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Biomedical subjects

E Decuypere

Publications and source records attributed to E Decuypere.

At least 19 recordsLinked to original sources
PubMed2006

Non-ventilation during early incubation in combination with dexamethasone administration during late incubation: 1. Effects on physiological hormone levels, incubation duration and hatching events.

This study investigated the effect of non-ventilation of the incubator during the first 10 days of incubation and its combination with dexamethasone administration at day 16 or 18 of incubation on hatching parameters and embryo and post-hatch chick juvenile physiology. A total of 2400 hatching eggs produced by Cobb broiler breeders were used for the study. Blood samples were collected at day 18 of incubation, at internal pipping stage (IP), at the end of hatch (day-old chick) and at 7-day-post-hatch for T(3), T(4) and corticosterone levels determination. From 448 to 506 h of incubation, the eggs were checked individually in the hatcher every 2h for pipping and hatching. The results indicate that non-ventilation during the first 10-day shortened incubation duration up to IP, external pipping (EP) and hatch, had no effect on hatchability and led to higher T(3) levels at IP but lower corticosterone levels at 7-day-post-hatch. The injection of dexamethasone at days 16 and 18 of incubation affected hatching and blood parameters in both the ventilated and non-ventilated embryos differentially and the effect was dependent on the age of the embryo. Dexamethasone increased T(3) levels and T(3)/T(4) ratios but the effect was greater with early non-ventilation of eggs. Dexamethasone decreased hatchability but the effect was greater when injected at day 16 and especially in ventilated embryos. The effects of incubation protocols and dexamethasone treatments during incubation were still apparent in the hatched chicks until 7 days of age. The changes in T(3), T(4) and corticosterone levels observed in response to the early incubation conditions and late dexamethasone treatments in this study suggest that incubator ventilation or non-ventilation may influence the hypothalamic-pituitary-adrenal axis (HPA) regulation of stress levels (in terms of plasma corticosterone levels) and thyroid function in the embryo with impact on incubation duration, hatching events and early post-hatch life of the chick. Our results also suggest that some stages of development are more sensitive to dexamethasone administration as effects can be influenced by early incubation protocols.

Animals↗
PubMed2006

Impaired development of broiler chickens by stress mimicked by corticosterone exposure.

The effects of corticosterone (CORT) administration on the development of muscular tissues of broiler chickens (Gallus gallus domesticus) fed with diets differing in lipid content were investigated. The experimental chickens were given one of two experimental diets: high lipid diet (9.9% crude fat) or control diet, from 21 d of age. At 28 d of age, half of the chickens in each dietary treatment were exposed to CORT treatment, supplemented with 30 mg CORT/kg diet for 12 days, while the other half continued to consume the former diet. The zootechnical parameters were recorded at 21, 28, 35 and 39 d, and a blood sample was obtained from 8 birds of each group, respectively. The growth performance of broiler chickens was significantly depressed by CORT administration, but not by dietary treatment. Corticosterone treatment resulted in enhanced energy expenditure. The results indicate that the development of breast muscle was more susceptible to stress mimicked by CORT administration. The results suggest that corticosterone administration enhanced hepatic fatty acid synthesis and resulted in the redistribution of energy to abdominal store from peripheral tissues. Diet rich in lipid content was favorable to the central fat deposit in stressed broiler chickens.

Animals↗
PubMed2006

The relation between ultrasonographic observations in the oviduct and plasma progesterone, luteinizing hormone and estradiol during the egg laying cycle in ostriches.

