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Biomedical subjects

E Delain

Publications and source records attributed to E Delain.

At least 91 records · Page 5Linked to original sources
PubMed1969

Electron microscopy of the circular kinetoplastic DNA from Trypanosoma cruzi: occurrence of catenated forms.

Circular kinetoplastic DNA was fractionated from Trypanosoma cruzi DNA by Hg-Cs(2)SO(4) gradient. Covalantly closed circular DNA was isolated by buoyant density centrifugation in ethidium bromide CsCl density gradients. Electron microscope studies have shown that kinetoplastic DNA molecules are in the form of open and closed circles of contour length 0.45 mu, the smallest known microbial DNA. DNA molecules are also in the form of catenanes consisting of two or more topologically interlocked circular units of the monomer size 0.45 mu. Associations of numerous circles have also been described and confirmed by band sedimentation. Long linear DNA molecules were also seen, their size ranging between 2 and 12 mu. These molecules are free or are attached to associations of circles.

DNA↗
PubMed1969

Abnormal circular DNA molecules induced by ethidium bromide in the kinetoplast of Trypanosoma cruzi.

The trypanocidal drug ethidium bromide induced abnormal circular DNA molecules with high frequency in the kinetoplast of Trypanosoma cruzi. These molecules are circular dimers, trimers, or higher circular forms with a contour length which is a multiple of the monomer length usually found in the kinetoplastic DNA of normal trypanosomes. These abnormal molecules represent more than 30 per cent of the kinetoplastic DNA, while in normal kinetoplastic DNA we only observed three circular dimers out of several thousand molecules measured.

Bromides↗