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Biomedical subjects

E Csonka

Publications and source records attributed to E Csonka.

At least 37 records · Page 2Linked to original sources
PubMed1984

Examination of a spontaneously transformed aortic smooth muscle cell line. I. Morphological examinations.

Spontaneous transformation was observed of a mini-pig aortic smooth muscle cell line in its 21st passage in vitro. The transformed cells exhibited changed phenotype, heteroploidy and excessive proliferative activity. The initially elongated cells developed a polymorphous essentially polygonal appearance. The longitudinal filaments characteristic of normal cells disappeared and only finely granular double refracting matter was detectable in polarized light. The transformed cells exhibited microvilli in both transmission electron microscopic and scanning electronmicroscopic preparations. The appearance of spontaneous lectin binding in the transformed cells is a sign of altered plasma membrane structure. The possible contaminational origin of the transformed cells was carefully excluded.

Animals↗
PubMed1983

Mechanism of thrombin binding to endothelial cells.

The interaction of human alpha-thrombin with mini-pig aortic endothelial cells was studied using 125I-labeled enzyme. Equilibrium between bound and free thrombin was attained within 1 min, and the Klotz-Hunston equations indicated two populations of binding sites. Approximately 30,000 sites/cell belonged to the high-affinity class with a Kd of about 3 x 10(-8) M. Modification of two lysine residues of thrombin with pyridoxal 5'-phosphate (PLP2-thrombin) destroyed the high-affinity binding and about three-fourths of the low-affinity bindings. When the lysine residue of thrombin involved in heparin binding was protected with heparin against chemical modification (PLP-thrombin), the modified enzyme remained similar to the native one with respect to cellular binding, with some loss of low-affinity binding only. Heparin, in a tenfold molar excess to enzyme, inhibited the binding of the native as well as the PLP-thrombin, whereas it did not influence the interaction between PLP2-thrombin and the cell. Since heparin might interfere with both the enzyme and the cell, the binding of heparin to endothelial cells was also examined. The results revealed that 3H-heparin also bound to cells. This binding was characterized by a Kd of 3 x 10(-7) M, approximately 10(6) sites/cell. Furthermore, thrombin bound to endothelial cells was released by antithrombin III. On the basis of these and other data in the literature, a model is proposed for the mechanism of the binding of thrombin to endothelial cells.

Antithrombin III↗
PubMed1981

The synthesis, transport and excretion of connective tissue macromolecules by smooth muscle cells.

SMC-s both in vivo and in vitro have a function in matrix macromolecule production. Under certain conditions the synthesizing function of the SMC-s seems to be more important than their contractile function. The modified less differentiated SMC-s participate in the synthesis of connective tissue macromolecules while the differentiated "resting" SMC-s maintain the functional activity.

Animals↗
PubMed1981

The effect of chondroitin-sulfate and hyaluronic acid on glycosaminoglycan and collagen synthesis and secretion by cultured arterial endothelial cells.

The effect of the addition of chondroitin-sulfate or hyaluronic acid to the culture medium on the glycosaminoglycan and collagen content and on the glycosaminoglycan composition of pig arterial endothelial cells and of the culture medium has been studied by biochemical and morphological methods. Both chondroitin-sulfate and hyaluronic acid were found to be transported to the interior of the cultured cells and then degraded. CSA, in addition enhanced collagen synthesis within the cells, but not the rate of excretion. Hyaluronic acid did not influence collagen formation.

Animals↗
PubMed1981

Age-related changes in the glycosaminoglycans and collagen of cultured pig endothelial cells.

Pig aortic endothelial cells were grown from aortic intimal explants. Tissue culture samples were obtained at regular intervals between the 2nd and 21st day (time-course experiments) and between the 2nd and 15th passage (influence of subcultivation). Glycosaminoglycans (GAG-s) were isolated separately from the cells and from the medium. The various GAG-s were identified by electrophoresis on cellulose acetate strips, by differential degradations with HNO2 and by digestion with selective enzymes, and their concentration was measured. Protein and collagen content was also determined. Parallel tissue culture monolayers were investigated by microscopical methods. Total GAG and collagen concentrations showed maximum curves when plotted as a function of the time of incubation, with peak values on the 9th day, both in the cells and in the medium. From among the individual GAG-s the amount of hyaluronic acid and chondroitin-sulfate increased gradually, while heparan-sulfate content changed in the opposite direction. Chondroitin was detectable only in the culture medium; its concentration decreased steeply until the tenth day. Alterations in the dermatansulfate content showed inverse tendency. The production and excretion of collagen and of GAG-s increased during the first six passages, both in the cells and in the media, and then declined during the four following ones. The ratio of the individual GAG-s did not alter until the tenth subculture. Further subcultivation resulted in a gradual increase in the relative amount of chondroitin-sulfate and a decrease in heparan sulfate contents.