In this study we investigated the temporal relationship between ovulation, egg formation, oviposition and the changes in plasma concentrations of progesterone, luteinizing hormone and estradiol-17beta during the egg laying cycle in farmed ostriches. In 10 egg-producing birds, transcutaneous ultrasound scanning was performed at 3h intervals and blood sampling at hourly intervals during a period of at least 48h (one egg laying cycle). In hens (n=8) that ovulated during the observational period, the ovulated egg was first detected 2h after oviposition; thus, ovulation occurred shortly after oviposition in all birds. During the period between two consecutive ovipositions, the developing egg remained for 9h in the proximal part (infundibulum, magnum or isthmus) and for 39h in the distal part of the oviduct (uterus). In ovulating hens, plasma progesterone concentrations showed a characteristic and consistent profile: from basal levels of around 0.1ng/ml concentrations started to increase 12h before oviposition, reached an average maximum of 3.5ng/ml at 3h before oviposition and returned to basal levels 3h and 30min after oviposition. Changes in plasma luteinizing hormone and estradiol-17beta concentrations showed comparable patterns of elevation and decline relative to the timing of oviposition and ovulation. However, variation in their individual basal concentrations was generally larger and peak values were less conspicuous than those of progesterone. In non-ovulating hens (n=2) neither progesterone, nor luteinizing hormone nor estradiol-17beta showed elevations to peak concentrations before oviposition. These data demonstrate that during the egg laying cycle of ostriches, events such as ovulation, egg development and oviposition evolve according to a rather strict time schedule, and that progesterone, luteinizing hormone and estradiol-17beta reach peak concentrations shortly before ovulation. Additionally, our findings also show that on-farm ultrasound scanning is a useful technique to discriminate between ovulating and non-ovulating hens.

Animals↗
PubMed2006

Influence of whole wheat inclusion on optimal feed withdrawal duration.

1. The objective of the present study was to examine whether the commonly applied duration of feed withdrawal (10 h) should be altered if broilers receive a commercial whole wheat (WW) diet instead of a completely pelleted (C) diet. 2. Inclusion of whole wheat in the diet did not affect weight gain, feed intake or feed conversion.3. The effect of different feed withdrawal (FW) durations on body weight loss was similar for both diets. 4. Gall bladder content was not significantly greater after longer feed withdrawal periods, irrespective of diet composition. Birds given the WW diet had significantly greater gall bladder contents than birds receiving the C diet. 5. Both diets had similar effects on wet weight of the total gut contents, mean relative wet contents of each gut region and percentage water content of the gut contents. 6. These results indicate that a feed withdrawal period of 10 h is sufficient for broilers on the WW diet.

Animal Feed↗
PubMed2006

Energy partitioning and thyroid hormone levels during Salmonella enteritidis infections in pullets with high or low residual feed intake.

This experiment was conducted to investigate whether feed efficiency, as measured by residual feed intake as a phenotypic trait, affects energy partitioning in pullets that have received Salmonella inoculation as an immune challenge. In each of 8 trials, energy partitioning was measured during 5 wk in 15-wk-old efficient (R-) and nonefficient (R+) pullets, which were housed per efficiency group in 2 identical climate respiration chambers. After 1 wk of adaptation, the pullets in 4 trials were orally inoculated with 10(8) cfu of Salmonella enteritidis; pullets in the remaining trials were not inoculated and served as controls. Heat production was calculated from continuous recordings of O(2) consumption and CO(2) production. Energy and N partitioning were recorded on a weekly basis. Blood samples for analyses on thyroid hormones were taken at 16, 17, and 19 wk of age. There were no interactions between efficiency type and Salmonella treatment or Salmonella treatment effects in energy partitioning, except for a short-term increase in heat production in inoculated pullets. Nonefficient pullets had higher gross energy and ME intake, higher estimated ME for maintenance, lower ME:gross energy ratio, and higher total heat production and nonactivity-related heat production compared with R- pullets. Triiodothyronine levels in R+ pullets were higher at 16 and 17 wk but were lower at 19 wk of age compared with R- pullets. Thyroxine levels were higher in R- at 16 wk and showed interactions between efficiency type and Salmonella treatment at 17 and 19 wk of age. Body weights and spleen weights did not differ between efficiency groups. Nonefficient pullets had higher heart, liver, and ovary weights and more large yellow follicles than R- pullets. There were no Salmonella effects on body and organ weights. We conclude that R+ pullets have a faster running energy metabolism and that they put more resources into organ development than R- pullets. Inoculation with Salmonella has a short-term effect on nonactivity-related heat production but does not affect energy partitioning, regardless of efficiency type.

