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Biomedical subjects

E Delgado

Publications and source records attributed to E Delgado.

18 recordsLinked to original sources
PubMed1992

Isolation of an immunodiagnostic Taenia solium coproantigen.

Antigens of Taenia solium can be demonstrated by ELISA technique in stool samples of tapeworm carriers. In order to isolate these immunodiagnostic components from stool, fecal samples of known T. solium carriers were subject to chromatographic purification procedures: immunoaffinity chromatography and a subsequent two-step high performance liquid chromatography yielded a 60-kDa protein that was shown to react in a Western blot with polyclonal anti-T. solium IgG. The protein isolated appeared to be the major antigenic component excreted with the feces of parasite hosts.

Animals↗
PubMed1991

Detection of Taenia solium antigens in merthiolate-form preserved stool samples.

Cysticercosis can be controlled by mass treatment of Taenia solium carriers in hyperendemic areas. An automated screening method suitable for mass application is required to determine the prevalence of taeniasis and the efficacy of its treatment, since existing methods of carrier detection are considered insufficient for this purpose. Thus, a biotin/avidin-enhanced enzyme-linked immunosorbent assay (ELISA) was designed, which detected T. solium antigens in supernatants of merthiolate-formalin preserved stool samples. The assay distinguished the nine T. solium carriers tested from 41 negative controls, but cross-reactivity with Taenia saginata was noted.

Animals↗
PubMed1990

Recurrent dislocation of the elbow.

Twelve cases of recurrent dislocation of the elbow have been studied with special reference to the cause of instability, the operative findings and the results of treatment. We consider the major cause to be insufficiency of the posterolateral capsule and ligaments, and therefore the best treatment is a posterolateral capsulorraphy.

Adolescent↗
PubMed1990

Molecular biology of circulatory shock. Part II. Expression of four groups of hepatic genes is enhanced after resuscitation from cardiogenic shock.

Despite an initially successful resuscitation from circulatory shock, multiple organ failure (MOF) develops in some patients. The marked biochemical alterations associated with shock and MOF include clinically important changes in gene expression, such as altered rates of albumin and procoagulant synthesis. To characterize the MOF-associated changes at the cellular level, sequential liver biopsies were obtained from a swine model of cardiogenic shock associated with MOF. Preshock and postresuscitation biopsies were used not only to create a complementary DNA (cDNA) library but also to screen, to confirm, and, in nine out of 12 cases, to specifically identify genes whose expression is enhanced at least fivefold after resuscitation. The twelve genes thus characterized can be separated according to function into distinct groups, including the acute-phase genes and the heat-shock genes. Expression of acute-phase genes is liver specific and is essential for systemic homeostasis; heat-shock gene expression is generic to all cells and important for intracellular homeostasis.

Acute-Phase Proteins↗
PubMed1990

[Evaluation of the degree of compliance in arterial hypertension protocol].

We report an evaluation of the hypertension protocol. Its aims were to assess the degree of compliance with its variables, the number, age and sex of hypertensives, and the rate in whom blood pressure levels were controlled in the patients included and not included in the protocol. The study was carried out in the Basauri Teaching Unit from January 1987 to June 1989. We reviewed all the parameters of the medical and nursing protocol of 1,429 patients diagnosed as hypertensive and the mean value of the three last blood pressure readings. A total of 67.2% of patients were included in the protocol. The compliance was higher than 80% in most of its items; 61.8% of the prescribed nursing controls were carried out. Blood pressure was less than or equal to 159/94 in 68.1% of patients. This rate was higher in the patients included in the protocol. The protocol was useful for the control of hypertension, although it should be modified directing it to primary care.

Adult↗
PubMed1987

Osteochondroma induced by reflection of the perichondrial ring in young rat radii.

In order to investigate the effects of reflection of the perichondrial ring in osteochondroma formation, the perichondrial rings of the epiphyseal growth plates in 42 young rat radii were turned to the metaphyseal periosteum by means of blunt dissection. Seven days after surgery a small nest of chondrocytes appeared on the metaphyseal-diaphyseal bone surface at the level of the tip of the reflected perichondrial ring. From the 9th to the 15th days the histological pattern of the osteochondroma was established. The osteochondroma was not connected with the hypertrophic cartilage of the growth plate. During the third and fourth weeks the osteochondroma began to regress with the disappearance of the cartilage nest. During the development of the lesion the bone grew normally and the growth plate migrated distally while the lesion remained at its initial site. The growth plate zone devoid of perichondrial ring was covered by fibrous connective tissue and no removal of the perichondrial ring occurred. These results suggest that the origin of this osteochondroma is the perichondrial ring cells whose polarity has been surgically changed.

Animals↗
PubMed1985

Changes in young rat radius following excision of the perichondrial ring.

The distal growth plate of the radius was exposed in young rats and the perichondrial ring and fibrous covers were removed at the exposed surface. In addition, a small portion of either the adjacent epiphysis or of the adjacent metaphysis was removed also. In the following days, important alterations in bone structure were observed at the level of the removed perichondrial ring. The most relevant changes were an enlargement of the growth plate at the exposed surface that grew in an abnormal direction, proliferation of bone trabeculae at the level of the excised perichondrial ring, and bending of the bone. No regeneration of the perichondrial ring occurred. These changes support both the role of the perichondrial ring in the mechanical constraint of the growth plate, and the induction of bone formation by the hypertrophic cartilage at the level of the absent perichondrial ring.