Aging↗
PubMed1980

Endothelial glycosaminoglycans-in vitro studies.

Endothelial cells were grown from pig aortic intimal explants. Collagen and glycosaminoglycan (GAG) content and GAG distribution was measured in the cells and in the culture medium as a function of the time on incubation and of subcultivation. The influence of the addition of chondroitin-6-sulfate or hyaluronic acid to the medium has also been studied.

Animals↗
PubMed1980

Morphological examination of aortic endothelial and smooth muscle cells grown in vitro on collagen membranes.

Collagen membranes were prepared from acid soluble collagen gels reconstituted by ammonia vapour. The membranes were seeded with pig aortic endothelial and smooth muscle cells. After few days confluent monolayers were obtained. By subsequent seeding it was possible to produce gel-sheets covered on one side by endothelium and on the other by smooth muscle cells. The transmission electron-microscopic picture of the cells grown on collagen membranes were not very different from those growing on glass. By scanning electron-microscopy however considerable difference was found between the morphology of cells growing on collagen or on glass.

Animals↗
PubMed1979

Measles virus induced lectin binding in cultured, aortic cells.

Using the indirect hemadsorption test it was shown that cultured diploid cells of mini-pig, monkey or human aortic origin develop wheat germ lectin/WGA/binding capacity following infection with measles virus. Heteroploid cell lines of similar origin have bound WGA also without being infected with virus. The possible role of virus induced membrane changes in vascular cell pathology is suggested by the results presented.

Acetylglucosamine↗
PubMed1978

Chromosome changes in subacute sclerosing panencephalitis.

In two out of a total of 6 cases of subacute sclerosing panencephalitis with manifest chromosome alterations, chromosome studies were repeated 4 and 5 years of standing, respectively, after manifestation of the disease. In one case the incidence of chromosome alterations increased while in the other no change was found. Both cases were clinically similar in their history with remission.

Antibodies, Viral↗
PubMed1977

Scanning electron microscopic examination of in vitro cultured cells by different methods.

1. The morphology of cultured aortic endothelial cells was studied by scanning electron microscopy. The cells were prepared for examination by three different procedures: two involved fixation, the third only drying in vacuum. 2. To check the efficiency of the methods of preparation, three non-endothelial cell lines were processes similarly and examined for SEM morphology. 3. The images of the cell surfaces were found to differ depending on the method of preparation and the value of the methodical approach clearly varied with the type of the cell. It is concluded that for studying cell morphology by SEM the method of preparation should carefully be selected.

Animals↗
PubMed1977

Influence of membrane active substances on cell surface morphology of cultured aortic endothelial cells.

1. The changes occurring on the surface of cultured aortic endothelial cells under the influence of exogenous cholesterol, lysolecithin, or cholesterol + lysolecithin were pursued by scanning and transmission electron microscopy. 2. Both compounds were found to cause surface changes under different quantitative relations, as shown by scanning electron microscopy of unfixed preparations. The changes were less significant in preparations fixed prior to the scanning--or transmission electron microscopic examination. 3. Other cell lines maintained in this laboratory and similarly treated with cholesterol, were found to be less responsive than the endothelial cells. 4. Cholesterol content was determined by thin layer chromatography in six different cell lines, before and after the addition of cholesterol. Only the endothelial cells showed a notable rise of cholesterol content after treatment. This fact may confirm our finding that cholesterol induced morphological changes were demonstrable only in the endothelial cells.

Aorta↗
PubMed1975

Imidazole in organs of inbred chickens selected for resistance or susceptibility to lymphoid leukosis or Marek's disease.

Total and soluble imidazole was determined on samples of liver and kidney and soluble imidazole was determined on blood from eight strains of chickens selected at three locations for resistance or susceptibility to lymphoid leukosis and/or Marek's disease. Liver and kidney total and soluble imidazole was not different between strains classed as resistant or susceptible to lymphoid leukosis and/or Marek's disease. The weighted average of soluble blood imidazole was higher (P smaller than 0.01) in strains classed as resistant to lymphoid leukosis. A similar difference (P smaller than 0.01) was observed with strains classed as resistant or susceptible to Marek's disease when the selections from one location were not included in the weighted average.

Animals↗
PubMed1975

Chromosome aberrations in cells infected with various strains of measles virus.

The incidence of chromosome fragmentation and/or breakage has been studied in cultured human embryonic diploid cells infected with different wild and vaccinal strains of measles virus. It seemed that for wild strains the mean incidence of fragmentation was two times higher, that of breakage two times lower than for the vaccinal strains.

Cell Line↗