Aging↗
PubMed2006

Consumers' preferences toward techniques for improving manual catching of poultry.

Growing interest in ameliorating animal welfare has prompted numerous studies that compare various aspects of manual and mechanical catching. In general, mechanical catching has been adopted as a realistic alternative for manual catching. The success of a catching machine as an alternative for manual catching does not only depend on its practical applicability, but also on its acceptance by "the general public." In the history of technological change, public perception of new technologies has often been ambivalent. Against this background, it is important to know how consumers perceive the production methods. This paper provides an evaluation of the preferences for catching methods by "society" to investigate whether there is a shift in preference due to the confrontation with video segments and the potential effect of awareness and importance attached to animal welfare on preference. Data were gathered through a questionnaire-based survey, including 450 respondents, performed in Belgium. For this study, the data indicated that when subjects were provided information concerning catching methods of broilers, they liked the technology much more. However, for those respondents without prior awareness of both catching methods or with high importance attached to animal welfare, giving information could not convince them of the advantage of using a mechanical catching machine. It is obvious that preference varies with the awareness and experience of the respondents. Future research should move forward from simple assessments of consumer concerns about the technologies and focus more directly on questions and issues related to the consumer's expected bottlenecks of these technologies. In this way, working at a better understanding can directly influence the acceptance of these technologies.

Adult↗
PubMed2006

Role of thyroid hormones, maternal antibodies, and antibody response in the susceptibility to colibacillosis of broiler genotypes.

The purpose of this study was to investigate whether differences in susceptibility to colibacillosis are associated with maternal antibodies, antibody response, and alterations in thyroid hormones [triiodothyronine (T3) and thyroxine (T4)] and to investigate the effect of genotype on the changes in T3 and T4 during challenge and antibody response. A challenge experiment was executed in 2 trials. Per trial, 24 chicks per genotype were challenged, and 20 chicks per genotype were controls. At 7 d of age, challenged chicks were intratracheally inoculated with 0.3 mL of Escherichia coli O78K80 and controls with 0.3 mL of PBS. All chicks were euthanized at 14 or 15 d. Thyroid hormone plasma concentrations and E. coli-specific antibody titers (AB) were measured at 7 d (T(3 d7), T(4 d7), and AB(d7)) and 14 or 15 d (change from 7 to 14 or 15 d was analyzed: DeltaT(3), DeltaT(4), and DeltaAB). Susceptibility was defined based on mortality, lesions, growth retardation, and eating behavior. There was a significant effect of challenge on T(3 d7); probably due to eating pattern in association with circadian rhythm. The challenge group was suggested to have functional hypothyroidism relative to the control group, indicating metabolic changes due to the challenge, and it was indicated that an antibody response was elicited. Differences in susceptibility were not significantly related to differences in T(3 d7), T(4 d7), DeltaT(3), or DeltaT(4) or to maternal antibodies (AB(d7)), but the antibody response tended to increase (decreasing DeltaAB) with increasing susceptibility. There were indications of genetic variation in T(4 d7), DeltaT(4), AB(d7), and DeltaAB, but there was no observed effect of genotype on DeltaT(3) and DeltaT(4) during challenge or on the antibody response. Further, there were indications that selection for growth traits has resulted in alterations in DeltaT(4) due to challenge, as indicated by a lower DeltaT(4) in the challenge group relative to the control group for more intensively selected genotypes as opposed to a higher DeltaT(4) for less intensively selected genotypes.

Animals↗
PubMed2006

Diet-induced thermogenesis and glucose oxidation in broiler chickens: influence of genotype and diet composition.

