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E Day

Publications and source records attributed to E Day.

At least 19 recordsLinked to original sources
PubMed1999

Usefulness of molecular detection of human herpesvirus-8 in the diagnosis of Kaposi sarcoma by fine-needle aspiration.

Kaposi sarcoma (KS), a multifocal angioproliferative disorder, occurs most commonly in patients with AIDS, in whom it remains an important cause of morbidity and mortality. KS is often in the differential diagnosis in HIV-infected patients undergoing fine-needle aspiration (FNA). FNA diagnosis of KS is usually made by morphologic observation of scant tissue fragments composed of bland spindle cells and crush artifact. Human herpesvirus-8 (HHV-8) has been identified by polymerase chain reaction (PCR) amplification of DNA samples isolated from various epidemiologic forms of KS. In an attempt to improve the accuracy of KS diagnosis by FNA, we analyzed for the presence of HHV-8 DNA in 13 spindle-cell lesions evaluated by FNA: KS, 8 cases; granulomatous inflammation due to Mycobacterium avium-intracellulare, 1; nodular fasciitis, 1; dermatofibrosarcoma protuberans, 1; dermatofibroma, 1; benign spindle-cell lesion of nerve sheath origin, 1; and 2 lesions with lymphoid hyperplasia. DNA isolated from archival Wright-Giemsa-stained glass slides was used for the PCR amplification of the HHV-8 DNA sequences. All of the cases diagnosed as KS and 1 of the lymphoid hyperplasia cases were PCR-positive for HHV-8 DNA, while all other cases of spindle-cell lesions were negative. The molecular demonstration of HHV-8 DNA may be a useful adjunct in the diagnosis of KS by FNA.

Adult↗
PubMed1998

Culicoides variipennis (Diptera: Ceratopogonidae) complex in Virginia.

Immature Culicoides variipennis (Coquillett) were sampled from aquatic habitats throughout Virginia, reared to adults, and examined by isozyme electrophoresis to assess their taxonomic status. Data from 22 counties showed that C. v. variipennis is widespread and common, the predominant taxon throughout Virginia, and genetically similar to C. v. variipennis in Maryland. Because C. v. variipennis is considered an inefficient vector of the bluetongue viruses, this observation is consistent with the low seroprevalence of bluetongue in indigenous livestock of the mid-Atlantic region. Culicoides v. sonorensis Wirth & Jones, considered to be the primary North American vector of the bluetongue viruses, was recovered in large numbers only from a wastewater lagoon at a dairy in southeastern Virginia, but also was detected at low levels in 6 other counties. Comparison of genetic distances and patterns of discriminating alleles among Virginia populations of C. v. variipennis and C. v. sonorensis showed that respective subspecies are genetically distinct and show no evidence of introgression, irrespective of geographic- and habitat-level sympatry. The persistence of a pure C. v. sonorensis population in a dairy wastewater lagoon may reflect physico-chemical factors that influence the distribution of immature C. variipennis complex populations. A better understanding of the distribution of the C. variipennis complex will benefit regionalization of U.S. exports of livestock and livestock germplasm to bluetongue-free countries.

Animals↗
PubMed1995

An alternately spliced mRNA encoding functional domains of murine MAdCAM-1.

cDNA clones representing a small (0.8 kb) form of murine mucosal addressin cell adhesion molecule-1 (MAdCAM-1) mRNA were obtained and sequenced. The sequence was identical to the published 1.6 kb murine MAdCAM-1 cDNA sequence, except that 432 nucleotides encoding the mucin-like and IgA-homologous portions were deleted. This cDNA most likely represents an alternately spliced mRNA. Substantial amounts of both the short and long MAdCAM-1 mRNAs are present in murine mesenteric lymph node. Ig fusion proteins displaying either the short or long forms of MAdCAM-1 can bind Mn(++)-activated JY cells bearing human alpha 4 beta 7 integrin, indicating that the two N-terminal Ig-like domains of MAdCAM-1 are sufficient to bind its integrin counter-receptor.

Alternative Splicing↗
PubMed1990

Generation of hybrid genes and proteins by vaccinia virus-mediated recombination: application to human immunodeficiency virus type 1 env.

