PubMed Health⌕ Search

Biomedical subjects

E Cortes

Publications and source records attributed to E Cortes.

34 records · Page 2Linked to original sources
PubMed1987

Improved method for quantifying vitamin D in proprietary infants' formulas and in breast milk.

In this new method for quantifying vitamin D in infants' formulas and breast milk, after repeated lipid extraction, samples are further purified by passage through Sep-Pak cartridges, followed by liquid chromatography, then quantified by competitive protein-binding assay. Analytical recovery is estimated by use of added 3H-labeled vitamin D. For repeated assays of a reconstituted proprietary powdered milk formula in two runs, the intra-assay vitamin D values were 9.0 (SD 1.4) (n = 6) and 8.9 (SD 1.6) micrograms/L (n = 7) (t = 0.07). Assay of a proprietary liquid formula yielded values of 14.9 (SD 0.9) micrograms/L (n = 6). For each, the results agreed with the vitamin D content shown on the label. Vitamin D concentrations in breast milk from two groups of lactating mothers of different social class and nutritional status were 2.3 (SD 1.4) (n = 7) and 2.0 (SD 1.7) micrograms/L (n = 7). Overall recoveries ranged from 65 to 75%. Only 2 mL of milk is required, which facilitates sample collection, and the assay is less time-consuming than other current methods.

Chromatography, Liquid↗
PubMed1986

Metastatic trichomatricial carcinoma.

Trichomatricial carcinoma has been recently recognized as a rare malignant variant of pilomatricoma with a potential for local recurrence and, rarely, for distant metastases. We describe such a tumor in a 52-year-old man who developed local recurrence and axillary lymph node metastases within six months of local excision, followed by bilateral pulmonary metastases and 2 1/2 years later by death due to disseminated tumor. This, to our knowledge, is the second reported case of metastasizing trichomatricial carcinoma.

Carcinoma↗
PubMed1985

Effective multimodality treatment for advanced epidermoid carcinoma of the female genital tract.

Fifteen patients with advanced or recurrent squamous-cell carcinoma of the cervix, vulva, vagina, and urethra were treated with simultaneous combination chemotherapy (5-fluorouracil infusion and mitomycin C) and radiotherapy (3,000 rad for a period of three weeks). Three to four weeks after completion of radiotherapy, 13 of 15 patients achieved partial or complete tumor shrinkage. Nine of 15 patients are alive, eight of whom (at a median follow-up time of 24 months) have no evidence of disease. The longest survival time was 45 + months. There was minimal toxicity associated with this therapy. The results of this pilot study suggest that the simultaneous administration of radiation and chemotherapy is an effective method of treatment of advanced female genital tract carcinoma.

Adult↗
PubMed1985

Citrate determination with aconitase immobilized on solid support.

Pig heart aconitase has been immobilized on Enzacryl AA solid support and its kinetic behaviours were studied by using a stirred bath reactor with continuous recycling. In this reactor, the best flow rate has been determined to eliminate diffusional problems. Kinetics constants, thermic stability and pH variations have been compared between the soluble and immobilized aconitase for determination of enzyme-Enzacryl AA effectivity. Stability of the soluble and immobilized aconitase was also studied after repeated use and long-time storage. While the soluble form loses its activity after 24 hr storage, the immobilized form preserves its full activity after repeated usage and long-lasting storage. Finally, an easily measurable parameter has been found to quantitate citrate. The maximum increase of absorbance, is proportional to citrate concentration in a range between 0.2 and 3.2 mM. In conclusion, these results show that the immobilized aconitase system can be used for the determination of citrate with reproductility and great sensitivity. In addition to the simplicity of the assay, great economy in enzyme consumption has been demonstrated, in contrast to the traditional methods of quantitative citrate analysis.

Aconitate Hydratase↗
PubMed1984

Incorporation of bovine thyroid peroxidase in liposomes.

