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Biomedical subjects

E Copeland

Publications and source records attributed to E Copeland.

12 recordsLinked to original sources
PubMed2000

Inhibition of 1,2-dimethylhydrazine-induced oxidative DNA damage in rat colon mucosa by black tea complex polyphenols.

The effect of black tea polyphenols on 1,2-dimethylhydrazine (DMH)-induced oxidative DNA damage in rat colon mucosa has been investigated. Fischer 344 rats were treated orally with thearubigin (TR) or theafulvin (TFu) for 10 days (40 mg/kg), injected ip with DMH (20 mg/kg) or saline and sacrificed 24 hr after DMH administration. The levels of 8-hydroxy-2'-deoxyguanosine (8-OHdG) were measured in colonic mucosa DNA and expressed as a ratio relative to 2'-deoxyguanosine (2dG). Control rat mucosa had 8-OHdG values of 1.12 +/- 0.14/10(5) dG (mean +/- SEM, n=11), whereas DMH-treated rats significantly higher values (1.52 +/- 0.14/10(5) dG, n=26, P<0.05). Pretreatment of rats with TR had significantly inhibited DMH-induced oxidative DNA damage 0.99 +/- 0.09/10(5) dG, n=10, P<0.05) and a similar, although less marked, effect was observed with TFu (1.15 +/- 0.19/10(5), n=9, P=0.06). These findings confirm that DMH causes oxidative DNA damage in the colon mucosa of rats and demonstrate that this effect is prevented by the consumption of complex polyphenols from black tea.

1,2-Dimethylhydrazine↗
PubMed1999

Proliferation of hepatic peroxisomes in rats following the intake of green or black tea.

Rats maintained on green, black or decaffeinated black tea (2.5%, w/v) as their sole drinking fluid displayed higher hepatic CN- insensitive palmitoyl CoA oxidase activity than controls; the extent of increase was similar with the three types of tea. Morphological examination of the liver using electron microscopy revealed an increase in the number of peroxisomes in the tea-treated animals. The same treatment of the animals with green and black tea resulted in a similar rise in hepatic microsomal lauric acid hydroxylation. Analysis by HPLC of the aqueous tea extracts employed in the current study showed that the total flavanol content of the green variety was much higher than the black varieties, and confirmed the absence of caffeine in the decaffeinated black tea. It may be concluded from the present studies that neither caffeine nor flavanoids are likely to be responsible for the proliferation of peroxisomes observed in rats treated with tea.

Animals↗
PubMed1998

Contribution of theafulvins to the antimutagenicity of black tea: their mechanism of action.

Theafulvins were isolated from black tea aqueous infusions and their antimutagenic activity was evaluated against a number of food carcinogens. Theafulvins gave rise to a concentration-dependent inhibition of the mutagenicity of 2-amino-3-methylimidazo-[4,5-f]quinoline, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine, benzo[a]pyrene, 7,12-dimethylbenz[a]anthracene, nitrosopyrrolidine and nitrosopiperidine, but, in contrast, the mutagenicity of aflatoxin B1 was enhanced. The mutagenicity exhibited by N'-methyl-N'-nitro-N-nitrosoguanidine and 9-aminoacridine was not influenced and weakly inhibited by theafulvins, respectively. The p-hydroxylation of aniline and the O-dealkylations of methoxy-, ethoxy- and, to a lesser extent, pentoxyresorufin were inhibited by theafulvins in a concentration-dependent manner. When microsomal metabolism was terminated after metabolic activation of the promutagens, incorporation of the theafulvins into the activation system did not modulate the mutagenic response. It is concluded that theafulvins play an important role in the antimutagenic activity of black tea by inhibiting cytochrome P450-dependent bioactivation of the carcinogens.

9,10-Dimethyl-1,2-benzanthracene↗
PubMed1992

The effects of glutamine-enriched total parenteral nutrition on tumor growth and host tissues.

The effects of glutamine-enriched total parenteral nutrition (TPN+GLN) were studied in tumor-bearing rats because glutamine can benefit host tissues but also may stimulate tumor growth. Rats were implanted with the methylcholanthrene-induced fibrosarcoma (MCA sarcoma) and were studied when the tumor constituted less than 5% of carcass weight (small tumor) and when the tumor constituted 10% of carcass weight (large tumor). Provision of 20% of TPN protein as glutamine produced a significant increase in the arterial glutamine level and maintained the skeletal muscle intracellular glutamine concentration (2.02 +/- 0.1 versus 1.39 +/- 0.07 mumol/g, p less than 0.01). Concurrently, hindquarter GLN fractional release increased nearly threefold (p less than 0.05) in the TPN+GLN group. Glutamine-enriched total parenteral nutrition did not affect carcass weight, tumor weight, tumor DNA content, or tumor glutaminase activity. Furthermore, DNA flow cytometric analysis did not demonstrate any difference in percentage of aneuploid tumor cells within the G1, S, or G2M cell cycles. However, the ratio of aneuploid to diploid cells within the tumor mass increased by 20% in animals receiving glutamine. Glutamine-enriched total parenteral nutrition had no effect on tumor glutathione (GSH) levels. No increase in hepatic GSH levels was observed, but gut mucosal GSH levels were 20% greater in the TPN+GLN group (p less than 0.05). The provision of glutamine-enriched TPN may be beneficial to the host by maintaining skeletal muscle glutamine stores and by supporting gut GSH biosynthesis. In this tumor model, TPN+GLN does not appear to increase tumor size, tumor DNA content, or tumor glutamine metabolism, but the ratio of tumor cells to host infiltrating cells within the tumor mass appears to be increased.

Animals↗
PubMed1973

Equilibrium potentials of membrane electrodes.

A simple thermodynamic theory of the equilibrium potentials of membrane electrodes is formulated and applied to the glass electrode for measurement of pH. The new formulation assumes the selective adsorption or binding of specific ions on the surface of the membrane which may or may not be permeable to the ion, and includes the conventional derivation based on reversible ion transport across membranes as a special case. To test the theory, a platinum wire was coated with a mixture of stearic acid and methyl-tri-n-octyl-ammonium stearate. When this coated electrode was immersed in aqueous phosphate solution, its potential was found to be a linear function of pH from pH 2 to 12 with a slope equal to the theoretical value of 59.0 mV per pH unit at 24 degrees .

Biological Transport↗
PubMed1966

Salt transport organelle in Artemia salenis (brine shrimp).

The branchiae of Artemia adapted to triple-strength sea water (105 per mil salinity) were studied with the electron microscope. The epithelial lining of the metepipodite segment possesses organelles composed of stacks of disc-shaped mitochondria interlaced with flattened extensions of a canalicular system that in turn communicates with the plasma-bound surface of the cells. The distance between the canalicular and mitochondrial membranes is small and quite constant. The marked similarity to "mitochondrial pumps" in the anal papillae of tosquito larvae suggests that the organelle is concerned with salt transport.

Animals↗