The ability of poxviruses to undergo intramolecular recombination within tandemly arranged homologous sequences can be used to generate chimeric genes and proteins. Genes containing regions of nucleotide homology will recombine to yield a single sequence composed of portions of both original genes. A recombinant virus containing two genes with a number of conserved regions will yield a population of recombinant viruses containing a spectrum of hybrid sequences derived by recombination between the original genes. This scheme has been used to generate hybrid human immunodeficiency virus type 1 env genes. Recombinant vaccinia viruses that contain two divergent env genes in tandem array have been constructed. In the absence of selective pressure to maintain both genes, recombination between conserved homologous regions in these genes generated a wide range of progeny, each of which expressed a novel variant polypeptide encoded by the newly created hybrid env gene. Poxvirus-mediated recombination may be applied to map type-specific epitopes, to create novel pharmaceuticals such as hybrid interferons, to study receptor-binding or enzyme substrate specificities, or to mimic the antigenic diversity found in numerous pathogens.

Animals↗
PubMed1981

Is the periodic health examination worthwhile?

Periodic health examinations is a primary tool in the practice of preventive medicine. It has been identified as such and promoted for health maintenance and disease control since the early 1900s. And yet, it has never been proved cost-effective. In fact, recently is has become fashionable for certain physicians to challenge the professional time and public money spent on examinations of asymptomatic adults as nonproductive and wasteful, urging medical care only in the presence of complaints suggesting illness. A balanced assessment of the value of periodic health examinations requires definition of the content and method of the examination, the individuals to be examined, and the frequency with which the examination is performed. Some procedures have no or very little yield under any circumstances. Most procedures will be more effective for certain groups of examinees, particularly with reference to age and family and environmental factors. During the past decade, methods for health hazard or risk factor analysis have been developed which enable design of more efficient periodic health examination programming, targetting those population subgroups most apt to be harboring presymptomatic disease. It can be anticipated that refinement of risk-related examination methods in the next decade will firmly establish the value of periodic health examination as a basic tool in medical practice.

Adult↗
PubMed1971

Familial aggregation of a "new" connective-tissue disorder: a nosologic problem.

Three brothers are described who had a severe connective-tissue disorder and died in infancy. They were the offspring of a consanguineous union and members of a kindred in which other paternal relatives were found to have the benign hypermobile form of Ehlers-Danios syndrome. The findings in the three brothers were not clearly representative of any known connective-tissue disorder. The authors feel that the brothers' phenotype is best explained as a result of genetic interaction between the Ehlers-Danios gene and another single unspecified gene in double dose.

Abnormalities, Multiple↗
PubMed1969

Cell kinetic studies in mixed leukocyte cultures: an in vitro model of homograft reactivity.

The recognition phase of homograft immunity can be studied in vitro in the mixed leukocyte culture reaction. In this reaction, at the end of seven days, up to 30 per cent of the lymphocytes in culture "respond" to a single allogeneic cell stimulus. This paper presents evidence that the lymphocytes responding in culture divide with the generation time of 18-21 hours, with some asynchrony, and that a large percentage of the cells found at the end of the culture period may be the products by division of a small number of cells initially responding. Two estimates are made of the frequency of the initially responding unit (the cell(s) responsible for initiating the response). These are probably minimum estimates and are in the range of 1 in 200 to 1 in 2000 cells which can initially respond. This very high frequency of responding units as compared with the responding units in immediate type hypersensitivity (antibody production) is discussed.

Autoradiography↗
PubMed1968

Auscultation of foetal heart rate: an assessment of its error and significance.

AUSCULTATION OF FOETAL HEART RATE WAS SHOWN TO BE SUBJECT TO THREE TYPES OF ERROR: a random error, an error biased towards normality when the heart rate is fast or slow, and an error based on the inability to count the heart rate during contractions. In spite of these limitations a clinically observed foetal heart rate of more than 160 beats per minute was shown to be associated with significantly lower Apgar scores at birth. In contrast, a steady foetal heart rate of 100-120 beats per minute was not.

Electrocardiography↗