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Biomedical subjects

E Deinlein

Publications and source records attributed to E Deinlein.

At least 19 recordsLinked to original sources
PubMed1992

[Patients with late-life vision damage in occupational rehabilitation. Preliminary ophthalmologic treatment, concomitant manifestations and an illustration provided by the growing number of patients with juvenile diabetes].

The rehabilitation centre in Veitshöchheim (near Würzburg) offers different kinds of training programs for the adult visually handicapped. This is usually preceded by a long time of unemployment. Diabetes mellitus was the third most common disease at the centre and 25% of the patients with diabetes mellitus were already legally blind. In a retrospective study we looked at the medical and ophthalmological treatment and the education the 76 patients with diabetes mellitus had received before coming to the rehabilitation center. All charts from February, 87, to September, 90, were included in the study. According to the classification of visual acuities by the WHO we formed four groups (less than or equal to 1/50; 1/35-0.1; 0.125-0.3; greater than 0.3) and compared them with each other. In the groups with the lowest visual acuity the medical management of the diabetes mellitus had been much poorer than in the better groups. Related to the bad visual acuity was the higher incidence of proliferative diabetic retinopathy and the significantly lower percentage of panretinal lasercoagulations. 47% of the patients with diabetes mellitus had been trained in jobs that would not have been recommended to a potential visually disabled by an ophthalmologist or the office for labour exchange. Only in 10% of the cases could a job be maintained by the help of technical devices (including low vision aids). To ensure best medical treatment and counselling concerning the choice of an appropriate job a close team work between practising ophthalmologist, general practitioner, and the office for labour exchange is needed. Thereby the cost and psychological impact of a retraining program could hopefully be lowered.

Adult↗
PubMed1988

Diagnostic significance of flow cytometric DNA analysis applied for the detection of cancer cells in bronchial washing fluid.

Since both DNA aneuploidy and increased proliferative activity are important characteristics of malignant neoplasms, flow cytometric (FCM) analysis was used to examine the cell content in bronchial washing samples obtained via fiberoptic bronchoscopy from 73 patients. The results were compared with the results of histology and conventional cytology. The patients included 30 with bronchial carcinomas, 12 with bronchopneumonia and 31 with no evidence of lung disease. Of the 30 patients with histologically confirmed lung cancers, 25 showed either aneuploid stem lines (19 cases) or high levels of proliferation (6 cases) as determined by analysis of cell-cycle stages. The same rate of cancer cell detection was obtained by cytology. In the 43 cases with neither histologic nor clinical evidence of malignancy, FCM data yielded 5 false-positive results, as compared to 4 erroneous suspicions of cancer by cytology. From these data, it is concluded that FCM measurements of both DNA ploidy and proliferative activity may complement conventional cytology in the recognition of bronchial carcinomas.

Bronchitis↗
PubMed1987

Morphological and flow-cytometric characterization of a trichilemmoid carcinoma cell line in vitro.

A cell line derived from a trichilemmoid carcinoma of a 85-year-old male was established in vitro and maintained in culture for 14 months. From the explant culture to the second passage, the tumour cells propagated slowly. After 6 months (third passage) faster growth behaviour was noticed. In culture the neoplastic cells continued synthesizing abundant glycogen. The DNA histogram of original neoplastic cells revealed a seemingly normal unimodal DNA distribution. The tumour DNA remained apparently stable during the subsequent months in culture. However, after 6 months in culture, flow cytometry disclosed a spontaneous shift in nuclear DNA content. This study enabled us to establish in vitro the malignant properties of the tumour and strongly suggested that it was derived from trichilemmal cells.

Aged↗
PubMed1987

Cell kinetics of the human anagen hair follicle. Flow cytometric studies in healthy and psoriatic subjects.

Anagen hair follicles obtained from both healthy (n = 7) and uninvolved psoriatic (n = 4) scalps were segmentally analyzed for proliferative activity using DNA flow cytometry. Hair follicle kinetics were almost equal in either group except for the infundibular portion which exhibited significant increase of S-phase values in psoriatic patients. Maximum proliferation was disclosed within the bulbar segment. This study confirms that cell kinetics behavior of hair follicles from uninvolved scalp of psoriatics compared with those from healthy scalps is altered in the infundibular portion only.

