[Cytologic study of the peritoneal fluid by puncture of Douglas' pouch].
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Biomedical subjects
Publications and source records attributed to E Cava.
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Female Albino Rats exposed to permanent light, have polycystic and anovulatory ovaries, with a vaginal cytology composed of cornified cells (persistent estrus) and a plasmatic hyperandrogenia. These structural modifications are similar to those of women affected by Stein-Leventhal's syndrome. The possible role of regulation of the pineal gland is considered.
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In vaginal exfoliative cytology, the routine Papanicolaou and Shorr's staining procedures do not reliably indicate the post-ovulatory phase. An experimental study of 100 female albino rats and a clinical study of 190 women was initiated to establish an easier method for identifying the post-ovulatory phase. It was found that the appearance of intracytoplasmic lipid granules in vaginal cells stained by Oil Red 0 may serve as a sensitive indicator of the post-ovulatory phase.
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A cytocentrifugation technique for the preparation of monodispersed preparations of bronchial cells collected by brushing is described. First developed with the aim of obtaining slides suitable for automated analysis, it was found to be useful for routine morphologic examinations. This technique requires careful preparation as well as "education" of the pathologist/screener in the appearance of the cellular elements in its samples. However, it is reliable and reproducible and, above all, eliminates a certain number of artifacts found in conventional preparations.
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