The main objectives of this study were to explore the role of diet-induced thermogenesis in the regulation of voluntary feed intake and to determine the glucose oxidation of broiler chicken strains, known to differ in glucose-insulin balance. From 2 to 7 wk of age, male broiler chickens of a fat and a lean line were reared on 1 of 2 isoenergetic diets with constant gross energy and carbohydrate levels but with substitutions between fat and protein. The low protein (LP/HF) diet contained 126 g of protein/kg and 106 g of fat/kg, whereas the low fat (LF/HP) diet contained 242 g of protein/kg and 43 g of fat/kg. There was no significant effect of the genetic background of the broilers on the glucose oxidation rate (as measured by stable isotope breath test) or protein oxidation (as measured by plasma uric acid levels). Considering the difference in carcass composition (fat content) of both lines, this leads to the hypothesis that the lines differ predominantly in fat metabolism. Although there was no line effect on plasma triglyceride and free fatty acid concentrations, it was hypothesized that there might be differences in fat oxidation or de novo lipogenesis, or both, between the genotypes. Diet-induced thermogenesis per metabolic body weight (kg of BW0.75) per 24 h, expressed per gram of feed intake, was not significantly influenced by genetic background or by diet composition. Therefore, a model linking feed intake to diet-induced thermogenesis, as postulated for adult mammals, could not be corroborated for growing broiler chickens.

Adipose Tissue↗
PubMed2006

Effects of genotype and feed allowance on plasma luteinizing hormones, follicle-stimulating hormones, progesterone, estradiol levels, follicle differentiation, and egg production rates of broiler breeder hens.

The aim of this study was to compare and relate plasma hormone levels of luteinizing hormone (LH), follicle-stimulating hormone (FSH), progesterone (P4), estradiol (E2), and the in vitro P4 production capacity of the largest yellow (F1) follicle granulosa cells with the laying performance of 2 genotypes (a standard S line and a dwarf cross-experimental E line) maintained under ad libitum (SA, EA) or restricted (SR, ER) feeding regimens. Age-related hormone changes were determined from 4 to 50 wk, hormone changes during the ovulatory cycle were determined during lay, and changes in follicle granulosa cell P4-producing capacity in response to LH with or without growth factors were measured in vitro at different ages. The mean laying rate was similar for SR, EA, and ER but were lower for the SA. Plasma LH and FSH concentrations increased with age in all groups and peaked at puberty. Restricted feeding delayed puberty in both genotypes. Concentrations of E2 and P4 increased after puberty in all groups but delayed in restricted hens. Plasma levels of LH, FSH, P4, and E2 before and after puberty were not correlated with egg-laying performance, but peak E2 levels were. Luteinizing hormone and P4 concentrations during the ovulatory cycle showed differences that may be associated with the different laying performances of the 2 genotypes under ad libitum and restricted feeding. The increase in plasma LH concentration (from basal) during the preovulatory surge was higher in the SR than in the SA but was similar for EA, ER, and SA. The increase in P4 was also higher in SR than in the SA with no difference between EA and ER. In vitro P4 production by granulosa cells in response to LH with insulin-like growth factors, and bone morphogenetic protein-7 was different among the SA, SR, EA, and ER; the EA, SR, and ER had greater responses, and the SA had less response. The presence of insulin-like growth factors and bone morphogenetic protein-7 enhanced LH effects depending on the feeding regimen and age of hen. This finding suggests that differences in laying performances among genotypes fed at different nutritional levels may be partly due to differences in processes associated with follicular maturation modulated by gonadotropins and growth factors. It is concluded that the age at puberty is determined mainly by feed allowance, irrespective of genotype, and that differences in laying performance may be due to a combination of factors that include changes in the levels of gonadotropins or ovarian hormones and growth factors, BW, and the condition of the different genotypes under different feeding allowances.

Aging↗
PubMed2006

Pathological features in dead on arrival broilers with special reference to heart disorders.

