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E D Stoufi

Publications and source records attributed to E D Stoufi.

7 recordsLinked to original sources
PubMed1987

Phenotypic analyses of mononuclear cells recovered from healthy and diseased human periodontal tissues.

Mononuclear cells were recovered from the gingival tissues of normal individuals and from patients with periodontal disease. Lymphocyte phenotypic markers were identified by immunofluorescence after reaction with monoclonal antibodies to T-cell subset markers. The normal tissues exhibited T4/T8 ratios almost identical to those in the peripheral blood. The diseased tissue cell ratios were significantly reduced, in both the adult periodontitis and the juvenile periodontitis groups (P less than 0.01 and P less than 0.02, respectively), indicating alterations in the T-cell subset distribution in these tissues. Each diseased patient showed a much decreased T4/T8 ratio in the gingival lymphocytes when these were compared with the peripheral blood ratio from the same patient. The T4/T8 ratios of the more severe sites were significantly lower than those of the less severe sites in the same disease category. The decreases in subset ratios could be attributed to statistically significant reductions in T4+-lymphocyte recoveries relative to peripheral blood and also to slight relative increases in T8+ lymphocytes. A highly significant (P less than 0.001) correlation between the average probeable periodontal pocket depth and the T4/T8 ratio of each disease category was demonstrated. The relative recoveries of B cells from the various tissues did not differ between diseased and normal tissues. It is suggested that T-cell regulatory expression in gingival tissues is distinct from peripheral blood regulatory expression and that there is a local immunoregulatory imbalance in periodontal disease.

Adolescent↗
PubMed1986

Significance of the head and neck in late infection in renal transplant recipients.

The present study was undertaken to evaluate the overall significance of the mouth and contiguous structures as sites of late opportunistic infection in renal transplant recipients, to define the flora of such infections, and to determine factors that place patients at risk of infection. Of 323 patients who underwent renal transplants, 57% developed infection at least 1 month postoperatively. Sex, donor source, or age did not influence the risk of infection. Of the posttransplant infections, 30.6% occurred in the head and neck, 21.9% in the respiratory tract, 23.7% in the urinary tract, and 10% at sites of trauma. Of head and neck infections, 16.4% were bacterial, 20.5% were viral, and 21.9% were fungal. In the remainder a definitive causative organism could not be identified. These results emphasize that the head and neck area is a major site of late opportunistic infection in renal transplant recipients.

Adult↗
PubMedDate not supplied

Diminished immunoglobulin synthesis after stimulation of mononuclear cells from periodontal disease tissue.

Gingival mononuclear cells from patients with adult periodontitis were cultured to determine the potential for IgG production. All samples (N = 27) showed IgG synthesis. Some samples demonstrated IgG synthetic activity over the entire period in culture, often with maximum synthesis after 8 days. Other samples showed IgG synthesis during the first half of the culture period and then little detectable production for the remainder. Cells were either untreated or treated with one of several different known mitogens during the culture period. Total IgG synthesis by peripheral blood lymphocytes was enhanced in the presence of pokeweed mitogen, E. coli lipopolysaccharide and killed. A. actinomycetemcomitans. In contrast, IgG synthesis by gingival cells in the presence of these same additives was significantly reduced when compared to gingival cell synthesis in the absence of mitogens; suggesting the presence of suppression in this system. These differences in responsiveness may be attributable to the unique combination of T cells found in the gingival tissues of patients with periodontal diseases. The patterns of IgG synthesis by gingival cells were different from those of peripheral blood cells from the same patient. This finding verified the distinctiveness of local immunoregulatory mechanisms in periodontal disease tissue from those found systemically.

Adult↗