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E Deveaux

Publications and source records attributed to E Deveaux.

8 recordsLinked to original sources

Bacterial microleakage of Cavit, IRM, and TERM.

In this in vitro study, a model system was developed and tested to evaluate the sealing ability of temporary restorative materials used in endodontic access preparations. The materials studied, Cavit, IRM, and TERM, were tested on 40 premolars against a known bacterial species, Streptococcus sanguis. The leakage of bacterial cells was checked 4 and 8 days after initial immersion in the culture. Thermocycling was introduced on the fourth day. After 8 days the cement thicknesses were measured after the teeth had been longitudinally sectioned. Before and after thermocycling, IRM was less leakproof than Cavit (p < 0.05) and TERM (p < 0.05). Thermocycling aggravated percolation in the case of IRM, and decreased the tightness of Cavit, whereas TERM remained leakproof. The thicknesses were as follows: Cavit, 3.73 mm; IRM, 3.45 mm; and TERM, 5.49 mm. There was no statistically significant relationship between thickness and tightness.

Analysis of Variance

Survival of Prevotella intermedia (Bacteroides intermedius) in transport media.

Five transport media were selected for testing, in vitro, the survival of a pure strain of Prevotella intermedia (Bacteroides intermedius) for 6, 24, and 72 hours. Two were non-nutrient transport media (RTF and VMG IV). The three others (TG, PY, PYG) were nutrient media. An increase in the transport time, and manipulations in an aerobic atmosphere compromised the survival of the bacteria. PY ensured good survival of the bacteria. RTF seems to be the best medium for the transport of P. intermedia, when quantitative analysis is required.

Adult

In vitro survival of Bacteroides intermedius in five transport media, alone or in the presence of Streptococcus sanguis.

The survival rate of Bacteroides intermedius was first tested in monoculture, and Streptococcus sanguis was then added in 5 different transport media; 2 nonnutritious media, the viability-preserving medium of the University of Göteborg No. IV (VMG IV), reduced transport fluid (RTF), and 3 nutritious media, thioglycolate medium (TG), peptone yeast extract medium (PY) and PY medium with 1% glucose (PYG). All manipulations were carried out in an anaerobic chamber. After a given transport time (6 or 24 h) aliquots were spread on plates containing solid PY medium by means of an automated spiral system device, thus permitting counts after incubation. The slight variations in the counts of B. intermedius in monoculture, not exceeding 0.5 log10 in the 5 media tested, indicated its good survival capability, i.e. at least 24 h. By contrast, when S. sanguis was added, it was only possible to use nutritious media such as PY or TG for 6 h. In the glocose-containing media (PYG, TG), the multiplication of rapidly glucose-fermenting microorganisms such as streptococci influenced the B. intermedius survival rate. Therefore, the transport time for oral microbiological samples needs to be reduced as much as possible. The use of excessively rich media (particularly media containing a high level of glucose) should be avoided.

Antibiosis

[Titularization].

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Calcium Hydroxide

[Root anatomy].

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Dental Pulp Cavity