A gross postmortem investigation was done on 302 broilers that died between catching and slaughter to establish predisposing factors for dying in this period. Special attention was paid to heart disorders, which were established by determining the ratio of the right ventricle mass to the total ventricle mass (RV:TV) and to postmortem changes in hearts and lungs of broilers that were dead on arrival (DOA). Macroscopic pathologic lesions were found in 89.4% of DOA broilers. Signs of infectious diseases appeared to be most frequent (64.9%), followed by heart and circulation disorders (42.4%), and trauma (29.5%). The RV:TV was significantly higher for DOA broilers in comparison with slaughtered broilers. The prevalence of hearts with an abnormal RV:TV in DOA broilers was 34.4 vs. 4.1% in slaughtered broilers. The DOA broilers with an abnormal heart ratio more frequently showed ascites and hydropericardium. Postmortem changes in lungs depend on the position of the carcass the first several hours after death. Broilers, which remain in dorsal recumbency for several hours after death, develop engorged lungs. A good health status as well as more attention for the catching and crating process is crucial in decreasing the percentage of DOA broilers. Prevention of an increased heart ratio and of ascites will improve the livability in the broiler house and also decrease the DOA rate enormously.

Abattoirs↗
PubMed2006

Semiautonomous development of the extraembryonic membranes in the chicken embryo.

Based on an old paradigm that the extra-embryonic membranes develop semiautonomously from the embryo, it can also be postulated that subembryonic fluid (SEF) will be formed semiautonomously against embryonic growth, because the formation of SEF is mediated by the yolk sac membrane. In this study, we interfered in the development of SEF or the embryo. The acoustic resonance technique (which measures the resonant frequency of an excited egg) was used as a nondestructive tool to monitor the development of SEF. In the first experiment, in which the embryo was killed chemically with NaN3, it was proven that the formation of SEF continued, even when the embryo was killed after the initiation of the growth of the yolk sac membrane. In the second experiment, in which the development of SEF was inhibited chemically with amiloride, it was shown that the embryo developed further, although SEF formation was inhibited. In the last experiment, it was shown that the age of the flock affected the development of the embryo and the sudden decrease of the resonant frequency in a different way. However, some presetting conditions, such as storage, may affect both in a similar way. Our results further strengthen the idea that the formation of SEF develops semiautonomously against embryonic development by using the nondestructive acoustic resonance technique as an indirect method to monitor yolk sac membrane formation.

Aging↗
PubMed2006

Influences of feeding conventional and semisynthetic diets and transport of broilers on weight gain, digestive tract mass, and plasma hormone and metabolite concentrations.

Two replicate experiments were done to investigate if special diets provided to broilers in their last phase of life could reduce the negative effects of feed withdrawal and transport without an increased content of the digestive tract. In each experiment, 240 broilers were used. The experiments consisted of 2 interventions: the feed intervention and the transport intervention. The feed intervention took 72 h, in which broilers had full access to a conventional grower diet, a conventional grower diet with an increased carbohydrate level, a conventional grower diet with an increased fat level, a semisynthetic diet, or a semisynthetic diet with an increased carbohydrate level. The diets differed remarkably in carbohydrate content; carbohydrate content was approximately 67% in the semisynthetic diets and 42%, on average, in the conventional diets. Moreover, all of the carbohydrates in semisynthetic diets were highly soluble and digestible. The diets were compared with a feed withdrawal period of 24 h before transport. The transport intervention took 3 h; broilers were caught, crated, loaded, transported for 1.5 h, and had to wait in the crates for 1 h thereafter; or remained in the pens. After the transport intervention, blood samples were taken to determine plasma corticosterone, triiodothyronine, glucose, lactate, uric acid, nonesterified fatty acid, and triglyceride concentrations. Also, changes in live weight (LW) and digestive tract mass were assessed. The LW losses of broilers fed with semisynthetic diets after transport were 0.24% per hour less than of feed-withdrawn broilers. Moreover, intake of semisynthetic diets was approximately 200 g, whereas the intake of conventional diets was approximately 300 g. Therefore, the digestive tract mass as a percentage of LW was lower for semisynthetic-fed broilers in comparison with conventional-fed broilers, which can lead to a lesser degree of contamination during evisceration. No increase of corticosterone was found due to transport in semisynthetic-fed broilers. Semisynthetic feed with high carbohydrate concentration could be a good alternative for the feed withdrawal period held before transportation to the processing plant.

