at the proportion of 6/10; 2--the blood from digital puncture usually could be collected on filter-paper discs of 15 mm of diameter and stored at the freezer to -23 degrees C for 3-4 weeks, protected with alumen-paper. The dilution with saline 8.5% could be made 1/2 hour before starting the procedure; 3--the Toxoplasma gondii (cysts and trophozoites) could be stored at LIT medium or saline 8.5% at refrigator a +4 degrees C for 15-22 days, with some reduction in virulence.
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