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Biomedical subjects

E Cairns

Publications and source records attributed to E Cairns.

14 recordsLinked to original sources
PubMed1991

Multilocus and single locus minisatellite analysis in population biological studies.

In this review we describe the situations in which minisatellite analysis is of value to studies of population and evolutionary biology. Evolutionary and population biologists need to be able to quantify genetic relationships among individual organisms at many different levels, from close familial relationships to evolutionarily distant phylogenetic ones. The use of minisatellite markers is put into this context and compared with the other molecular biological techniques. Examples of the use of multilocus minisatellite analysis in population biology are described. The limitations of multilocus fingerprinting are presented, together with the potential advantages of locus-specific probes. The use of locus-specific probes in population biology is now often feasible due to the recent development of a cloning system which allows their efficient isolation. The availability of locus-specific probes should significantly expand the role of minisatellite markers in population biology.

Animals↗
PubMed1991

Northern Ireland: political violence and self-reported physical symptoms in a community sample.

In order to investigate the possible relationship between physical health and political violence in Northern Ireland a random sample of residents of four electoral areas (two with relatively high violence and two with relatively low violence) was interviewed at home. Each person was asked to rate their health in terms of common physical symptoms, to indicate their use of family doctor and hospital services, and to rate the level of political violence in their neighbourhood. Analysis of covariance (with a measure of psychological well-being, a measure of trait neuroticism plus age and socioeconomic status as covariates) revealed that women reported more physical symptoms than did men, people in the 'high' violence areas reported more symptoms than did those in the 'low' violence areas, while those who rated their own neighbourhood most highly in terms of perceived violence also reported the greatest number of physical symptoms. However, a series of chi 2 tests revealed no association between political violence or perceived political violence and uptake of services.

Cross-Sectional Studies↗
PubMed1991

The development of psychological well-being in late adolescence.

Studies following adolescents from school to work indicate a sharp decrease in psychological symptoms over the 16-17-year period compared to a more gentle decrease subsequently. This could be a genuine developmental phenomenon, related to school leaving, or because anxiety levels are higher at age 16 years due to approaching examinations. The present study examined these hypotheses. GHQ scores for those at school were related to examination candidacy. Eighteen months later such differences had disappeared among those young people now in full-time employment. Similarly, a group who remained in full-time education also showed a significant decline in GHQ scores.

Adaptation, Psychological↗
PubMed1990

Comparative study of idiotypes on monoclonal antibodies derived from patients with lupus and leprosy and from normal individuals.

A collaborative study was performed to compare the expression of a series of idiotypes defined on human anti-DNA and other autoantibodies. Three panels of human monoclonal antibodies were tested: eight derived from patients with systemic lupus erythematosus (SLE); 13 from an individual with lepromatous leprosy; and 38 from normal subjects. The following rabbit anti-idiotype sera were used: one (RId16/6) raised against the lupus-derived monoclonal anti-DNA antibody 16/6, four (RId8E7, RId4G7, RId4D5 and RIdTH9) against leprosy-derived monoclonal antibodies of various specificities, and one (anti-4.6.3) against a normal-derived anti-DNA monoclonal (KIM 4.6). In addition, two other anti-idiotypes were used--one a murine monoclonal (3I), the other a rabbit polyclonal (RIdD)--which had been raised against polyclonal anti-DNA antibodies from lupus serum. Further experiments were performed with immunoabsorbed fractions of RId8E7. Direct-binding and competition assays were used. All of the anti-idiotypes produced different patterns of positivity among the three panels of human monoclonal antibodies, with the exception of RId8E7 and RId4G7, which showed considerable concordance. There was a tendency towards anti-idiotypes being disease- or group-specific: thus anti-4.6.3 failed to bind to any of the lupus or leprosy-derived monoclonals, while RId16/6 and RId8E7 bound most strongly to the lupus- and leprosy-derived antibodies respectively. KIM 4.6 itself was bound only weakly by RId16/6, while 16/6 was not recognized by anti-4.6.3; 16/6 was, however, bound by 3I, while KIM 4.6 was not. 3I bound to several other monoclonals but RIdD, which has been shown to be specific for the anti-DNA fraction of lupus serum, did not bind to any of them. These results indicate that the majority of these anti-idiotype preparations recognize largely separate sets of determinants. The monoclonal antibodies which bind to DNA may be only partly representative of anti-DNA antibodies in the serum of lupus patients.