Animal Feed↗
PubMed2006

Monitoring of eggshell breakage and eggshell strength in different production chains of consumption eggs.

We first tried to monitor the critical points for eggshell breakage in different logistic chains. Second, we examined whether there was a difference in eggshell strength among eggs produced in different housing systems. Finally, we developed a model to investigate the relation between eggshell strength and the likelihood of an egg cracking during handling and grading. Four logistic chains with different housing systems (battery cages, furnished cages, aviary, and free-range), all housing Bovans Goldline chickens in their mid-lay (45 wk), were compared. In every chain, a randomized set of 1,500 eggs was sampled, and the strength was defined. At every critical point in every logistic chain, the eggs were reexamined for breakage. The classic and furnished cage systems showed the highest percentage of breakage directly at point of lay (6.73 and 10.72%), whereas the other systems showed lower breakage (1.94% in the aviary and 1.99% in the free-range system). Further, in the logistic chain, grading and packing of the eggs generated the second highest percentage of breakage (from 1.50 to 2.65%). Breakage due to transportation ranged from 0.16 to 2.65%. There was a significant difference among the eggshell strength (shell stiffness and damping ratio) of eggs from chickens in different housing systems, showing eggs from chickens in the aviary system to be stronger than cage eggs (classic and furnished) and free-range eggs to be weaker than the other eggs. A significant correlation was found between eggshell strength and the likelihood of breakage in the production chains. In conclusion, it was first shown that, besides the laying, packing of the eggs is a critical point in the logistic chain of consumption eggs; second, the strength of the eggs in the different housing systems differed, and, finally, the eggshell stiffness and damping ratio of consumption eggs are an acceptable measure for rapid eggshell quality assessment and could provide a good predictive value for eggshell breakage in all types of table egg production chains.

Animal Husbandry↗
PubMed2005

Peripheral ghrelin reduces food intake and respiratory quotient in chicken.

Ghrelin injection, either centrally or peripherally strongly stimulates feeding in human and rodents. In contrast, centrally injected ghrelin inhibits food intake in neonatal chickens. No information is available about the mechanism and its relationship with energy homeostasis in chicken. Since ghrelin is predominantly produced in the stomach, we investigated the effect of peripherally injected ghrelin (1 nmol/100g body weight) on food intake and energy expenditure as measured in respiratory cells by indirect calorimetry for 24h in one-week-old chickens. Plasma glucose, triglycerides, free fatty acids, total protein and T(3) were measured in a separate experiment until 60 min after injection. Food intake decreased until at least 1h after intravenous ghrelin administration. The respiratory quotient (RQ) in ghrelin-injected chickens was reduced until 14 h after administration whereas plasma glucose and triglycerides concentrations were not altered. Free fatty acids and total protein levels also remained unchanged. Ghrelin did not influence heat production and this was supported by the absence of changes in plasma T(3) levels when compared to the control values. In conclusion, peripheral ghrelin reduces food intake as well as RQ and might influence the type of substrate (macronutrient) that is used as metabolic fuel.

Animals↗
PubMed2005

Thyroid hormone availability and activity in avian species: a review.