Animals↗
PubMed1985

Multivacuolated cells in human cartilage canals.

Light and electron microscopic examination of cartilage canals in 39 infants (from fetuses to 2 years of age) revealed the presence of multivacuolated cells in the connective tissue of the canals. These cells showed an irregular and electron-dense nucleus and cytoplasm; the latter was almost filled by large, irregular, membrane-bound vacuoles without or with hardly apparent content. Histochemistry revealed a weakly positive stain with periodic-acid-Schiff, oil-red-o and muramidase techniques in these cells which seem to be degenerated macrophages.

Bone and Bones↗
PubMed1985

Antibodies to Bartonella bacilliformis as determined by fluorescence antibody test, indirect haemagglutination and ELISA.

Two strains of Bartonella bacilliformis were cultured on Columbia agar supplemented with 5% defibrinated human blood. Antigens prepared from these cultures were used for determination of antibodies by fluorescence antibody test (FAT) indirect haemagglutination (IHA) and an enzyme immunoassay (ELISA). One hundred and eighty-seven human sera from B. bacilliformis-endemic areas of Peru were tested of which 63.6% were reactive. ELISA was the most sensitive test, followed by FAT and IHA. IgM antibody was determined by FAT with the IgM fraction of test sera. It was present not only in patients with Oroya fever but also in some healthy individuals as well as in one patient with chronic bartonellosis (verruga peruana). Substantial differences in antigenic activity between the two strains of B. bacilliformis were not observed. Cross reactivity with sera containing antibodies to bacteria other than B. bacilliformis was not noted. For identification of Oroya fever patients in the field, an eosin/thiazine stain of blood smears was found to be appropriate.

Antibodies, Bacterial↗
PubMed1985

Human fascioliasis in Cajamarca/Peru. I. Diagnostic methods and treatment with praziquantel.

In a prospective study 34 Peruvian fascioliasis patients were followed up for three months after treatment with praziquantel. The rapid sedimentation of faeces was demonstrated to be the most appropriate parasitological technique for the diagnosis of Fasciola hepatica infections in man compared to the MIF concentration or the examination of duodenal fluid by Enterotest. After therapy with different doses of praziquantel only 21% of the patients stopped to excrete worm eggs although 17 patients had been treated repeatedly. During the period of observation all patients presented a decreasing number of symptoms. It is concluded that praziquantel is not very active against F. hepatica in man.

Adolescent↗
PubMed1985

Radiation-induced osteochondroma-like lesion in young rat radius.

To investigate the effects of radiation on the perichondrial groove of Ranvier in osteochondroma development, the external surface of the distal growth plate of the radius in both forelimbs of 30 ten-day-old rats was exposed to a single low dose of radiation (150 r), which was focused on the perichondrial groove. This induced the formation of a chondrocyte nest at the proximal external edge of the growth plate (five to nine days after irradiation). With advancing longitudinal growth of the bone, the chondrocyte nest occupied a diaphyseal position. At nine to 11 days the chondrocyte nest underwent endochondral ossification. At 13-15 days, this osteochondroma-like lesion began to regress with the disappearance of the chondrocyte nest. After 19-21 days, only an irregularly thickened cortical bone remains at the osteochondroma site. Although the possible role of the growth plate subjacent to the irradiated perichondrial groove must be taken into account, the continuity between the perichondrial groove and the osteochondroma, which is separated from the growth plate by the periosteal ring (bone bark), suggests that the perichondrial groove was involved in osteochondroma-like lesion development.

Animals↗
PubMed1985

Immunodiagnosis of cysticercosis: standardization of ELISA and its application to field conditions.

A standardization procedure for the enzyme-linked immunosorbent assay (ELISA) is described. Results were expressed as antibody units (ABU) which represent a multiple of absorbance of the negative control. The method was applied to immunodiagnosis of cysticercosis by IgG antibody determination. Different incubation temperatures had no substantial effect on results. There was a fair correlation between results obtained under field conditions in Cajamarca/Peru and results obtained under optimal laboratory conditions in Hamburg/FRG. Activity of antigen that had been lyophilized and stored under vacuum at ambient temperature was similar to the activity of antigen that had been stored frozen. There was a considerable loss of activity when antigen had been adsorbed to microtitre plates which were then stored in evacuated plastic bags at room temperature. Reproducible results were obtained both under optimal laboratory conditions and in the field.

Adsorption↗
PubMed1979

Rate-dependent action potential changes in rat atrium.

Experiments in isolated left atria from rat hearts were performed in order to study the effects of stimulation rate on the transmembrane action potential. 1. Two components (fast and slow) of the action potential upstroke could be differentiated by adding MnCl2 to the perfusion solution. 2. With the increase in rate of stimulation over the control cycle length (500 msec), amplitude, Vmax and action potential duration at 80% of repolarization (D80) diminished in normal Krebs. In Mn-containing Krebs, only a slight reduction in amplitude was recorded. Resting potential and action potential duration at 20% (D20) and 50% (D50) of repolarization were only slightly affected in normal Krebs and not at all in Mn-Krebs. 3. Low rates of stimulation in normal Krebs increased D50 only slightly; however, D80 increased significantly while other parameters remained constant. No effects were seen in Mn-Krebs. 4. The results with Mn-Krebs indicate the importance of slow inward current on changes induced by stimulation rates. A possible mechanism relating intracellular calcium concentration and the outward K current, depending on the rate of stimulation, is discussed.

Animals↗