Antibodies, Anti-Idiotypic↗
PubMed1990

Impact of television news exposure on children's perceptions of violence in Northern Ireland.

The present study assessed the impact of television news exposure on Irish children's perceptions of the level of violence in their neighborhoods. Estimates from children (N = 570) of the local levels of violence in three areas (high-, low-, and no-violence) were subjected to a four-way analysis of variance (Area x Sex x Age x News Exposure) that showed a main effect for area and sex with respect to the high-violence area and boys. Two two-way interactions also reached statistical significance. The first suggested that the perception of lower levels of violence by girls was confined to the high- and low-violence areas. The second concerned the level of actual violence and the amount of reported news exposure. Only in the high-violence area were these two variables related: Accurate estimates of actual violence levels were associated with more frequent news exposure.

Child↗
PubMed1990

A human anti-cardiolipin autoantibody is encoded by developementally restricted heavy and light chain variable region genes.

Based on recent structural analyses of monoclonal autoantibodies, it appears that a number of these antibodies express germ-line immunoglobulin variable region (V) genes with little or no somatic mutation. In addition, our group and others have noted the identity or near identity of some autoantibody-associated V genes to V genes apparently expressed preferentially in the fetal pre-B cell repertoire. To extend these data, we now report that the heavy and light chain V genes of an anti-cardiolipin antibody derived from a healthy individual display 99% nucleotide sequence homology with V genes expressed in early B cell ontogeny. Sequence comparisons indicate the likely use of fetal-restricted V genes by this autoantibody. Taken together with other data on autoantibody V gene usage, these findings provide further evidence for overlap between the autoantibody-associated and early ontogeny expressed V gene repertoires and suggest that natural autoreactivity may be instrumental in the development and maintenance of the normal immune repertoire.

Amino Acid Sequence↗
PubMed1979

Concurrent validity of a children's version of the Stroop Color-Word Test: the Fruit Distraction Test.

To assess the concurrent validity of the Fruit Distraction Test, the Stroop Color-Word Test and the Fruit Distraction Test were administered to two groups of boys aged 12 yr. (N = 63) and 8 yr. (N = 52). Partial correlations, with IQ controlled, based on time and error scores derived from both tests, provided no convincing evidence that the Fruit Distraction Test is a valid downward extension of the Stroop.

Child↗
PubMed1978

Translation of tobacco mosaic virus RNA In Acetabularia cell cytoplasm.

Isolated Acetabularia nuclei were microinjected with Tobacco Mosaic Virus RNA and then implanted into an anucleate posterior fragment of an Acetabularia cell. Injected RNA was translated in the Acetabularia cytoplasm from the first to twelfth day after implantation of the nuclei. The production of specific virus proteins was detected and localized in the Acetabularia cytoplasm by an immunofluorescence precipitation technique.

Acetabularia↗
PubMed1978

Translation of animal virus RNA in the cytoplasm of a plant cell.

Isolated Acetabularia crenulata nuclei were injected with Mengo virus RNA solution and then implanted into anucleate posterior Acetabularia mediterranea cell fragments or fused with Acetabularia ryukyuensis cytoplasts. The injected animal virus RNA was actively translated in the plant cell cytoplasm. Mengo virus proteins were detected and localized in Acetabularia cytoplasts by use of an immunofluorescence method on the first to fifth day after injection.

Acetabularia↗
PubMed1978

Role of task strategy and stimulus processing in reflective and impulsive tactual matching performance.

14 reflective and 14 impulsive boys performed tactual matching tasks in which frequency of observation of the standard was manipulated by successive or simultaneous presentation and in which stimulus features were controlled so that the amount of stimulus analysis carried out might be inferred. The results suggest that an impulsive performance was improved by simultaneous presentation of the stimuli but even in this condition the impulsive subjects did not carry out an adequate amount of stimulus analysis.

Child↗