Adult↗
PubMed1986

[Cell kinetics of human scalp hair--impulse cytophotometric studies].

DNA Flow Cytometric measurements (DNA-FCM) were performed on both anagen hair bulbs of 21 healthy volunteers (11 females, 10 males; aged between 18 and 21 years) and tufts of plucked hairs from 43 test persons (17 showing normal trichograms, 12 with telogen and 14 with dystrophic hair root pattern; aged between 19 and 49), subsequently submitted to routine trichogram investigation. The high proliferative activity of anagen hair bulb cells was accurately registered by FCM. Neither sex nor hair color nor hair length were found to influence the proliferative parameters. The degree of hair loss-as diagnosed on account of the trichogram-was also reflected by the DNA histograms; telogen and dystrophic hair root patterns, however, could not be discerned by FCM.

Adolescent↗
PubMed1986

Comparative morphological and growth kinetics studies of human hair bulb papilla cells and root sheath fibroblasts in vitro.

Dermal papillae isolated from anagen hair bulbs obtained from biopsy specimens from five subjects with normal hair pattern, and fibroblasts derived from the mesenchymal root sheaths (RSF) of the same hair follicles were separately grown in culture and the cell-cycle distribution pattern on different days was analyzed by applying DNA flow cytometry (FCM). Papilla cells (PC) exhibited distinctive morphological features by forming cell aggregates differing from RSF with respect to cell shape and growth pattern. They also proliferated remarkably more slowly than RSF. DNA-FCM analysis showed that both PC and RSF demonstrated synchronous fluctuations in the percentage of cells in G1/0, S and G2 + M phases during the period of subculture.

Adult↗
PubMed1985

Clonal heterogeneity in a case of Merkel cell carcinoma demonstrated by flow cytometry.

A 66-year-old female patient is presented in whom a rapidly growing tumour developed on the glabella. Light and electron microscopy confirmed Merkel cell carcinoma. Flow cytometry (FCM), performed of a tumour specimen, yielded multiple aneuploid stem lines. The FCM data are discussed with regard to the histological features and the biological behaviour of the tumour.

Aged↗
PubMed1984

[Vital microscopy of nailfold capillaries in progressive scleroderma in relation to the clinical picture].

Capillary microscopic investigations of the nailfold were performed in 38 patients presenting Raynaud's phenomenon (systemic sclerosis: diffuse type n = 6; systemic sclerosis: CREST syndrome n = 21; Raynaud's disease n = 7; mixed connective tissue disease (MCTD) n = 4). A control group of 15 healthy persons was investigated for comparison. 3 stages of capillary alteration were established in patients with systemic sclerosis by recording number (per 2 mm microscopic field diameter), morphology, colour and size of nailfold capillaries. Additional criteria like pericapillary microhemorrhages and disordered capillary blood flow were considered as well. Significant correlations of capillary microscopie findings to different subsets of systemic sclerosis including MCTD could not be proven. Cases provisionally classified as Raynaud's disease could not be assigned to systemic sclerosis on account of capillary microscopy. A clear correlation was found, however, between the grade of capillary alteration and both severity of clinical features and duration of the disease. All cases with capillary changes of stage 3 showed clinical correlation to esophageal dysmotility , also proven in 7 out of 13 patients with stage 2 capillary alteration.

Adult↗
PubMed1983

DNA flow cytometric measurements in inflammatory and malignant human gastric lesions.

102 gastroscopically taken biopsy specimens which were normal (n = 28) or showed superficial gastritis (n = 18), chronic atrophic gastritis (n = 18), gastric ulcers (n = 19) and gastric carcinomas (n = 19) were submitted to FCM analysis. Carcinomatous specimens were readily recognized by either ploidy abnormality or significantly raised S- and G2 + M values. Intestinal-type and diffuse carcinomas could not be distinguished by proliferation kinetics, however, diffuse carcinomas showed a higher rate of aneuploidy. Chronic atrophic gastritis and gastric ulcers, though significantly differing from both normal tissue and superficial gastritis, exhibited similar proliferation characteristics. In gastric ulcers, an S-phase proportion of more than 12% was correlated with histological detection of cellular atypias and a proliferative tendency.

