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E Carasevici

Publications and source records attributed to E Carasevici.

At least 37 records · Page 2Linked to original sources
PubMedDate not supplied

[High values of soluble cytokeratin 18 in breast carcinomas may have different meanings].

We have investigated the cellular and serum CK18 in 26 non-treated primary ductal invasive breast carcinomas. The soluble CK18 (TPS) was detected by chemiluminescent assay, and the cellular CK18 and PCNA expression by immunocytochemistry. Flow-cytometry was used to estimate the amount of DNA in malignant cells. There was a significant correlation between soluble CK18 and the pre-menopausal status (p < 0.05), characterized in our group by a PCNA estimated low proliferation index. We have also found a significant correlation between soluble CK18 and the DNA index (p < 0.01). The intracellular CK18 has correlated with the PCNA expression (p < 0.05), while no correlation could be found between cellular and serum CK18. The values of soluble CK18 may offer information about the treatment-induced cell death, if monitored, while isolated measurements should be interpreted cautiously. Elevated levels of serum CK18 in non-treated carcinomas may rather reflect a high tumor turn-over or perhaps a more intensive tumor cell killing.

Biomarkers, Tumor↗
PubMedDate not supplied

[The correlation between the clinical stage and multimodal treatment in breast cancer expressed in the 5-year survival rate].

The survival rate in 143 patients with breast cancer followed up for 60 months has been evaluated by the regression Cox method and life table method, a number of representative variables for tumor and/or host being taken into account. The Cox model coefficients pointed out the following factors to positively influence the survival: age, premenopausal status, surgery, radiotherapy and complete chemotherapy (6-12 cycles). Negative and highly negative values recorded within variables: tumor over 5 cm, the presence of metastases, Karnofsky index less than 80 and the postmenopausal status generally correlated with the disease in advanced clinical stages. An apparently better survival in patients who underwent radiotherapy as compared to those who underwent an associated radiochemotherapy could be the result of the preferential associated treatment in advanced stages. The optimal intensity of chemotherapy positively influences the survival, except in late stages, suggesting the necessity of chemotherapy in initial stages.

Age Factors↗
PubMedDate not supplied

Histoenzymological and ultrastructural changes of axillary lymph nodes with breast cancer metastases.

Histoenzymatic and ultrastructural changes were studied in axillary lymph nodes from patients with breast cancer by comparison between lymph nodes without metastases, with incipient metastases and completely metastatically transformed. Metastases is preceded by intense macrophagic reactions of lymph nodes, and prepared by the development of vascular stroma. Neoplastic cells in course of invasion presented an intense respiratory activity, while those replacing the lymphoid elements an accentuated synthesis of glycoproteins.

Acid Phosphatase↗
PubMedDate not supplied

[The parameters of the immune function in the evolution of HIV-1 infection in institutionalized children in Romania].

OBJECTIVE: To describe the evolution of HIV-1 + horizontal infected children nursed in closed community. METHOD: The biological status of 26 HIV-1 + children nursed in Iaşi Orphanage was assessed in dynamic during April. 1993-Feb. 1994. The income age ranged among 1 month-3 years. RESULTS: The following progressive stages could be drawn accordingly to the biological status parameters dynamic: stage 1 ("oligosymptomatic"), stage 2 ("multiform, medium or severe symptomatology"), and stage 3 ("severe immunodepression, with predominant infectious symptomatology, waves evolution"). These stages could not be assimilated to the currently CDC or WHO classifications. The following thresholds of the immunologically parameters separate the stages 1 and 2, and respective stages 2 and 3: CD4% lymphocytes (27% respective 20%); absolute CD4+ lymphocytes (1150/microliter respective 700 + 750/microliter), CD4/CD8 ratio (0.75 respective 0.45), beta 2-microglobulin (1.5 mg/1000 respective 2.5 mg/ 1000). Lymphocytes lacking the markers CD4, CD8, CD19, CD3, ranging between 15 + 20% were also detected by flow-cytometry; these cells could be attributed to the immature subpopulations (typically for dystrophy) or to down-regulation of membrane expression of some markers. Also, a dichotomy in the distribution of the CD2/CD3 surface markers was recorded in the case of lymphocytes. CONCLUSIONS: Immunological features demarcate this epidemiological group versus the current described models for the HIV-1 + child.

Biomarkers↗
PubMedDate not supplied

An immunohistochemical study of P53 protein on colorectal carcinomas.

AIMS: To investigate the frequency of immunohistochemical expression of p53 protein in colorectal carcinomas; to evaluate the correlation between p53 alterations and the histopathological parameters; to determine the prognostic significance of this tumor marker. PATIENT POPULATIONS: Fourty patients with colorectal carcinomas. METHODS: Paraffin embedded normal and tumoral tissues, stained, with HES, PAS, PAS-Alcian Blue stains. For detecting the p53 protein, a three step (streptavidin-biotin immunoperoxidase) immunohistochemical method was used. RESULTS: 28 out of 40 (70%) colorectal carcinomas were positive for p53. The identification of nuclear accumulations of p53 protein in the present study was significantly correlated with the anatomic site and tumor stage. CONCLUSIONS: The mutational pattern observed in this study was substantially as expected. The p53 overexpression is present in advanced stage, thus demonstrating the prognostic value of this marker.

Adenocarcinoma↗
PubMedDate not supplied

[The treatment of rectal cancer--the optimizing possibilities].

The authors studies 280 rectal cancers treated in the first surgical clinic of Iaşi in the period 1981-1994. Although surgery is the main treatment of these tumors, it cannot be and it must be not used alone. Surgery must be associated with radio- and chemotherapy, because is the only way possible to increase the number of resections for rectal tumors and the survival rate. For the 263 tumors operated the resectability was 73, 5%: Milles's operation 101 (36, 7%), Dixon's operation 81 (30, 79%), other operation 91 (33, 11%). We consider that Dixon's operation is the best choice for the tumors in the stages A and B of Dukes classifications. Preoperative radiotherapy can influence the tumor volume (downstaging) and decrease local recurrences to about 10-15%. Surgery alone must be practiced only in emergencies. Postoperative chemotherapy is mandatory and it reduces local recurrences and delays metastasis evolution.

Adult↗
PubMedDate not supplied

Flow-cytometry DNA index of colorectal carcinomas.

The authors investigated the relationship between flow cytometric DNA index (D.I.-defined as the ratio of the DNA content of malignant cells to that of normal cells) and other prognostic factors (grade, stage of the disease, anatomical site, patients' sex and age) of 20 patients with colorectal cancer. The entire material was obtained from 20 unselected and previously untreated patients. This material consists of fresh tumoural or normal tissues taken after radical colectomy for the flow-cytometric analysis. A normal diploid DNA content was found in six tumours, the other fourteen being founded to be aneuploid. All non malignant tissue samples showed normal diploid DNA content. In the current series of cases we have found that DI was significantly associated with stage but not with grade, anatomical site or other prognostic factors. Our data indicates that cytometrically evaluated DI values have probably an independent predictive power for the clinical outcome of colorectal cancer patients.

Adult↗