In the microsomal fraction of thyroid glands, the temperature dependence of DPH fluorescence polarization showed a discontinuity in the range of 29-33 degrees C. The transition temperatures of DMPC, DPPC and DSPC are near to the observed for the microsomal fraction. So that, thyroid peroxidase (TPO) was incorporated into liposomes made with these phospholipids. When DPH was incorporated in this peroxidase-liposome complex, a less pronounced phase transition was observed in the profiles of temperature dependence of DPH polarization, and the incorporation of the enzyme decreased the Tc. Arrhenius plots of TPO incorporated into liposomes showed discontinuities at similar temperatures observed by fluorescence polarization. The decrease of transition temperature of liposomes induced by thyroid peroxidase incorporation suggests that this enzyme seems to need a fluid medium for its enzyme activity.

Animals↗
PubMed1984

A comparison of intermittent vs. continuous and of adriamycin vs. methotrexate 5-drug chemotherapy for advanced breast cancer. A Cancer and Leukemia Group B study.

The therapeutic effectiveness of intermittent vs. continuous combination chemotherapy and of the substitution of adriamycin for methotrexate in a 5-drug regimen was evaluated in women with metastatic breast carcinoma. Patients were randomly allocated to receive continuous therapy with cyclophosphamide, methotrexate, 5-fluorouracil, vincristine, prednisone ( CMFVP -C, 86 patients), intermittent CMFVP ( CMFVP -I, 109 patients), or intermittent CAFVP (107 patients). The CR + PR rate with CAFVP (71%) was superior to CMFVP -C (50%, p = 0.003) and to CMFVP -I (50%, p = 0.002). The remission duration with CAFVP (14 months, median) was superior to CMFVP -I (7 months) (p less than 0.01), and tended to be superior to CMFVP -C (9 months) (p = 0.07). There was a survival advantage of CAFVP (19 months, median) over CMFVP -I (13 months) (p = 0.01), but not over CMFVP -C (16 months) (p = 0.24). Among CR + PR patients, the survival with CAFVP (29 months, median) was superior (p = 0.02) to both CMFVP -I (18 months) and CMFVP -C (21 months). The CMFVP -C regimen was associated with the highest incidence of leukopenia and neurologic toxicity, but the lowest incidence of GI toxicity. The results indicate that the CAFVP regimen is well tolerated and is superior to the CMFVP regimens.

Age Factors↗
PubMed1981

Compartmental body composition of cancer patients by measurement of total body nitrogen, potassium, and water.

Quantitative measurement was made of body composition in patients with several forms of neoplastic disease. Total body nitrogen was determined by means of the prompt gamma neutron activation technique; total body potassium was measured with the use of a whole body counter. The mass and protein content of the muscle compartment and nonmuscle lean tissue were estimated by application of the technique of compartmental analysis. Total body water, determined simultaneously with the use of tritium label, provided a measure of lean body mass. From these data, the body fat can be inferred. The prompt gamma neutron activation and whole body counting techniques represent a considerable advance over the balance and radioisotope techniques used in earlier studies. The new techniques make possible sequential studies over prolonged periods of time with a considerable degree of accuracy. The loss of body weight by patients with solid tumors consisted primarily of the loss of muscle mass and body fat. Even in severe wasting, the patients appear to retain significant amounts of body fat. It is the skeletal muscle which is predominantly lost; the visceral life-supporting system is, to a considerable extent, spared. The nonmuscle tissue including the visceral fraction did not change in this study, and actually appeared to increase in size when comparison was made with the normal contrast population. The loss of total body water was slight in the cancer patients studied.

Adipose Tissue↗
PubMedDate not supplied

[Effects of 1,5 benzodiazepine beta lactam on sleep].

Various substances have been used as sleep inductors, but their use has declined, due to their undesirable side effects, and they have been replaced mainly by benzodiazepines. There currently exists a great variety of benzodiazepines with an increasing, clinical use, although they also present certain disadvantages. The effect on sleep of the intraperitoneal administration (0.9 mg/kg) of 7-(p-cl-phenyl)-8-phenoxy-4,5-benzo-3-aza-2-none, was analysed in chronically implanted wistar rats. Results indicate that this substance decreases wakefulness and increases significantly slow-wave and paradoxical sleep. Latency of the first phase of paradoxical sleep show a tendency to increase. It is concluded that this substance has hypnogenic effects.

Animals↗