Adult↗
PubMed1983

Epithelial cell proliferation of oral lichen planus in patients treated with an aromatic retinoid.

In 4 patients with lichen planus mucosae (l.p.m.) biopsies were taken from the involved and uninvolved buccal sides before treatment and from the affected side after a 3-week systemic therapy with aromatic retinoid (Ro 10-9359; 50 mg/die). After in vitro labelling with 3H-thymidine the epithelial cell proliferation was determined histo-autoradiographically. In atrophic lesions of l.p.m., increased 3H-thymidine labelling indices were observed during retinoid therapy. After 3 weeks of treatment the augmented relative portion of labelled basal cells (quotient LIbas/LIsuprabas) was found to be lowered, indicating a tendency of proliferating cells to regain normal distribution in the progenitor compartment of the mucosa epithelium.

Adult↗
PubMed1983

[Immunopathologic studies in herpes gestationis].

In a 19-year-old Turkish primigravida with clinically and histologically confirmed herpes gestationis, immunohistological investigations revealed deposits of C3, C4, IgG, and IgM in the dermoepidermal junction. Furthermore, C3 and IgM were found in the upper dermal vessels, suggesting a concomitant vasculitis, which was also observed by capillary microscopy. ANA were present in the serum of the patient, a circulating factor capable of fixing complement to the basement-membrane (HG-factor) was demonstrable as well. Lymphocytotoxic antibodies, however, could not be detected. HLA-typing disclosed HLA-B8 haplotype.

Adult↗
PubMed1982

DNA-ploidy rates in oral leukoplakias determined by flow-cytometry.

In 11 patients with histologically confirmed benign (n = 4) precancerous (n = 7) oral leukoplakias, the nuclear DNA distribution patterns were analyzed by flow-cytometry. In two of three precancerous lesions exhibiting severe dysplasia, monoclonal hyperdiploid (aneuploid) cell populations were demonstrated. Aneuploidy was not observed in early or moderate dysplasias. To fully assess the diagnostic significance of flow cytometry in oral premalignancies, a larger quantity of specimens must be examined.

Aneuploidy↗
PubMed1981

Immune phenomena in patients with pustular bacterid.

In 20 patients with histologically confirmed pustular bacterid of immunological status was checked including lymphocyte and granulocyte components of the cellular immune system as well as HLA typing. For comparison the same investigations were carried out in 20 healthy controls. In contrast to the controls, in the group of patients the chemotactic activity of polymorphonuclear leukocytes as well as their ability to engulf either vital or heat-inactivated Candida albicans cells was found to be slightly impaired (p less than or equal to 0.01 and 0.05, respectively). Killing of C. albicans cells by polymorphonuclear leukocytes were strikingly impaired (p less than or equal to 0.001). NADPH-dependent oxidase activity, however, was found to be normal. Impairment of chemotaxis and killing activity could not be substantiated in AB pool serum, thus a serum-dependent disorder of granulocyte function has to be assumed. Lymphocyte response to phytohemagglutinin was reduced in all patients, whereas intracutaneous test reactivity to microbial antigens was normal. T and B cell counts in peripheral blood were within normal range. HLA typing revealed a significantly increased prevalence rate of HLA-B14 as compared with the control group and other patients.

Adult↗
PubMed1980

Disorder of cellular immunity in pemphigus vegetans.

In an untreated 26-year-old female patient suffering from Neumann's pemphigus vegetans for 8 months (with neither preceding thymoma nor myasthenia gravis), several in vivo and in vitro phenomena of immunodeficiency involving both T and B cell system were disclosed. A diagnosis of defective immune state was established on the basis of the low IgG-serum level (though pemphigus antibodies were present), reduced T cell count in blood, weak PHA stimulation of lymphocytes, lack of skin reactivity to recall antigens and to most other bacterial antigens tested. Impaired chemotaxis and a deficient intracellular killing of Candida albicans by PMNL were also found. The conclusion is drawn that a combined disorder of both the B and T cell system is involved in the etiology of pemphigus vegetans, even when thymoma is ruled out.

Adult↗