The intracellular thyroid hormone (TH) availability is influenced by different metabolic pathways. Some of the changes in intracellular TH availability can be linked to changes in local deiodination and sulfation capacities. The secretion of the chicken thyroid consists predominantly of thyroxine (T4). TH receptors (TRs) preferentially bind 3,5,3'-triiodothyronine (T3). Therefore, the metabolism of T4 secreted by the thyroid gland in peripheral tissues, resulting in the production and degradation of receptor-active T3, plays a major role in thyroid function. Food restriction in growing chickens increases hepatic type III deiodinase (D3) levels but decreases growth hormone (GH)-dependent variables such as plasma insulin-like growth factor-I (IGF-I) and T3 concentrations. Refeeding restores hepatic D3 and plasma T3 to control levels within a few hours. It can be concluded that the tissue and time dependent regulation of the balance between TH activating and inactivating enzymes plays an essential role in the control of local T3 availability and hence in TH activity. Two separate genes encode multiple TR isoforms, i.e. TRalpha and TRbeta. These TRs consist of a DNA-binding domain, a ligand-binding domain, a hinge region and an amino-terminal (A/B) domain. TRs mediate their effects on transcription by binding as homodimers or heterodimers to the TH response elements (TREs). Also, unliganded TRs can bind to TREs and may so modulate transcription of target genes.

Animals↗
PubMed2005

Feed allowance-genotype interactions in broiler breeder hens.

Ad libitum feeding reduces livability and reproductive fitness in broiler breeder hens. Two genotypes, a standard (S) and an experimental dwarf broiler breeder (E), were fed ad libitum (SA and EA, respectively), restricted at 55% of ad libitum feed intake (intermediate restriction) from 6 to 15 wk of age (SI and EI, respectively), or restricted (SR and ER, respectively) to match a standard growth curve with a diluted mash feed (2,400 kcal/kg). The experiment was repeated at 2 locations (experiment 1 = 672 hens in pens from 0 to 40 wk; experiment 2 = 420 hens in pens and cages from 0 to 53 wk). Feed restriction reduced adult BW by 20% compared with ad libitum feeding, delayed sexual maturity by 2 to 4 wk, and improved livability. Hens fed the intermediate diet immediately compensated after 15 wk of age to reach BW, sexual maturity, and livability close to those of ad libitum-fed hens. The E genotype exhibited better tolerance to ad libitum feeding than the S genotype in all measured aspects. Average laying rate during the first 24 wk of lay was 66.4, 77.4, 69.9, 47.2, 57.9, and 72.4% for EA, EI, ER, SA, SI, and SR respectively in experiment 2. Egg abnormalities (double yolk, shell problems) decreased after the peak of lay but remained consistently higher for S compared with E, and for ad libitum and intermediate diets compared with the restricted diet. Yolk deposition rate was measured by a double dye technique. Duration of yolk rapid growth was 8.8 d in E and 9.3 d in S hens (P < 0.001), but this difference did not explain the observed variations in laying rate. The potential to increase feed allowances even with a diluted diet in broiler breeder hens requires adapted genotypes.

Aging↗
PubMed2005

Effects of turning duration during incubation on embryo growth, utilization of albumen, and stress regulation.

Eggs from Cobb broiler breeders were incubated for 18 d. Eggs were not turned (T0) or were turned until 9 (T9), 12 (T12), 15 (T15), or 18 (T18) d. First, the effects of turning on embryo and albumen weights were studied. Samples of eggs were opened at d 9, 12, 15, and 18 for embryo and albumen weighing. The results show that embryos from unturned eggs had lower weights and higher remaining albumen weights than those from turned eggs. At d 18, albumen utilization was completed in the T12, T15, and T18 groups only. Also, further turning until d 15 and 18 increased embryo weights. The responsiveness of the embryo after adrenocorticotropic hormone (ACTH) injection was studied to test stress control in embryos. Blood samples were collected from embryos at 60 and 150 min after injection at d 12, 15, and 18 and were analyzed for corticosterone concentrations. The results showed that basal corticosterone levels increased with embryo age. At 60 min after ACTH injection, corticosterone levels were lower at d 12 than at d 15 and 18. At 150 min after ACTH injection, corticosterone levels followed different trends according to incubation stage and turning duration. The highest basal corticosterone levels were obtained with the T15 group at d 15 and 18. Also at d 18, corticosterone levels in the T15 group were the highest at 150 min after ACTH injection. We concluded that egg turning was required during incubation until d 12, at least, and should not be stopped until after d 15.

Agriculture↗